LOCUS       sequence-384862-        4398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BRRF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BRRF2
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2277..2376)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2393..2412)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2394..2412)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2417..2433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2417..2433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2864..3655
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3748..4336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4237..4256
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg agtgggcagc agagaggctc ggttattttg gttcctgaac atctggctgg
      721 ggcattaact aagcttatga gcgattttat cacaggacaa gatgtcactc tttctggagg
      781 aaatattgca gtcaaaattc gcgatgctat aaaccagacc cccgggggtg gtgatgtagc
      841 tatactttct tccctgtttg ctttatggaa tgccctccca acatctggta gacaatcctc
      901 cagggacgat ttaatcccag ccgccgtgca ggccttaacc acggcccaca acttatgtct
      961 gggtgttatt ccaggtgaga cctcacacaa ggacacaccc gagtcattgc tccgggctat
     1021 cgtgacgggt ctccaaaaat tgtgggtgga ttcgtgcgga tgtccagagt gcctacaatg
     1081 tcttaaggga ttgaaggcaa ttaagcccgg cctttatgaa atccctagga taataccaca
     1141 cactaagcag tgtagtcctg tcaatctcct gaacatgttg gtccacaagc ttgtggcttt
     1201 acgtggtcat gtgcagcttg catacgacgc ccgtgtcctg acgcctgact ttcacgaaat
     1261 ccctgacctc gatgactccg atgctgtttt cgcacgcacc ttattggcag ccttatttca
     1321 cctcaatatg ttctttattc tcaaagatta cataacacaa gactccatga gcttgaagca
     1381 ggccctcagt ggtcattgga tgtctgccac gggcaacccc ctgcctgcag caccggaaac
     1441 cctgcgagac tacttggaag ctttccgaaa ttcggataat cacttttatc tcccgacgac
     1501 agggccttta aacaccttcc aatttcccga agagcttctg gggcgcgttg ttgttattga
     1561 ttcctctttg tgtgccgcca gtcacgttca ggacgttatc acccatggtg ttggggcggg
     1621 tgttcctcgt cctcggtttt cggccctgcc tccggcccca tcccgcgagc cccagcagac
     1681 atgctctcag ttaacgagca gagggaatga aagctcacgg cgaaacttgg gccagcccgg
     1741 ggggacctcc cctgctgttc ccccagtttg ccccatcgtt tccctgacgg cctcaggggc
     1801 caagcaaaac cgcgggggca tgggatcctt gcacttagcc aagcctgagg aaacctcccc
     1861 cgccgtctcc ccagtatgcc ccatcgcttc cccagcggcc tccaggtcca agcagcactg
     1921 cggggtcact ggatcctcac aggccgcacc cagcttttct tccgttgccc cagtagcatc
     1981 tctgtctggt gaccttgaag aggaagagga ggggtcccga gaatccccat ccctaccgtc
     2041 cagcaaaaag ggggacgagg aatttgaggc ctggcttgag gctcaggacg caaatcttga
     2101 ggatgttcag cgggagtttt ccgggctgcg agtaattggt gatgaggacg aggatggttc
     2161 ggaggatggg gaattttcag acctggatct gtctgacagc gaccatgaag gggatgaggg
     2221 tgggggggct gttggagggg gcaggagtct gcactccctg tattcactga gcgtcgtccc
     2281 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2341 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2401 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2461 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     2521 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2581 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     2641 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     2701 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     2761 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2821 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     2881 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2941 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     3001 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     3061 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     3121 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     3181 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     3241 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3301 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3361 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3421 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3481 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3541 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3601 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     3661 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3721 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3781 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3841 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3901 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3961 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4021 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4081 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4141 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4201 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4261 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4321 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4381 gccttttgct cacatgtt
//
