LOCUS       sequence-384839-        2111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  92-1 Op (Part 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS164
FEATURES             Location/Qualifiers
     source          1..2111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(102..690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(182..201)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             872..1531
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(938..957)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1602..1636
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ctacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
       61 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      121 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      181 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      241 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      301 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      361 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      421 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      481 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      541 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      601 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      661 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      721 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgattga tcgggcacgt
      781 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      841 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      901 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      961 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     1021 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     1081 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     1141 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     1201 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     1261 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtttcag ccaatccctg
     1321 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     1381 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     1441 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     1501 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
     1561 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
     1621 gccgggcgtt ttttattggt ctggtctcac atattagtgt gtgtatttgt gtttgtgtgt
     1681 ctatagaagt atagtaattt atgctgcaaa ggtcctaatg tataaggaaa gaatatttag
     1741 agaaaagaag aaaacaagag ttttatatac atacagagca catgcatgcc atatgatcat
     1801 gtgtcgtcgc acacatatat atatgcctgt atgtgtcagc actaaagttg cctggccatc
     1861 cacgctatat atacacgcct ggcggatctg cttaaggcca ttcatgccaa ggctacatct
     1921 attaatattc gaaggctaca tctattaata ttcgaaggct acatctatta atattcgaag
     1981 gctacatcta ttaatattcg aaggctacat ctattaatat tcgaaggcta catctattaa
     2041 tattcgaagg ctacatctat taatattcga aggctacact cgttggcctc aacctaggcg
     2101 tttgagaccg a
//
