LOCUS       sequence-384919-       11740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic 
            probe for neuronal inhibition by light or chemical.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Tung JK, Gutekunst CA, Gross RE
  TITLE     Inhibitory luminopsins: genetically-encoded bioluminescent opsins 
            for versatile, scalable, and hardware-independent optogenetic 
            inhibition.
  JOURNAL   Sci Rep. 2015 Sep 24;5:14366. doi: 10.1038/srep14366.
  PUBMED    26399324
REFERENCE   2  (bases 1 to 11740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-09-24"; volume: "5:14366. doi"; pages: " 
            10.1038/srep14366"
COMMENT     Sequence Label: FUiLMO2W
FEATURES             Location/Qualifiers
     source          1..11740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2622..3833
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3670..3689
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4770..4772,4773..5285)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(4812..4833)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5073..5092)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5420..5441
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6432..7020
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6485..6505)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6903..6914)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7023..7039)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7024..7040)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7545..7725
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7751..7768)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7757..7981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8027..8455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8114..8133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8324..8345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8464..8484)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8469..8798
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8649..8784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8711..8730
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8846..8893
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8912..9286
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9416..9537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9453..9472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9507..9526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9586..9602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9586..9602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9599..9621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9610..9626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9634..9640,9641..9658,9659..9664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9679..9700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9817..9834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9988..10576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10068..10087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10747..11538,11539..11607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11370..11389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11608..11712)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3841 gggctgcagg tcgactctag aggatccccg ggtaccggta ggccaccatg acagagaccc
     3901 tgcctcccgt gaccgagagt gccgtggccc ttcaagccga ggttacccaa agggagttgt
     3961 tcgagttcgt gctgaacgac cctttgcttg caagcagtct ctatatcaac atcgcacttg
     4021 caggactgag tatactgctg ttcgttttta tgacccgagg actcgatgat ccacgggcaa
     4081 aacttattgc tgtgtcaacc atccttgtgc ctgtcgtcag cattgcctcc tacactggat
     4141 tggcgagcgg cctgacaatt tccgttcttg aaatgccagc gggccatttt gcagaaggca
     4201 gctcagtgat gctgggagga gaagaggtag atggtgtagt caccatgtgg ggacggtatc
     4261 tcacctgggc actttccacg cccatgattc tcctcgctct gggtctcctg gccggaagca
     4321 atgctacaaa gctcttcaca gctatcactt tcgatatcgc tatgtgcgtg actggccttg
     4381 ccgcggccct gactacctcc tcccacctca tgagatggtt ctggtacgct atcagttgtg
     4441 catgctttct ggtggtcttg tatatcctgc tggtggagtg ggcacaggac gccaaagccg
     4501 cgggaaccgc tgacatgttc aataccctga agctgttgac agtagtgatg tggctggggt
     4561 atccaattgt gtgggctctt ggagtcgagg gtatcgcggt gttgcccgtt ggggtgacga
     4621 gctggggata ttctttcctg gatatcgtgg caaagtacat tttcgcattc ttgctcctga
     4681 actatctgac gtcaaacgaa tctgtcgtgt ccggcagcat tttggatgtt ccatctgctt
     4741 ctgggacccc ggctgatgat gcggccgcag tgagcaaggg cgaggagctg ttcaccgggg
     4801 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4861 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
     4921 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct
     4981 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     5041 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     5101 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     5161 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     5221 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     5281 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5341 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc
     5401 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggggtacca
     5461 aggtgtacga ccccgagcag aggaagagga tgatcaccgg cccccagtgg tgggccaggt
     5521 gcaagcagat gaacgtgctg gacagcttca tcaactacta cgacagcgag aagcacgccg
     5581 agaacgccgt gatcttcctg cacggcaacg ccactagcag ctacctgtgg aggcacgtgg
     5641 tgccccacat cgagcccgtg gccaggtgca tcatccccga tctgatcggc atgggcaaga
     5701 gcggcaagag cggcaacggc agctacaggc tgctggacca ctacaagtac ctgaccgcct
     5761 ggttcgagct tctgaacctg cccaagaaga tcatcttcgt gggccacgac tggggcgccg
     5821 ccctggcctt ccactacgcc tacgagcacc aggacaggat caaggccatc gtgcacatgg
     5881 agagcgtggt ggacgtgatc gagagctggg acgagtggcc agacatcgag gaggacatcg
     5941 ccctgatcaa gagcgaggag ggcgagaaga tggtgctgga gaacaacttc ttcgtggaga
     6001 ccgtgctgcc cagcaagatc atgagaaagc tggagcccga ggagttcgcc gcctacctgg
     6061 agcccttcaa ggagaagggc gaggtgagaa gacccaccct gagctggccc agagagatcc
     6121 ccctggtgaa gggcggcaag cccgacgtgg tgcagatcgt gagaaactac aacgcctacc
     6181 tgagagccag cgacgacctg cccaagctgt tcatcgaggg cgaccccggc ttcttcagca
     6241 acgccatcgt ggagggcgcc aagaagttcc ccaacaccga gttcgtgaag gtgaagggcc
     6301 tgcacttcct ccaggaggac gcccccgacg agatgggcaa gtacatcaag agcttcgtgg
     6361 agagagtgct gaagaacgag cagttctgct acgagaacga ggtgtaagaa ttcgatatca
     6421 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6481 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6541 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6601 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6661 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6721 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6781 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6841 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6901 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6961 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7021 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     7081 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     7141 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7201 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     7261 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     7321 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7381 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     7441 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     7501 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     7561 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7621 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7681 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     7741 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7801 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7861 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7921 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     7981 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     8041 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8101 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8161 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8221 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8281 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8341 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     8401 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     8461 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     8521 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     8581 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     8641 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8701 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     8761 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     8821 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     8881 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     8941 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     9001 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     9061 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     9121 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     9181 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     9241 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     9301 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     9361 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     9421 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9481 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9541 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     9601 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     9661 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     9721 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9781 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    10441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    10621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    10681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    10741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    10801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    10861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    10921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11041 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    11281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    11341 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    11401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    11461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    11521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    11581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    11641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    11701 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
