LOCUS       sequence-384812-        4602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BRLF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BRLF1(Rta)
FEATURES             Location/Qualifiers
     source          1..4602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1913..2482
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             1913..2482
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     protein_bind    complement(2481..2580)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2597..2616)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2598..2616)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2621..2637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2621..2637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3068..3859
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3952..4540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4441..4460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg aggcctaaaa aggatggctt ggaagacttt ctgaggctaa ctcctgaaat
      721 caaaaagcag ctgggctctc tggtctctga ctactgcaac gtcctcaaca aggaatttac
      781 agccgggagt gtggagatta ctctgagatc ctacaaaata tgcaaggcat ttataaatga
      841 ggccaaggcc cacgggcgag aatggggcgg gctaatggcc acgctcaaca tctgcaattt
      901 ttgggccatt ctccgaaaca acagggtaag aagacgggct gagaatgccg gcaacgacgc
      961 atgttccatc gcgtgcccta tagtgatgcg ctacgtgtta gaccacctga tagtggtcac
     1021 tgacagattc ttcatccagg cccccagcaa ccgggtgatg attcctgcca ccataggcac
     1081 cgctatgtac aagctcctaa aacacagtcg ggtgcgggcc tacacctaca gcaaggtgct
     1141 gggcgtggac cgcgcggcca tcatggcctc cggcaagcag gtagtggaac acctgaacag
     1201 gatggagaag gaaggcctcc taagctccaa gttcaaggcc ttttgcaagt gggtgttcac
     1261 ctatcccgtc ctcgaggaga tgttccagac tatggtctcg tccaagacag gccatctgac
     1321 ggacgatgtt aaggatgtca gggctctgat taagacactg ccccgggcct cctactccag
     1381 ccacgccgga cagaggagct acgtgagcgg cgtgcttccc gcgtgcctgc tgtcaaccaa
     1441 gtccaaggca gtggaaactc ctatcctcgt gtccggagcc gacaggatgg acgaggagct
     1501 catggggaat gatgggggtg cctctcacac cgaggcccgc tactcggagt ccggacagtt
     1561 tcatgctttt acagatgaac tcgaaagtct cccgagcccg accatgcccc tgaagcccgg
     1621 tgcccaaagc gccgactgcg gtgacagcag ttccagcagc agtgactcgg gcaacagtga
     1681 caccgagcag agcgagcggg aagaggccag ggccgaggcc ccgcgcctgc gggccccaaa
     1741 gtcgcgccgg acatccaggc ccaaccgtgg tcaaactcca tgtccttcca acgcggcgga
     1801 acctgaacag ccttggatag cagcggtcca ccaagagagc gatgagagac ccatattccc
     1861 ccacccctca aagcccacct ttcttcctcc cgttaaaagg aagaagggcc tcagggatag
     1921 ccgggaaggt atgttcctgc caaagccgga agcgggcagt gccatatctg acgtgttcga
     1981 ggggcgagag gtgtgtcagc caaagaggat caggcccttc catccacccg gatccccgtg
     2041 ggccaaccgg cccctgcctg cctctttggc tcccaccccc acaggacctg tccatgaacc
     2101 ggtcggatcc ctaacgccag ccccggtgcc ccagccactt gacccggccc ccgcagtaac
     2161 ccccgaggca agtcatctgt tggaggaccc tgatgaagaa accagtcagg ccgtgaaggc
     2221 cctaagggag atggctgaca ctgttattcc ccagaaggag gaagcagcca tatgtggaca
     2281 gatggacctg agccacccgc cccctcgtgg ccatttggac gaactgacca caacactaga
     2341 gtccatgaca gaggatttga atctggactc ccccctgacc cccgaactta atgaaatctt
     2401 ggatacattt ctaaatgatg aatgtctgct gcatgccatg catatttcaa ctgggctgtc
     2461 tatttttgac accagcttat ttccaacttt cttgtacaaa gttggcatta taagaaagca
     2521 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2581 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2641 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2701 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2761 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2821 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2881 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2941 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     3001 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     3061 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     3121 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     3181 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3241 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3301 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3361 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3421 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3481 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3541 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3601 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3661 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3721 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3781 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3841 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3901 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3961 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4021 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4081 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4141 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4201 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4261 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4321 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4381 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4441 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4501 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4561 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
