LOCUS       sequence-384751-        8535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Purification of SARM1 from E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8535)
  TITLE     Wu Lab 2022
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDB-His-MBP-SARM1
FEATURES             Location/Qualifiers
     source          1..8535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(84..103)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     294..315
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(544..1359)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(544..1359)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1267..1286
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1351..1370
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1481..2069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1970..1989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2223..2240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2255..2394
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2320..2339)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2480..2502
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2496..2687)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3496..4578)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4540..4559
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4579..4656)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4862..4881
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4965..4984
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4965..4983
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4984..5008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5023..5045
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5064..5081
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5088..6185
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      6042..6051
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     6159..6182
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             6213..6233
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(8434..8452)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8448..8495
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      join(8532..8535,1..452)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
       61 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      121 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      181 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      241 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      301 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      361 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
      421 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
      481 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
      541 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
      601 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
      661 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
      721 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
      781 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
      841 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
      901 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
      961 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     1021 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     1081 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     1141 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     1201 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     1261 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     1321 cggcctagag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     1381 tatgtaagca gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1441 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1501 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1561 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1621 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1681 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1741 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1801 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1861 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1921 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1981 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2041 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2101 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2161 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2221 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     2281 atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc
     2341 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     2401 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2461 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2521 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     2581 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     2641 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     2701 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     2761 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     2821 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     2881 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     2941 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     3001 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     3061 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     3121 ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc
     3181 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
     3241 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
     3301 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
     3361 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     3421 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
     3481 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3541 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
     3601 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
     3661 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
     3721 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc
     3781 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
     3841 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
     3901 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
     3961 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
     4021 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
     4081 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
     4141 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
     4201 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
     4261 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
     4321 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
     4381 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     4441 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     4501 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     4561 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     4621 cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat
     4681 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
     4741 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     4801 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     4861 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     4921 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
     4981 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
     5041 ggagatatac catgggcagc agccatcatc atcatcatca cggtaccaaa actgaagaag
     5101 gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta
     5161 agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag
     5221 agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg
     5281 accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt
     5341 tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg
     5401 cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc
     5461 cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga
     5521 gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg
     5581 ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata
     5641 acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga
     5701 atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga
     5761 ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa
     5821 cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag
     5881 gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc
     5941 tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga
     6001 agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg gaaaacgccc
     6061 agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta
     6121 ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc
     6181 agactggtac cgattacgat atcccaacga ccgaaaacct ttacttccag ggccatatgt
     6241 cgggcccacg gccgggcgcc gagcggctgg cggtgcctgg gccagatggg ggcggtggca
     6301 cgggcccatg gtgggctgcg ggtggccgcg ggccccgcga agtgtcgccg ggggcaggca
     6361 ccgaggtgca ggacgccctg gagcgcgcgc tgccggagct gcagcaggcc ttgtccgcgc
     6421 tgaagcaggc gggcggcgcg cgggccgtgg gcgccggcct ggccgaggtc ttccaactgg
     6481 tggaggaggc ctggctgctg ccggccgtgg gccgcgaggt agcccagggt ctgtgcgacg
     6541 ccatccgcct cgatggcggc ctcgacctgc tgttgcggct gctgcaggcg ccggagttgg
     6601 agacgcgtgt gcaggccgcg cgcctgctgg agcagatcct ggtggctgag aaccgagacc
     6661 gcgtggcgcg cattgggctg ggcgtgatcc tgaacctggc gaaggaacgc gaacccgtag
     6721 agctggcgcg gagcgtggca ggcatcttgg agcacatgtt caagcattcg gaggagacat
     6781 gccagaggct ggtggcggcc ggcggcctgg acgcggtgct gtattggtgc cgccgcacgg
     6841 accccgcgct gctgcgccac tgcgcgctgg cgctgggcaa ctgcgcgctg cacgggggcc
     6901 aggcggtgca gcgacgcatg gtagagaagc gcgcagccga gtggctcttc ccgctcgcct
     6961 tctccaagga ggacgagctg cttcggctgc acgcctgcct cgcagtagcg gtgttggcga
     7021 ctaacaagga ggtggagcgc gaggtggagc gctcgggcac gctggcgctc gtggagccgc
     7081 ttgtggcctc gctggaccct ggccgcttcg cccgctgtct ggtggacgcc agcgacacaa
     7141 gccagggccg cgggcccgac gacctgcagc gcctcgtgcc gttgctcgac tctaaccgct
     7201 tggaggcgca gtgcatcggg gctttctacc tctgcgccga ggctgccatc aagagcctgc
     7261 aaggcaagac caaggtgttc agcgacatcg gcgccatcca gagcctgaaa cgcctggttt
     7321 cctactctac caatggcact aagtcggcgc tggccaagcg cgcgctgcgc ctgctgggcg
     7381 aggaggtgcc acggcccatc ctgccctccg tgcccagctg gaaggaggcc gaggttcaga
     7441 cgtggctgca gcagatcggt ttctccaagt actgcgagag cttccgggag cagcaggtgg
     7501 atggcgacct gcttctgcgg ctcacggagg aggaactcca gaccgacctg ggcatgaaat
     7561 cgggcatcac ccgcaagagg ttctttaggg agctcacgga gctcaagacc ttcgccaact
     7621 attctacgtg cgaccgcagc aacctggcgg actggctggg cagcctggac ccgcgcttcc
     7681 gccagtacac ctacggcctg gtcagctgcg gcctggaccg ctccctgctg caccgcgtgt
     7741 ctgagcagca gctgctggaa gactgcggca tccacctggg cgtgcaccgc gcccgcatcc
     7801 tcacggcggc cagagaaatg ctacactccc cgctgccctg tactggtggc aaacccagtg
     7861 gggacactcc agatgtcttc atcagctacc gccggaactc aggttcccag ctggccagtc
     7921 tcctgaaggt gcacctgcag ctgcatggct tcagtgtctt cattgatgtg gagaagctgg
     7981 aagcaggcaa gttcgaggac aaactcatcc agagtgtcat gggtgcccgc aactttgtgt
     8041 tggtgctatc acctggagca ctggacaagt gcatgcaaga ccatgactgc aaggattggg
     8101 tgcataagga gattgtgact gctttaagct gcggcaagaa cattgtgccc atcattgatg
     8161 gcttcgagtg gcctgagccc caggtcctgc ctgaggacat gcaggctgtg cttactttca
     8221 acggtatcaa gtggtcccac gaataccagg aggccaccat tgagaagatc atccgcttcc
     8281 tgcagggccg ctcctcccgg gactcatctg caggctctga caccagtttg gagggtgctg
     8341 cacccatggg tccaacctag ctcgagcacc accaccacca ctgagatccg gctgctaaca
     8401 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     8461 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
     8521 tggcgaatgg gacgc
//
