LOCUS       sequence-384889-        4224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus SM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-SM
FEATURES             Location/Qualifiers
     source          1..4224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2103..2202)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2219..2238)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2220..2238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2243..2259)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2243..2259)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2690..3481
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3574..4162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4063..4082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gttccttctc agagactctc ccgaactagc agcatttcct ccaacgagga
      721 tcccgcagag agccacattc tggaactcga agcggtctca gacaccaaca cagactgcga
      781 tctggaccct atggagggca gcgaagaaca ctccacagat ggagagattt catcctcaga
      841 ggaggaggat gaagatccaa ctccggccca cgccatacct gcacggccct ccagcgtggt
      901 cataacccct acctcggcat cgtttgtgat tcccagaaag aagtgggacc tacaggacaa
      961 gacagtcaca ttgcatcgct cacccctgtg cagggacgag gacgagaagg aggagactgg
     1021 caactcctct tacaccagag gccacaaaag gcgacgcgga gaggtccatg gctgcaccga
     1081 tgaaagttat ggcaagcgcc gacacctgcc cccgggagca agagcgccca gagccccaag
     1141 ggcccccagg gtgcctagag caccgaggtc tccaagagct ccccgaagca acagagcaac
     1201 cagaggtccc cggtcagaat ctcgaggggc cggcaggagc acaaggaagc aggcgaggca
     1261 agaacgcagc cagaggcccc tgccgaacaa accgtggttt gacatgagtc tggttaagcc
     1321 tgtctccaag attacatttg tcaccttgcc cagccccctg gcctctctga ccctagagcc
     1381 catccaagac ccgttcctac agtcgatgct ggcggtggcc gcccatccag agattggagc
     1441 ctggcagaaa gtgcaaccca gacacgagct gcgcaggagc tacaagacac tacgtgagtt
     1501 tttcaccaag tcaaccaaca aggacacatg gctggatgca cgcatgcagg cgatccagaa
     1561 cgcggggctc tgcaccctgg tggccatgct agaagagacc atcttttggc tccaggagat
     1621 cacctaccac ggcgacctgc ccctagctcc cgcggaagac atcctcctgg cctgcgccat
     1681 gagtcttagc aaggtgatcc tgaccaagct caaagagctg gcaccctgct tccttcctaa
     1741 cacgcgagac tacaactttg tgaagcaact cttctacatc acctgtgcca cggcccgtca
     1801 aaacaaggtg gtggagaccc tgagcagctc atatgtgaag cagcccctct gtctcttggc
     1861 agcatatgcg gcagtagccc cagcctacat taacgccaac tgcagacgga gacacgatga
     1921 agttgaattc ctgggccact acatcaagaa ttacaaccct ggcacgctaa gctccctttt
     1981 gacagaggcc gtggagactc acacacgtga ctgccgaagt gcatcatgca gccgacttgt
     2041 cagggccatt ctctctccgg gcactgggtc actaggactg ttttttgttc ctggattaaa
     2101 tcaaccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2161 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2221 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2281 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     2341 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     2401 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     2461 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2521 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     2581 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2641 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     2701 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2761 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2821 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2881 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2941 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     3001 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     3061 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     3121 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     3181 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     3241 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3301 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3361 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     3421 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     3481 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3541 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3601 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3661 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3721 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3781 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3901 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3961 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4021 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4081 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4141 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4201 ttgctggcct tttgctcaca tgtt
//
