LOCUS       sequence-384838-        9742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 
            of the C. elegans genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   Kurashina M, Mizumoto K
  TITLE     Targeting endogenous proteins for spatial and temporal knockdown 
            using auxin-inducible degron in Caenorhabditis elegans.
  JOURNAL   STAR Protoc. 2023 Jan 13;4(1):102028. doi: 
            10.1016/j.xpro.2022.102028.
  PUBMED    36640369
REFERENCE   2  (bases 1 to 9742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2023-01-13"; volume: "4(1):102028. doi"; pages: " 
            10.1016/j.xpro.2022.102028"
COMMENT     Sequence Label: ChrV_oxTi365_loxP_myo2_neoR
FEATURES             Location/Qualifiers
     source          1..9742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     285..302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    419..440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(455..460,461..478,479..485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    493..509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     498..520
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     517..533
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     517..533
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1520..1553
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2283..3077)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2394..2413)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3004..3023
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3899..3915
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4977..4997)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     5745..5773
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(5806..5830)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    6851..6884
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7659..7675)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7668..7690)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7884..7903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8003..8025
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8063..8081)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8149..8253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8254..8322,8323..9114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8472..8491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9285..9742,1..131)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      361 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      421 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      481 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      541 acgccaagct tgcggccgcg gaatgtgcgt ggtgtttgta aaatcgtaat aatcacataa
      601 atacaatcaa ctcaaaatga tgaaaccagc aaaaatagaa acaagaaaat gcgattcaag
      661 tgaatagggc aacattcctc ttcatctcat tcttcaacta aatttaattt ggcttccagt
      721 tttttctcta ttcttttccg tcttctttcg ttccttttcc aattgcactt tttgtaccat
      781 agggaccctc cgtcttcgtt tttttctgca aacaaaagcg ccaccctaac tatcattccg
      841 ctttacaaag gaaatgagaa gaacgaactg ggagagccca gggtggcctt cttcttcgtt
      901 tggtttttta agagtctcgg gtcccgaggg tccattcaaa attgagaaca tgctgttttg
      961 tagaaaacga aaggcgacat tccgcaagat ttgggtggtc tccgacgaga gacgtttagc
     1021 ccgattgcct gatttatgac catgtgcact tttacagact tgtttagtgt caatatgaaa
     1081 aacgaatgtg caaaactggt tgatgaaagg caagcgtgaa ctagtacgtg tgaatctgac
     1141 atctaaagtt gaattataaa aaactcgtag ggaaaatacg gtaatccatt tatcagactc
     1201 aattcaaata aatattctga aacacagtat cagagttcat aatctgatgt tgcaatgtta
     1261 tcagtgaatt caattagtta gaatgtttcg ccgataaccc tatttagttt tctttcccat
     1321 ttcagcggag aataattatt ttttcattca gtgtttcaat ttatttttat ttttcagaaa
     1381 atgggtatta tttccattat tttccttctt attgggtctt cgacggctgc agtcctcgaa
     1441 catcaattaa tatggaaaga atctagaatg attgaaatga tcagaaatgg agaatatcct
     1501 gcctagtggt cctgcaacta taacttcgta taatgtatgc tatacgaagt tatgtcgacg
     1561 catgcaggcc tgtcgacctc gagaaacagt tatgtttggt atattgggaa tgtattctgt
     1621 catttaaggc aaaaacccca tagacactac tccactttca aattatcaac ttctactcac
     1681 cttccactga gcctcaaacc caaaccttct tccgatcttt cttgcatcgt gctcatcaat
     1741 acttgtgatt ttagcaagaa aaacgccgtc ctcgaaatct tgacaaacaa taggggaaac
     1801 cccaaaaagc aaaagcagga aaattaattt cataaacttt cacttgattg aaaaattcca
     1861 aaaatactcc aaaacttttt cacgatgagc atgatttgac gtcatgagag gcccagacgt
     1921 gcgaagaaat aaaaattgcg gtcataaact gaaacgtaac atatgataag gtattttgtg
     1981 tgcggttttt tctatgatgt ttcaaaaaaa tttatcagaa gtaaaaaaca taagaagtgt
     2041 acacagaaca ttgtgtagat tttgggatag aattattatt aatgggggga gggagcacaa
     2101 tttttttcga ctttttatta cgaattaatt ctatttttga atctacacaa tttcattgtt
     2161 agaggtggct taaaagaagc taaaaaacaa agaaattaag agaagttttt tgataataac
     2221 aaaaataggg ggtgggagca cagggagaaa gagcatgtag ggatgttgaa gagtaattgg
     2281 actcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     2341 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     2401 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     2461 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     2521 acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggt
     2581 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     2641 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     2701 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     2761 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     2821 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     2881 cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     2941 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     3001 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     3061 ccatcttgtt caatcatttt tattccactt gttgagcagg gctggctgaa aatatgtgaa
     3121 ttgagaaaat aaatagtata tttgaaggaa gggaagacga gataaacgga aaaacacgat
     3181 tgcggaacat tacgaaaaat ttcgaaattt attatttttt ttaataacat ttgtttaccg
     3241 gccggacaag acaaaccaac ttcgaaacga ggaataaatc aaatttcata tttaaaatga
     3301 atcaaaaccc atgattaaac gaaatgacaa aaatcgataa cgtgtataga acgaactcaa
     3361 gctgaaccga gaaccgagaa attcagggtt tttagtattt tattatttat tttagtttta
     3421 tttaagctga taatttcgaa acgttatgat ttgaataaaa atcgtggaat attgatattt
     3481 tcccaaaaat tccacttggt agaatcacaa atctaggcaa attggccgtt ttctatcaca
     3541 aattacgagc gaaaactaca aataatgcaa tttttatata cttttctaac taaaaacctg
     3601 aaaaaaatat cgaaaaaatc gggaaaacag ttgaaaaacg ggaaaaaaag ggttaaaatt
     3661 ttaggcccag caatttcatc gccttccgca aaccgtgcgg tggggcggag aatgtaatgg
     3721 tgcggcgcgg ttttcggagt gtcgtagcgc gctgtgttta tcgtaaatcg gcgcgagctc
     3781 ttttttgaag caattttttg gtttttattg aaagttagtc tcaaaactcg tattatttta
     3841 aaattttttt attaaactaa aatttagaaa tgttaaggtg ccatggccca tccggcctgt
     3901 aaaacgacgg ccagtccgta atacgactca cttaaggcct tgactagagg gtaccagagc
     3961 tcacctaggt atctagaacc ggtgacgtca ccatgggaag cttcgtggat ccagatatcc
     4021 tgcaggaatt cctcgagacg tacggtgcgc gcgatgcatt cgaagatcta aacagttatg
     4081 tttggtatat tgggaatgta ttctgtcatt taaggcaaaa accccataga cactactcca
     4141 ctttcaaatt atcaacttct actcaccttc cactgagcct caaacccaaa ccttcttccg
     4201 atctttcttg catcgtgctc atcaatactt gtgattttag caagaaaaac gccgtcctcg
     4261 aaatcttgac aaacaatagg ggaaacccca aaaagcaaaa gcrggaaaat taatttcata
     4321 aactttcact tgattgaaaa attccaaaaa tactccaaaa ctttttcacg atgagcatga
     4381 tttgacgtca tgagaggccc agacgtgcga agaaataaaa attgcggtca taaactgaaa
     4441 cgtaacatat gataaggtat tttgtgtgcg gttttttcta tgatgtttca aaaaaattta
     4501 tcagaagtaa aaaacataag aagtgtacac agaacattgt gtagattttg ggatagaatt
     4561 attattaatg gggggaggga gcacaatttt tttcgacttt ttattacgaa ttaattctat
     4621 ttttgaatct acacaatttc attgttagag gtgacttaaa agaagctaaa aaacaaagaa
     4681 attaagagaa gttttttgat aataacaaaa atagggggtg ggagcacagg gagaaagagc
     4741 atgtagggat gttgaagagt aattggactt agaagtcaga ggcacgggcg cgagatgctg
     4801 aaaatttaaa tattactcgt tagttagtag aactcagttt aaacttactc tgatgacagc
     4861 ggcccctatt atttttgaca ccagacaagt tggtaatggt agcgaccggc gctcagttgg
     4921 aattctacga atgctatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta
     4981 caaactcaag aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt
     5041 gtgtggacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt
     5101 gttgataatg gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt
     5161 tctgaaaatt taaatcagat tagttagtta gtaaaatcat gtttaaactt acaactttga
     5221 ttccattctt ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca
     5281 atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat
     5341 taacaagggt atcaccttca aacttgactt cagcacctga aaatttaaat atgtatggtt
     5401 agttagtacc gaactgttta aacttacgtg tcttgtagtt cccgtcatct ttgaaaaata
     5461 tagttctttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgccgtttca
     5521 tatgatctgg gtatctcgag aagcattgaa caccataaca gaaagtagtg acaagtgttg
     5581 gctgaaaatt taaataatca gggttagtta gtatatatat gtttaaactt acccatggaa
     5641 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
     5701 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
     5761 gaattgggac aactccagtg aaaagttctt ctcctttact cattaagcct gcttttttgt
     5821 acaaacttgt catttctgtg tctgacgatc gagggttaaa atgaaaagtg gtggagagaa
     5881 gtcaaccgct cggtgggatg cgcgagcctg gagataactt tgattttcgg cgagcccttc
     5941 tttttccctg ggtgggtgga ttgggaggcg gggatatcca attgtaattc taggtagctg
     6001 aaaagtgtct gcacactgtt ctctgaccat cgacgcagga aaagtctctt tctcaagtgc
     6061 aaatagcaaa aatcttcttt cttccccccc atcttccgag caaggcgccc ccacggatag
     6121 ttgctcgctg gcggtaggga gcgaagagag cagagaacct caacgggata gtggggatac
     6181 gagaaaagaa acggcatttt aggttaacta aagatagtga agaagccggc tcaatgcaga
     6241 gaggcagaca tccgacggca aacaacgaga gaattttatc cagaacaatg agtacagtgt
     6301 gtgcgcgcaa agtgctcttc ctgccatttt gctactctta tccacacaac cactttagcg
     6361 attaccaacc tatcttgaga cttatcttta ttttctcttc ttttgccgac gaaacgggag
     6421 agaggggcaa aaaatgaaga atcgggggca ctgattaaca cacttacttt tttttaggct
     6481 gaagcacaca ttgctgttcg gggtgtaaag ttcagcagaa ttcaaagcaa aagaccaccc
     6541 gcatcggtag tcaatattga tctggaagat tattaaaaat tgtcagagtg agccatatgc
     6601 atatgaattt aattcataca taacatttat aagacaggaa taaataccaa agtgaagtta
     6661 ttatttggtc gattatcact ttagcatagt gaaagtatcc ctcaatgtct ctacttgtaa
     6721 actcagttga tttacaaacg ttgttttatt gattataaaa ttagttttat ggacatttaa
     6781 agcaaaggta ctactcagat ataaaatgac tagtgagtcg tattacgtac gaggtaccga
     6841 gctctacgta ataacttcgt ataatgtatg ctatacgaag ttataggccg cctgatgccc
     6901 gggcatttgg agtaccgcga ccgtatggtt gctagtagat catctaacct ggctactgtg
     6961 gttcaaagca caacagatta tgtgtactat gagtacatgg gaaatattac tgttggaact
     7021 cctgatcaaa actttattgt tgtattagat accggatcag ccaatctctt aattccagga
     7081 actaattgca cgttagtatt cctgttttga aattgaataa cgtttgtgat cacaattttt
     7141 cgaaagtttt ttacagaacg tactgtgaga aaaagagatt atttaatgaa aaagcttcat
     7201 ccacatatat tgcaaccaac cgcccatggc agatcaaata tgctagcggt gatgcctacg
     7261 ggacattggg aattgacact gttaaggtta tttgatggaa attctgaaat tttacatatt
     7321 taaatgcata aataaattgt aaaaaagtat taacaaaaat ttaattagga gtaaattcgt
     7381 gtctggtact gtgaataact tttcttggag tcatctagga taaaatggaa ttttcagatc
     7441 ggtggatcag gagaagcaca actcgctatt ccaagatcat accttggtgt tgcagacact
     7501 gtcggatcag atttcaaatg ggttcgtttt tttcaagttc cttcactttc actattttgc
     7561 atttgcagag cccaaaagag ggaatctttg gtcttgcatt tactgcatta gctgttgaca
     7621 acattacccc tccaatcatc aacgctccgc gggaattcac tggccgtcgt tttacaacgt
     7681 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     7741 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7801 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     7861 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     7921 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     7981 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     8041 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8101 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8161 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8221 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8281 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8341 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8401 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8461 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8521 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8581 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8641 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8701 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8761 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8821 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8881 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8941 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9001 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9061 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9421 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9721 tatccggtaa gcggcagggt cg
//
