LOCUS       sequence-384765-        4608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BXLF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BXLF1
FEATURES             Location/Qualifiers
     source          1..4608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2487..2586)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2603..2622)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2604..2622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2627..2643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2627..2643)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3074..3865
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3958..4546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4447..4466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gctggatttc caggaaagga ggccggcccg cccggcggct ggcgaaaatg
      721 tcaggaggat gagtcccctg aaaacgaaag acacgaaaac ttttacgctg agattgatga
      781 ctttgccccc tcagttctta ccccaaccgg tagtgactca ggggcaggtg aggaagatga
      841 cgacggcctc taccaggtgc ccacccactg gccccccttg atggctccga ccggactgtc
      901 tggggagagg gtgccgtgcc ggacccaggc tgccgtgaca tctaatactg gaaactctcc
      961 gggtagccgg cacacatcgt gcccctttac cctgcccagg ggagcccagc caccagcacc
     1021 cgcacaccaa aagcctactg cccctacccc gaagccccgt agccgggagt gtggccccag
     1081 caagacgcca gaccccttct cctggttccg caaaacttcg tgcacagaag gaggtgcgga
     1141 ctccacgagc cggagcttca tgtaccagaa aggctttgag gagggcctgg ccggccttgg
     1201 gctcgatgac aaatctgact gtgagtctga ggatgagtct aatttccgca ggccctcttc
     1261 tcattccgcg ttaaaacaaa agaatggtgg caagggtaag ccttctggtc tcttcgaaca
     1321 cctggccgcc catgggcgcg agtttagtaa gttatccaag catgctgccc agctgaagcg
     1381 gctaagtggg agtgtgatga atgttctgaa tctggatgat gcccaagaca cccgccaggc
     1441 caaggctcag aggaaggaga gcatgcgggt tccaattgtg acccacctaa caaatcatgt
     1501 accagtaatc aaacctgcct gttccctatt tttggaaggt gcccctggtg tgggaaagac
     1561 tactatgctg aatcatttga aggctgtctt tggggacctg accattgtag tccctgaacc
     1621 gatgcggtac tggactcatg tgtatgaaaa tgccattaag gccatgcaca agaatgtgac
     1681 tcgggcgagg catggaaggg aagacacgtc ggctgaggtt ttagcatgcc agatgaaatt
     1741 taccacccct ttcagagtct tggcctctag gaagcggagc ttgctggtta ccgagtctgg
     1801 tgccaggtca gtcgcgcccc tggattgttg gattttgcat gatcgccatt tgctgtcggc
     1861 ctccgtggtt ttccctctaa tgctgctccg gagccagctt ctctcctata gtgactttat
     1921 tcaagttttg gccacgttta ccgcagaccc gggggacacc atagtctgga tgaagttaaa
     1981 cgtggaggag aacatgcgcc gcctgaagaa gcgagggagg aaacatgagt caggactgga
     2041 tgctggctac ctgaagagtg tcaacgatgc atatcacgcc gtttactgtg cctggctcct
     2101 aacacagtat tttgcccccg aggacattgt gaaggtatgt gccggtctaa caacaatcac
     2161 gacagtatgt catcaaagcc acacccccat aattcgaagt ggtgtggccg agaagctgta
     2221 taagaacagc atctttagtg tacttaagga agtcatacag cctttccgag ccgacgcggt
     2281 gttgttggaa gtttgtcttg cgttcacccg aacgctagcc taccttcagt ttgtgcttgt
     2341 ggacctctca gagtttcagg acgatttacc tggatgctgg acggagattt acatgcaggc
     2401 attgaagaat ccggcaatac gatctcagtt tttcgattgg gctggtctga gcaaggtgat
     2461 ctctgatttt gagaggggaa atcgggaccc aactttcttg tacaaagttg gcattataag
     2521 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2581 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2641 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2701 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     2761 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     2821 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     2881 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2941 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     3001 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     3061 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     3121 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     3181 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     3241 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     3301 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     3361 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     3421 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     3481 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     3541 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     3601 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     3661 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     3721 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     3781 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     3841 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     3901 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4021 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4081 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
