LOCUS       sequence-38478-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pGP18 (CK2alpha' K69M)
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..194
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 agcgagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttccacca tgggccgcat cttttaccca tacgatgttc
      121 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg
      181 ttccagatta cgctgctcag tgcggccgcc ccggcccggc cgcgggcagc agggcccggg
      241 tctacgccga ggtgaacagt ctgaggagcc gcgagtactg ggactacgag gctcacgtcc
      301 cgagctgggg taatcaagat gattaccaac tggttcgaaa acttggtcgg ggaaaatata
      361 gtgaagtatt tgaggccatt aatatcacca acaatgagag agtggttgta atgatcctga
      421 agccagtgaa gaaaaagaag ataaaacgag aggttaagat tctggagaac cttcgtggtg
      481 gaacaaatat cattaagctg attgacactg taaaggaccc cgtgtcaaag acaccagctt
      541 tggtatttga atatatcaat aatacagatt ttaagcaact ctaccagatc ctgacagact
      601 ttgatatccg gttttatatg tatgaactac ttaaagctct ggattactgc cacagcaagg
      661 gaatcatgca cagggatgtg aaacctcaca atgtcatgat agatcaccaa cagaaaaagc
      721 tgcgactgat agattggggt ctggcagaat tctatcatcc tgctcaggag tacaatgttc
      781 gtgtagcctc aaggtacttc aagggaccag agctcctcgt ggactatcag atgtatgatt
      841 atagcttgga catgtggagt ttgggctgta tgttagca
//
