LOCUS       sequence-38471-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pZW12 (CK2beta)
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     158..174
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     158..174
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             191..220
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctctctg gctactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
      121 ctggaattct gcagatatcc atcacactgg cggccgctct agaactagtg gatccaccat
      181 ggcgtctatg gaacaaaagc tgattagcga agaggacctg aacaacggga attctcgagc
      241 agccgctgac gtgaagatga gcagctcaga ggaggtgtcc tggatttcct ggttctgtgg
      301 gctccgtggc aatgaattct tctgtgaagt ggatgaagac tacatccagg acaaatttaa
      361 tcttactgga ctcaatgagc aggtccctca ctatcgacaa gctctagaca tgatcttgga
      421 cctggagcct gatgaagaac tggaagacaa ccccaaccag agtgacctga ttgagcaggc
      481 agccgagatg ctttatggat tgatccacgc ccgctacatc cttaccaacc gtggcatcgc
      541 ccagatgttg gaaaagtacc agcaaggaga ctttggttac tgtcctcgtg tgtactgtga
      601 gaaccagcca atgcttccca ttggcctttc agacatccca ggtgaagcca tggtgaagct
      661 ctactgcccc aagtgcatgg atgtgtacac acccaagtca tcaagacacc atcacacgga
      721 tggcgcctac ttcggcactg gtttccctca catgctcttc atggtgcatc ccgagtaccg
      781 gcccaagaga cctgccaacc agtttgtgcc caggctctac ggtttcaaga tccatccgat
      841 ggcctaccag ctgcagctcc aagccgcc
//
