LOCUS       sequence-384694-        3592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HA-tagged AP-4 epsilon in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Mattera R, Park SY, De Pace R, Guardia CM, Bonifacino JS
  TITLE     AP-4 mediates export of ATG9A from the trans-Golgi network to 
            promote autophagosome formation.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Dec 12;114(50):E10697-E10706. doi: 
            10.1073/pnas.1717327114. Epub 2017 Nov 27.
  PUBMED    29180427
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1717327114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-12-12- 12"; volume: "114"; issue: "50"; pages: 
            "E10697-E10706"
COMMENT     Sequence Label: pCI-neo-(HA)3-epsilon
FEATURES             Location/Qualifiers
     source          1..3592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             43..69
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             73..99
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             2410..2421
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 taatacgact cactataggc tagcctcgag gccgccacca tgtacccgta cgatgttcct
       61 gactatgcgg gctatcccta tgacgtcccg gactatgcca tgggctaccc ttacgacgtt
      121 ccagattacg ctgaattcat gagcgacata gtggagaaga cgctgacggc gctgccggga
      181 ctctttctgc agaaccagcc cggtggtggg cccgcggccg ccaaggcgtc cttctcctcg
      241 aggctgggca gccttgtccg cggcatcaca gccctcacct ccaagcacga agaagaaaaa
      301 ttaatccagc aggaactgag tagtctgaaa gcgactgttt ctgctcctac tacaacactg
      361 aaaatgatga aggaatgtat ggtgagactt atatattgtg aaatgcttgg atatgatgct
      421 tcctttggct atattcatgc aatcaagtta gcccaacaag gaaacctctt agaaaaaaga
      481 gtaggttatt tggctgtttc cttatttcta catgaaagtc atgaattatt gcttctcctt
      541 gtgaatacag ttgtaaagga tctgcagagc actaacctag tagaagtgtg tatggcactg
      601 actgttgtta gccagatttt cccctgcgaa atgattccag ctgttcttcc attaatagaa
      661 gataaacttc aacattctaa ggagattgta cgaagaaaag ctgttctggc attatacaaa
      721 ttccatctca ttgctcctaa tcaagtacaa catattcata ttaagtttcg gaaagcactt
      781 tgtgacagag atgttggggt catggctgcc tccttgcata tatatcttag aatgattaag
      841 gaaaattcat ctggatataa agacttgact gggagttttg taaccatttt gaagcaagta
      901 gttggaggaa agctcccagt agagttcaat taccacagtg tgccagcacc atggttacaa
      961 attcagctct tgagaatact gggacttcta ggaaaagatg atcaaaggac aagtgaatta
     1021 atgtatgatg ttcttgatga atccttacga agagctgagt taaatcacaa tgtcacatat
     1081 gctattttgt ttgaatgtgt gcatacagtc tattctattt atcctaaatc ggaattactt
     1141 gagaaggctg ccaagtgcat tggaaaattt gttctgtcac ctaaaataaa tctaaaatat
     1201 ttaggactga aggctcttac ctatgttatc caacaggatc ctactctggc tcttcaacac
     1261 cagatgacaa taattgaatg tttagatcat cctgatccca ttattaaaag agagactctg
     1321 gaacttcttt acagaattac taatgcacag aatataacag ttattgtcca gaaaatgctt
     1381 gaatatttac atcagagcaa agaagagtat gtcatcgtca atttggtcgg caaaatagca
     1441 gagctggctg agaaatatgc tcctgataat gcatggttta ttcagacaat gaatgctgtg
     1501 ttttcagtag gaggagatgt aatgcatcct gatattccca ataactttct gagactacta
     1561 gcggaaggtt ttgatgatga aacagaagat cagcaattaa gactctatgc agttcagtct
     1621 tatctcactt tactggatat ggaaaatgtg ttctatccac agagatttct tcaagttatg
     1681 agttgggtat taggggaata ttcctacctc ttagataagg aaacgccaga ggaagttata
     1741 gctaagctct acaagttact tatgaatgac tctgtgtctt cagaaacaaa agcctggtta
     1801 attgctgctg tgaccaaatt gacatctcag gcgcactctt ctaatacagt tgagagatta
     1861 atccatgaat ttaccatatc tttggatact tgtatgagac aacatgcatt tgaattaaaa
     1921 catttgcatg agaatgtgga acttatgaag agcttgcttc cagttgacag gagttgtgaa
     1981 gacttggtgg tagatgcttc tttatctttt ctggatggtt ttgtggctga aggactcagt
     2041 cagggtgcag cgccttacaa acctccccat caacgccagg aggaaaagct ttctcaggaa
     2101 aaagttctca attttgaacc atatggactc tccttttctt catctggctt cactggacga
     2161 cagtctcctg ctggcatttc tcttggttca gatgtatctg ggaatagtgc tgagacagga
     2221 ctgaaagaga caaatagctt gaagctggaa ggtataaaga aattgtgggg gaaagaaggc
     2281 tatcttccca agaaggaaag caaaactggt gatgaaagtg gagctctgcc tgttcctcaa
     2341 gagagtataa tggagaatgt agatcaagct ataactaaaa aggatcaatc tcaagttctt
     2401 acccaatcta aagaggagaa agaaaagcag ctgctggcat catcattatt tgttggtcta
     2461 ggatcagaaa gtacaatcaa cctgctggga aaagcagata ctgtctctca caagttcaga
     2521 aggaaatcaa aagtcaaaga agctaaaagt ggcgaaacaa ccagtactca taatatgacc
     2581 tgttcttcct ttagttcttt gtcaaatgtg gcatatgaag atgattatta ttcgaatact
     2641 ttgcacgata caggagacaa ggaattaaag aaattttctc tcacttcaga acttttggat
     2701 tctgagtcac tcacagaact gcccttggtt gagaaattct catattgtag tctgtctaca
     2761 ccttcattgt ttgctaataa caacatggaa atttttcacc ctcctcaatc tactgcagcc
     2821 tcagttgcca aggaaagctc tttagcttca tcctttttgg aagaaactac tgaatacata
     2881 cactcaaatg ctatggaagt ctgtaataat gaaactatat cagtgtcttc ttataaaatt
     2941 tggaaagatg attgtttatt gatggtctgg tcagtcacta ataagagtgg tttggaattg
     3001 aaaagtgctg acttagaaat ttttcctgca gaaaatttca aggtgactga gcaacctgga
     3061 tgctgtttgc ctgtaatgga agcagaaagc accaaaagct ttcaatatag tgtgcagata
     3121 gaaaaacctt ttacagaagg aaatcttact ggttttatta gttatcatat gatggatact
     3181 cattctgctc agctggaatt ttctgtaaac ttatcactat tagatttcat tagaccatta
     3241 aaaatctcaa gtgacgactt tgggaaactc tggttatcct tcgcaaatga tgtgaaacaa
     3301 aatgtaaaaa tgtcagaatc tcaagctgca cttccttctg cactaaagac tctgcaacag
     3361 aaactaagac tccatattat tgagattata ggcaatgaag ggctattggc ctgtcagctg
     3421 ctcccatcca tcccctgctt actgcattgc cgagttcatg cagatgtatt agccctgtgg
     3481 ttcagatcct cctgttctac tcttcctgac tatttactgt atcaatgtca aaaggtgatg
     3541 gagggatcct aggtcgactc gagcggccgc ttccctttag tgagggttaa tg
//
