LOCUS       sequence-384863-        4362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BFLF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BFLF1
FEATURES             Location/Qualifiers
     source          1..4362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2241..2340)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2357..2376)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2358..2376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2381..2397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2381..2397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2828..3619
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3712..4300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4201..4220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gctcacaaag tcacgagcgc aaacgagcca aaccccctga ccggaaaaag
      721 actgtccagc tgccccctca cgaggagtgg cgtaacagag gtggcacaga tcgcgggcag
      781 aactccaaag atggaggact ttgtgccctg gaccgtggac aatctgaaaa gccagttcga
      841 ggcagtcggc cttttgatgg cccactccta tcttccagcc aacgcggaag aaggcatagc
      901 ctacccacca ctcgtccaca cctacgagtc cctctctcca gccagcacct gccgagtttg
      961 tgatctgctc gacaccctgg tcaatcactc agatgccccc gtggccttct ttgaggacta
     1021 tgccctgctc tgctactact gtctaaatgc cccacgagcc tggatctcta gcctcatcac
     1081 aggcatggac tttttacaca tcctgataaa atacttcccc atggccgggg ggttggattc
     1141 cctcttcatg ccctccagga ttcttgccat tgacatccag cttcactttt acatttgccg
     1201 gtgcttcctc cctgtgtcct cttctgacat gattagaaat gccaacctcg gctactacaa
     1261 attggagttt ctgaaatcca tcctcaccgg ccagtccccg gccaacttct gcttcaagtc
     1321 catgtggccc agaaccaccc ccacttttct taccctccct ggacccagaa cctgcaaaga
     1381 ttcccaggat gtcccagggg acgtcggcag gggcctgtac accgccctgt gctgccacct
     1441 gcctaccaga aaccgagttc agcatccatt tctcagggcc gaaaagggag gactctctcc
     1501 tgaaattaca accaaggccg actactgtgg actactatta ggtacgtggc aggggacgga
     1561 cctgctcggg gggccaggcc accatgctat cggtttaaac gcggaataca gcggcgatga
     1621 gctggcggag ttggccctgg ccatcaccag accggaggct ggcgaccatt cgcagggccc
     1681 ctgcctcctg gcccccatgt ttggactaag gcacaagaat gcctcacgga ccatctgccc
     1741 actctgcgag agcctcgggg cccacccgga cgccaaggac acccttgacc gcttcaagag
     1801 cctgattctt gactcgttcg gcaacaacat caaaatccta gacaggatcg tgttcctcat
     1861 caagacccaa aacacgctgc tggatgtgcc atgccccagg ctaagggcct ggctgcagat
     1921 gtgcaccccg caagacttcc acaagcacct gttctgcgac cccctgtgtg ccatcaacca
     1981 cagcatcacg aatccgagcg tgctgtttgg tcagatttac ccacccagct tccaggcctt
     2041 taaggcagcg cttgcagccg gtcagaatct ggaacagggg gtctgtgact cgttaattac
     2101 gcttgtgtac atatttaaat ccacacaagt ggccagagtg ggcaaaacaa tcctcgtgga
     2161 tgtcactaag gaactggacg tggtcctgcg catccacggc cttgacctgg tacagtccta
     2221 tcaaacttcc caggtctacg tgccaacttt cttgtacaaa gttggcatta taagaaagca
     2281 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2341 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2401 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2461 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2521 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2581 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2641 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2701 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2761 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2821 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2881 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2941 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3001 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3061 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3121 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3181 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3241 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3301 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3361 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3421 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3481 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3541 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3601 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3661 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3721 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3781 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3841 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3901 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3961 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4021 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4081 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4141 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4201 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4261 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4321 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
