LOCUS       sequence-384747-        4626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BBRF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BBRF1
FEATURES             Location/Qualifiers
     source          1..4626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2505..2604)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2621..2640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2622..2640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2645..2661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2645..2661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3092..3883
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3976..4564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4465..4484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg ttcaacatga acgtggacga gagcgcctct ggcgccctcg gctcctcggc
      721 cattcctgtt caccccacgc cggcctcggt ccgacttttt gagatcctgc agggaaagta
      781 cgcctacgtc cagggacaga ccatctacgc caacctccgc aaccccggag tcttctcgag
      841 gcaggtgttt acccatttgt ttaaacgagc catctctcat tgcacgtacg atgacgtgct
      901 acatgactgg aacaagttcg aggcctgcat ccagaagcga tggccgagcg atgactcgtg
      961 tgcgagccgg tttcgtgagt ccaccttcga gtcgtggtcc acgaccatga agctgaccgt
     1021 gcgtgacctg ctgaccacca acatctaccg agtgctacac agccgctccg tgctctccta
     1081 tgagcgttat gtggactgga tctgcgccac cggcatggtg cccgccgtta agaagcccat
     1141 aacccaagag ctccactcca agataaagag cctgagggac aggtgcgtct gtcgggaatt
     1201 ggggcacgag aggaccatca ggagtatcgg gacggaatta tatgaggcaa cgaaggaaat
     1261 aatagagtcg ctcaactcca cgttcatccc ccagtttacg gaggtgacca tcgagtacct
     1321 tccgaggagc gacgagtatg tggcctacta ctgtggccgc cgcatcaggc tgcatgtgct
     1381 cttccccccg gccatctttg ccggaacggt gaccttcgac agcccggtgc agcgcctcta
     1441 ccagaacatt ttcatgtgct accgcacgct ggagcatgcc aagatctgcc agctcctgaa
     1501 cacggcccct ctcaaggcca tcgtgggcca cggggggcga gacatgtaca aggacatcct
     1561 ggcccatctg gagcagaact cacagcgcaa ggaccccaag aaggagctgc tgaacctgct
     1621 ggtcaagctc tcggagaaca agaccatcag cggggtcacg gacgtggtgg aggagttcat
     1681 aacggatgcc tccaacaacc tggtggaccg caaccgtcta tttggccagc ccggggagac
     1741 agctgcacag ggcctaaaga aaaaggtctc caacacggtg gtcaagtgtc tgactgatca
     1801 gataaacgag caatttgacc agattaatgg cctagagaag gagagggagc tctatctaaa
     1861 gaagatccgc tccatggagt ctcagctgca ggcctccctg ggtcccggcg gcaacaaccc
     1921 agcggcgtca gcccccgccg cagttgcggc agaagccgcg tctgtagata tactgacggg
     1981 cagcaccgcc tccgcaatcg aaaagctgtt caactccccg tccgccagcc tgggtgccag
     2041 ggtgtctggt cacaatgaaa gcatcctaaa cagtttcgtt tctcaataca tccccccttc
     2101 gcgggaaatg actaaggatc tgactgaact ttgggaaagc gagctgttta acaccttcaa
     2161 gttaacaccc gtggttgata atcaggggca gcgtctctac gtcagatact cgtcagacac
     2221 gatctctata ttattgggcc ccttcaccta tctggtggca gagctttcac cggtggaact
     2281 cgtgacagat gtctacgcca ccctaggcat cgtggagatc atcgacgagc tctaccggag
     2341 cagtcgcctg gccatctaca tcgaggacct cggtcgaaaa tactgccccg cgagcgcgac
     2401 cgggggagat catggcatcc ggcaagcacc atcagcccgg ggggacacgg agcctgacca
     2461 tgcaaaaagt aagcctgcgc gtgacccccc gcctggtgct ggaagtccaa ctttcttgta
     2521 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2581 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2641 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2701 tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc
     2761 gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
     2821 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     2881 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     2941 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     3001 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     3061 gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc
     3121 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     3181 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     3241 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     3301 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     3361 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     3421 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     3481 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     3541 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     3601 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     3661 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     3721 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     3781 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     3841 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg
     3901 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3961 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4021 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4081 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4141 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4201 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4261 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4321 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4381 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4441 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4501 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4561 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4621 catgtt
//
