LOCUS       sequence-38473-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pGV15 (CK2alpha K68M)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     114..130
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     114..130
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gcgagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctccaccg cggtggcggc cgctctagaa
      121 ctagtggatc ctctgaaaca aggtcttacc cccagctgct tctgaacaca gtgactgcca
      181 gatctccaaa catcaagtcc agctttgtcc gccaacctgt ctgacatgtc gggacccgtg
      241 ccaagcaggg ccagagttta cacagatgtt aatacacaca gacctcgaga atactgggat
      301 tacgagtcac atgtggtgga atggggaaat caagatgact accagctggt tcgaaaatta
      361 ggccgaggta aatacagtga agtatttgaa gccatcaaca tcacaaataa tgaaaaagtt
      421 gttgttatga ttctcaagcc agtaaaaaag aagaaaatta agcgtgaaat aaagattttg
      481 gagaatttga gaggaggtcc caacatcatc acactggcag acattgtaaa agaccctgtg
      541 tcacgaaccc ccgccttggt ttttgaacac gtaaacaaca cagacttcaa gcaattgtac
      601 cagacgttaa cagactatga tattcgattt tacatgtatg agattctgaa ggccctggat
      661 tattgtcaca gcatgggaat tatgcacaga gatgtcaagc cccataatgt catgattgat
      721 catgagcaca gaaagctacg actaatagac tggggtttgg ctgagtttta tcatcctggc
      781 caagaatata atgtccgagt tgcttcccga tacttcaaag gtcctgagct acttgtagac
      841 tatcagatgt acga
//
