LOCUS       sequence-384900-        4074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BGLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BGLF4
FEATURES             Location/Qualifiers
     source          1..4074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1953..2052)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2069..2088)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2070..2088)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2093..2109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2093..2109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2540..3331
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3424..4012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3913..3932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gatgtgaata tggctgcgga gttgagcccg acgaactcct ccagcagtgg
      721 cgagttgagt gtctccccag aaccccctcg agagacccag gcctttttgg ggaaggtgac
      781 tgtcattgat tacttcacct ttcagcacaa acacctgaag gtgaccaaca ttgatgacat
      841 gacggagacc ctctatgtaa agctgccgga gaacatgacg cgctgtgatc acctccccat
      901 tacctgcgag tatctgctgg ggcgggggag ctacggggcc gtgtatgcac atgcagataa
      961 tgccacggtc aaactctatg actctgtgac ggagctgtat cacgagctca tggtgtgtga
     1021 catgattcag attgggaagg ccacggccga ggatgggcag gacaaggccc tggtggacta
     1081 cctgtcggcc tgcacgtcct gccacgccct gtttatgccc cagttcagat gcagtctcca
     1141 ggattatggc cactggcatg atggtagtat tgagcccctg gtgcggggct ttcagggcct
     1201 caaagatgcc gtttactttc tgaatcggca ctgcggcctc ttccattcgg acattagccc
     1261 cagcaacatc ctggtggatt tcacagacac catgtggggc atgggtaggc tggtcctgac
     1321 tgattatggg actgcttccc tccacgaccg caacaagatg ctggatgtgc ggctaaagtc
     1381 ttctaagggc cggcagctct atcgcctcta ttgccagagg gaaccatttt ctatagccaa
     1441 ggacacctat aagcccctct gccttttgag caagtgctac atcttgaggg gggctgggca
     1501 catccctgac ccctcggcgt gtggccccgt gggggcgcag acggcccttc gcctggatct
     1561 gcagtcgctc ggctactcgc tgctctatgg tatcatgcac ctcgctgact ccacccacaa
     1621 aatcccctac cccaaccctg acatgggatt tgaccgatcc gacccgctct actttttgca
     1681 atttgcagcc ccaaaggtgg tgctgttgga ggtgctgtcg cagatgtgga acctgaactt
     1741 agacatgggc ctgacctcgt gtggcgagag tccgtgcgtg gatgtcacgg cggagcatat
     1801 gagtcaattc ttgcagtggt gccggagcct taaaaagagg ttcaaggaga gctacttctt
     1861 caactgtcgc ccacggtttg agcaccctca tcttccaggt ctggtagctg aactcttggc
     1921 agacgacttc tttggtccag atggccgacg tggaccaact ttcttgtaca aagttggcat
     1981 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2041 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     2101 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     2161 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     2221 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     2281 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     2341 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     2401 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     2461 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2521 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     2581 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     2641 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     2701 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     2761 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     2821 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     2881 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     2941 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     3001 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     3061 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     3121 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     3181 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     3241 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     3301 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     3361 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3541 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
