LOCUS       sequence-384997-        4560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BFRF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BFRF2
FEATURES             Location/Qualifiers
     source          1..4560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2439..2538)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2555..2574)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2556..2574)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2579..2595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2579..2595)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3026..3817
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3910..4498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4399..4418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gcgttattct tggcgcgcca caccttatct ggcacgggtg ctggctgtca
      721 cggccgtggc cctgctcctg atgtttctga ggtggacctg acgttgcagg cccttgggga
      781 gcgggggttc tccaggctcc tggatctggg gctggcctgc ctggatctga gctatgtgga
      841 aatgagggaa tttgtggttt ggggcaggcc cccagcttct gaggcggctg tggcctctac
      901 gccaggctcg cttttccgaa gccactcgtc cgcctactgg ttgtcggagg tggagaggcc
      961 cgggggcctt gtccgctggg ccaggtcaca gaccagcccc tcatccctga ccctcgcgcc
     1021 ccatcttggc ccgtccctct tgtccctttc agtggtcacc ggtggtgggt gtggagccgt
     1081 ggccttttgc aacgcctttt tcctagctta ttttttggtt gtgcggtctg tttttcccgc
     1141 gttttccgat agaatagctg cctggatctg cgcccggtcc cctttctgcg aaaacacccg
     1201 ggccgtggcc aggggttacc gaggcctcgt gaagaggttc ttggcattcg tgtttgagcg
     1261 tagtagctat gaccccccct tgttgaggca aaactctagg cctgtggagc gctgctttgc
     1321 catcaagaat tatgtcccgg gcctggactc acaaagctgt gtgacggtcc cgagcttctc
     1381 ccgctgggcc cagtctcacg ccagcgagct cgatccccgg gagattcgcg acagagttac
     1441 accagcgact gcaccttcgt tcgtggctga tcatgcctcg gctctattgg cctccctcca
     1501 gaagaaggcc tccgacaccc cctgtgggaa tcccattcag tggatgtggt accgcctgtt
     1561 ggtaaactcg tgcctgagga gtgcccactg tcttctgcct atacctgccg tctctgaggg
     1621 ggggagaaag acgggcgggg gcgtagggga ggagctcgtg ggggccgggg ggccctgcct
     1681 gagccgggat gttttcgtgg cgatcgtaag ccgcaatgtt ctctcgtgtc tgctgaacgt
     1741 gcctgccgcg ggtccccggg cctacaagtg tttcagatcc cacgcctcca gaccggtgtc
     1801 tggcccggat taccctccct tggccgtgtt ttgcatggac tgcggttact gcttgaactt
     1861 tggaaagcag acaggtgtag gaggcaggct caattccttt agacccactc tccagtttta
     1921 tccccgtgac cagaaggaga agcatgtgct gacctgccat gccagcggcc gtgtgtactg
     1981 ctccaactgc ggctctgcgg cggtgggctg ccagaggctg gctgagccac cgagcgcccg
     2041 ctcgggctgg cggccccgaa tccgggcagt gctgccgcac aacgcggcct acgagctcga
     2101 ccgtggctcc cgcctcttgg atgccatcat cccctgcttg ggacccgacc gcacttgcat
     2161 gcggccggtg gtcctgcggg gggtgacggt caggcagctc ctgtatttaa ctttgcggac
     2221 agaggccaga gccgtttgct ccatctgtca gcaacgccaa gctccagagg acgcccgcga
     2281 cgagcctcac ctgttctcct cctgtttaga ggtagaattg ccacctggtg agcggtgtgc
     2341 gggctgccgt ctctatcaga cgcgttatgg cacgccggct gcccaagccc accctccagg
     2401 ggaggctgga ggcggatttt ccagacagtc ccctgcttcc ccaactttct tgtacaaagt
     2461 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2521 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2581 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2641 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     2701 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     2761 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     2821 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     2881 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     2941 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     3001 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     3061 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     3121 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     3181 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3241 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3301 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3361 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3421 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3481 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3541 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     3601 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     3661 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     3721 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     3781 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     3841 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3901 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3961 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4021 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4081 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4141 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4201 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4261 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4321 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4381 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4441 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4501 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
