LOCUS       sequence-384708-        4662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized Sc++ and blasticidin resistance: EFS
            promoter-Sc++-NLS-FLAG-P2A-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Kim N, Choi S, Kim S, Song M, Seo JH, Min S, Park J, Cho SR, Kim HH
  TITLE     Deep learning models to predict the editing efficiencies and 
            outcomes of diverse base editors.
  JOURNAL   Nat Biotechnol. 2024 Mar;42(3):484-497. doi: 
            10.1038/s41587-023-01792-x
  PUBMED    37188916
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2024-03"; volume: "42"; issue: "3"; pages: 
            "484-497"
COMMENT     Sequence Label: Sc++
FEATURES             Location/Qualifiers
     source          1..4662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4126..4173
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4174..4197
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4207..4263
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4264..4662
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 atggaaaaga aatatagcat tgggctggac attggaacaa actccgtggg ctgggctgtg
       61 attactgatg attacaaagt gccttcaaag aagtttaagg tgctgggcaa caccaataga
      121 aagtctatca agaagaacct gatgggcgcc ctgctgttcg acagcggaga gacagccgag
      181 gcaacccggc tgaagagaac agcccggaga aggtacaccc gccggaagaa ccggatcaga
      241 tacctccagg agattttcgc caatgagatg gccaagctgg acgatagctt ctttcagaga
      301 ctggaggagt ccttcctggt ggaggaggac aagaagaacg agaggcaccc aatctttggc
      361 aatctggccg atgaggtggc ctatcacaga aattacccta ccatctatca cctgaggaag
      421 aagctggccg atagcccaga gaaggccgac ctgcgcctga tctacctggc cctggcccac
      481 atcatcaagt tccggggcca ctttctgatc gagggcaagc tgaacgccga gaacagcgac
      541 gtggccaagc tgttctacca gctgatccag acatataacc agctgtttga ggagtctccc
      601 ctggatgaga tcgaggtgga cgccaagggc atcctgagcg ccagactgtc taagagcaag
      661 aggctggaga agctgatcgc cgtgttccca aacgagaaga agaatggcct gtttggcaat
      721 atcatcgccc tggccctggg cctgacccca aacttcaagt ccaattttga cctgacagag
      781 gatgccaagc tccagctgtc taaggatacc tacgacgatg acctggacga gctgctgggc
      841 cagatcggcg accagtatgc cgatctgttc tctgccgcca agaacctgag cgatgccatc
      901 ctgctgtccg acatcctgag atccaattct gaggtgacaa aggcccctct gagcgccagc
      961 atggtgaagc ggtacgatga gcaccaccag gacctggccc tgctgaagac cctggtgcgg
     1021 cagcagttcc cagagaagta tgccgagatt ttcaaggatg acacaaagaa cggctacgcc
     1081 ggctatgtgg gcgccgacaa gaagctgagg aagcgcagcg gcaagctggc caccgaggag
     1141 gagttctaca agtttatcaa gcctatcctg gagaagatgg acggagcaga ggagctgctg
     1201 gccaagctga acagggatga cctgctgagg aagcagcgca catttgataa tggcagcatc
     1261 cctcaccaga tccacctgaa ggagctgcac gccatcctga gaaggcagga ggagttctac
     1321 ccatttctga aggagaacag agagaagatc gagaagatcc tgaccttcag aatcccctac
     1381 tatgtgggac ctctggccag gggcaactct cgctttgcct ggctgaccag aaagagcgag
     1441 gaggccatca caccctggaa tttcgaggag gtggtggaca agggagccag cgcccagtcc
     1501 ttcatcgaga ggatgacaaa ctttgatgag cagctgccaa ataagaaggt gctgcccaag
     1561 cactccctgc tgtacgagta tttcacagtg tacaatgagc tgaccaaggt gaagtatgtg
     1621 acagagcgga tgagaaagcc tgagttcctg tctggcgagc agaagaaggc catcgtggac
     1681 ctgctgttta agacaaaccg caaggtgacc gtgaagcagc tgaaggagga ttacttcaag
     1741 aagatcgagt gctttgacag cgtggagatc atcggcgtgg aggatcggtt taatgcctcc
     1801 ctgggcacct atcacgacct gctgaagatc atcaaggata aggacttcct ggataacgag
     1861 gagaatgagg acatcctgga ggacatcgtg ctgaccctga ccctgtttga ggaccgcgag
     1921 atgatcgagg agcggctgaa gacctacgcc cacctgttcg atgacaaagt gatgaagcag
     1981 ctgaagcgcc ggcactatac aggatggggc cggctgagca gaaagatgat caacggcatc
     2041 agagataagc agtccggcaa gaccatcctg gattttctga agtctgacgg cttcagcaac
     2101 aggaacttca tgcagctgat ccacgatgac agcctgacat tcaaggagga gatcgagaag
     2161 gcccaggtgt ctggccaggg cgatagcctg cacgagcaga tcgccgacct ggcaggctcc
     2221 ccagccatca agaagggcat cctccagacc gtgaagatcg tggacgagct ggtgaaagtg
     2281 atgggccaca agcccgagaa catcgtgatc gagatggccc gcgagaatca gaccacaacc
     2341 aagggcctcc agcagtccag ggagcgcaag aagcggatcg aggagggcat caaggagctg
     2401 gagtctcaga tcctgaagga gaaccccgtg gagaataccc agctccagaa cgagaagctg
     2461 tacctgtact acctccagaa tggcagagac atgtatgtgg atcaggagct ggacatcaac
     2521 aggctgagcg attacgacgt ggatcacatc gtgccccagt ctttcatcaa ggatgacagc
     2581 atcgacaata aggtgctgac acggtccgtg gagaacaggg gcaagtccga taatgtgcct
     2641 tctgaggagg tggtgaagaa gatgaagaac tattggcgcc agctgctgaa tgccaagctg
     2701 atcacccagc gcaagtttga caacctgaca aaggcagaga ggggaggcct gtctgaggca
     2761 gataaggccg gcttcatcaa gagacagctg gtggagacaa ggcagatcac caagcacgtg
     2821 gccagaatcc tggacagccg gatgaacacc aagcgggaca agaatgataa gcctatccgg
     2881 gaggtgaaag tgatcaccct gaagtccaag ctggtgtctg atttccgcaa ggactttcag
     2941 ctgtacaagg tgcgggacat caacaattac caccacgccc acgatgccta tctgaacgcc
     3001 gtggtgggca ccgccctgat caagaagtat ccaaagctgg agagcgagtt cgtgtacggc
     3061 gattataagg tgtacgacgt gagaaagatg atcgccaagt ctgagcagga gatcggcaag
     3121 gccaccgcca agaggttctt ttacagcaac atcatgaatt tctttaagac agaggtgaag
     3181 ctggccaacg gagagatcag gaagcggccc ctgatcgaga caaacggcga gacaggcgag
     3241 gtggtgtgga ataaggagaa ggactttgcc accgtgagaa aggtgctggc catgccccaa
     3301 gtgaatatcg tgaagaagac agaggtgcag accggcggct tctctaagga gagcatcctg
     3361 tccaagaggg agtccgccaa gctgatcccc cgcaagaagg gctgggatac ccggaagtac
     3421 ggcggctttg gctcccctac agtggcctat tctatcctgg tggtggccaa ggtggagaag
     3481 ggcaaggcca agaagctgaa gtccgtgaag gtgctggtgg gcatcaccat catggagaag
     3541 ggctcttacg agaaggaccc catcggcttc ctggaggcca agggctataa ggacatcaag
     3601 aaggagctga tcttcaagct gcctaagtac agcctgtttg agctggagaa cggcagaagg
     3661 cgcatgctgg cctccgccaa ggagctccag aaggccaatg agctggtgct gccacagcac
     3721 ctggtgcgcc tgctgtacta tacacagaac atcagcgcca caaccggctc caacaatctg
     3781 ggctacatcg agcagcaccg ggaggagttc aaggagattt tcgagaagat catcgacttc
     3841 tccgagaagt atatcctgaa gaacaaggtg aactctaatc tgaagagctc cttcgatgag
     3901 cagtttgccg tgtctgacag catcctgctg tccaattctt tcgtgtccct gctgaagtac
     3961 accagctttg gcgcctccgg cggcttcaca tttctggacc tggatgtgaa gcagggcagg
     4021 ctgcgctatc agacagtgac cgaggtgctg gatgccaccc tgatctacca gagcattacc
     4081 ggactgtatg aaactcggac tgacctgagc cagctggggg gggacaagcg acctgccgcc
     4141 acaaagaagg ctggacaggc taagaagaag aaagattaca aagacgatga cgataaggga
     4201 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     4261 ccgatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     4321 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     4381 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     4441 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     4501 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     4561 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     4621 attcgtgaat tgctgccctc tggttatgtg tgggagggct aa
//
