LOCUS       sequence-384840-        4602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BVRF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BVRF2
FEATURES             Location/Qualifiers
     source          1..4602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2481..2580)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2597..2616)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2598..2616)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2621..2637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2621..2637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3068..3859
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3952..4540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4441..4460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gtgcaggcac cgtctgtata cgtctgcggc ttcgtggagc gcccggacgc
      721 cccacccaag gacgcctgcc ttcacctgga tcccctcacc gtcaagagcc agctccctct
      781 gaagaagccc ttgccactca cggtggaaca cctgccggat gctccggtcg gctcagtctt
      841 tggcctttac cagagccgag cgggtctctt tagcgcagcc tcgattacct ctggggactt
      901 cctgtccctg ctggactcaa tttaccacga ttgcgatatt gcacagagtc agcgcctgcc
      961 cctccctcga gaacccaagg tggaggctct gcacgcctgg ctcccctcac tgtcactggc
     1021 ctccctccac ccagacatac cccaaaccac cgcagatgga ggcaagctgt ccttctttga
     1081 ccacgtgtct atctgtgccc tgggtcgtcg gcgcggcacc acggcagtct acggtacaga
     1141 ccttgcgtgg gtcctgaagc actttagtga cctggaaccg tctatcgccg cccagattga
     1201 gaatgacgcc aatgccgcaa agcgtgaatc cggatgcccg gaagaccacc ctctgcccct
     1261 cacgaagctc atagctaagg caatcgatgc tggatttctg agaaaccgcg tggagactct
     1321 gaggcaggac aggggtgtgg ccaatatccc agccgagtcg tatttaaagg ccagcgacgc
     1381 cccggaccta caaaagccgg acaaggcact tcagagccca ccaccggcct ccacagaccc
     1441 agccaccatg ctatcaggta acgcaggaga aggagcaaca gcctgcggag gttcggccgc
     1501 cgcgggccag gacctcatca gcgtcccccg caacaccttt atgacactgc ttcagaccaa
     1561 cctggacaac aaaccgccga ggcagacccc gctaccctac gcggccccgc tgcccccctt
     1621 ttcccaccag gcaatagcca ccgcgccttc ctacggtcct ggggccggag cggtcgcccc
     1681 ggccggcggc tactttacct ccccaggagg ttactacgcc gggcccgcgg gcggggaccc
     1741 gggtgccttc ttggcgatgg acgctcacac ctaccacccc cacccacacc cccctccggc
     1801 ctactttggc ttgccgggcc tctttggccc ccctccaccc gtgcctcctt actacggatc
     1861 ccacttgcgg gcagactacg tccccgctcc ctcgcgatcc aacaagcgga aaagagaccc
     1921 cgaggaggat gaagaaggcg gggggctatt cccgggggag gacgccaccc tctaccgcaa
     1981 ggacatagcg ggcctctcca agagtgtgaa tgagttacag cacacgctac aggccctgcg
     2041 ccgggagacg ctgtcctacg gccacaccgg agtcggatac tgcccccagc agggcccctg
     2101 ctacacccac tcggggcctt acggatttca gcctcatcaa agctacgaag tgcccagata
     2161 cgtccctcat ccgcccccac caccaacttc tcaccaggca gctcaggcgc agcctccacc
     2221 cccgggcaca caggcccccg aagcccactg tgtggccgag tccacgatcc ctgaggcggg
     2281 agcagccggg aactctggac cccgggagga caccaaccct cagcagccca ccaccgaggg
     2341 ccaccaccgc ggaaagaaac tggtgcaggc ctctgcgtcc ggagtggctc agtctaagga
     2401 gcccaccacc cccaaggcca agtctgtgtc agcccacctc aagtccatct tttgcgagga
     2461 attgctgaat aaacgcgtgg ctccaacttt cttgtacaaa gttggcatta taagaaagca
     2521 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2581 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2641 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2701 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2761 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2821 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2881 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2941 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     3001 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     3061 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     3121 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     3181 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3241 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3301 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3361 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3421 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3481 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3541 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3601 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3661 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3721 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3781 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3841 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3901 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3961 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4021 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4081 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4141 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4201 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4261 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4321 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4381 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4441 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4501 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4561 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
