LOCUS       sequence-384864-        4308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BGLF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BGLF1
FEATURES             Location/Qualifiers
     source          1..4308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(960..971)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1662..1673
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(2187..2286)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2303..2322)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2304..2322)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2327..2343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2327..2343)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2774..3565
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3658..4246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4147..4166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gatgtccaca ttgacaacca agtgttgagt ggcctcggga cccccctcct
      721 cgtgcaccta tttgttcccg acacggttat ggcagagctt tgccccaatc gcgtgccaaa
      781 ctgcgagggg gcctggtgcc agactctctt cagtgaccgg acgggtctca cgagggtctg
      841 ccgcgtgttt gctgctcggg gcatgctgcc cggacggcct agccatcggg gcacgtttac
      901 cagtgtgcca gtgtactgcg atgagggcct tccagagctc tacaacccct tccacgtggc
      961 cgcccttcga ttttacgatg aaggagggct ggttggggag ctacagattt attacctgtc
     1021 tctctttgag ggggccaaaa gggctctgac cgacgggcat cttatcagag aggcctctgg
     1081 ggtccaggag tctgctgcgg ctatgcagcc catacctata gatcctgggc cccccggagg
     1141 ggcgggtata gagcatatgc cggtggccgc ggcccaggtc gagcacccta aaacgtatga
     1201 cctcaagcaa attctattgg agataacgca agaggagaat agaggggagc agaggttggg
     1261 ccacgctggg agtccggccc tttgcctggg gctcagactt agggccgggg ccgagaccaa
     1321 ggcggctgct gagacctctg tgtccaagca ccacccagcc ctcgagaacc ccagcaacat
     1381 ccggggcagc gccggtgggg aaggcggcgg aggccgagca gggactggcg gcactgtggg
     1441 ggtcgggtcg ggggcactct cgagggtgcc tgtttctttc tctaagaccc gtcgggccat
     1501 tcgtgagtcc agggccctgg tcaggggaat tgctcatatt tttagccccc acgctctcta
     1561 tgttgtcaca tatccagagc tgagtgccca gggcagactg cacaggatga cagctgtcac
     1621 ccatgcctcc ccagcaacag atcttgctga ggtttcaatt ttgggagctc cagagaggga
     1681 gtttcgcttc ctcatttcag tggccctcag aatttctgct agcttcaggg aaaaactggc
     1741 catgcaggcc tggacagcac aacaagaaat cccagttgtt attcccacct cctactcccg
     1801 tatctataaa aattcagacc tgatcaggga agcgttcttc actgtccaga cacgggttag
     1861 ttgggaaagt tgttgggtga aagctactat ctccaatgca ccaaagacgc cggatgcctg
     1921 cctatggata gatagccacc cgctgtatga ggaaggggcc tcggcctggg gtaaggtgat
     1981 cgactccagg cccccgggtg ggctggttgg ggctgcttcc cagctagtag cccttggaac
     2041 tgacgggcac tgcgtccacc tggccaccac atcggacggg caggcatttt tggtgctgcc
     2101 tgggggcttt gttattaaag gccaattggc actcaccccc gaggagaggg gatatattct
     2161 ggcacgtcat ggcatccgcc gcgaacagcc aactttcttg tacaaagttg gcattataag
     2221 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2281 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2341 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2401 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     2461 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     2521 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     2581 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2641 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     2701 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     2761 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     2821 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     2881 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     2941 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     3001 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     3061 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     3121 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     3181 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     3241 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     3301 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     3361 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     3421 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     3481 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     3541 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     3601 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3661 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3721 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3781 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3841 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3901 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3961 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4021 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4081 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4141 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4201 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4261 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
