LOCUS       sequence-38496-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Wang X, Grammatikakis N, Siganou A, Calderwood SK
  TITLE     Regulation of molecular chaperone gene transcription involves the 
            serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic 
            sequestration of heat shock factor 1.
  JOURNAL   Mol Cell Biol. 2003 Sep;23(17):6013-26.
  PUBMED    12917326
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2003-09"; volume: "23(17)"; pages: "6013-26"
COMMENT     Sequence Label: FLAG-HSF1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagcg tttaaactta agcttggtac catggactac aaggacgacg
      121 atgacaagga attcttggat ctgcccgtgg gccccggcgc ggcggggccc agcaacgtcc
      181 cggccttcct gaccaagctg tggaccctcg tgagcgaccc ggacaccgac gcgctcatct
      241 gctggagccc gagcgggaac agcttccacg tgttcgacca gggccagttt gccaaggagg
      301 tgctgcccaa gtacttcaag cacaacaaca tggccagctt cgtgcggcag ctcaacatgt
      361 atggcttccg gaaagtggtc cacatcgagc agggcggcct ggtcaagcca gagagagacg
      421 acacggagtt ccagcaccca tgcttcctgc gtggccagga gcagctcctt gagaacatca
      481 agaggaaagt gaccagtgtg tccaccctga agagtgaaga cataaagatc cgccaggaca
      541 gcgtcaccaa gctgctgacg gacgtgcagc tgatgaaggg gaagcaggag tgcatggact
      601 ccaagctcct ggccatgaag catgagaatg aggctctgtg gcgggaggtg gccagccttc
      661 ggcagaagca tgcccagcaa cagaaagtcg tcaacaagct cattcagttc ctgatctcac
      721 tggtgcagtc aaaccggatc ctgggggtga agagaaagat ccccctgatg ctgaacgaca
      781 gtggctcagc acattccatg cccaagtata gccggcagtt ctccctggag cacgtccacg
      841 gctcgggccc
//
