LOCUS       sequence-385013-       13141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Integratable Inducible super-IL2 cytokine + synZiFTR10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Li HS, Israni DV, Gagnon KA, Gan KA, Raymond MH, Sander JD, Roybal 
            KT, Joung JK, Wong WW, Khalil AS
  TITLE     Multidimensional control of therapeutic human cell function with 
            synthetic gene circuits.
  JOURNAL   Science. 2022 Dec 16;378(6625):1227-1234. doi: 
            10.1126/science.ade0156. Epub 2022 Dec 15.
  PUBMED    36520914
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade0156"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1227-1234"
COMMENT     Sequence Label: pHS-393
FEATURES             Location/Qualifiers
     source          1..13141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1002..1067
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1197..1217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1642..1776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1679..1698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1733..1752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1944..2577
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2624..2749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3246..3479
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3664..3708
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3993..4110
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6324..6731
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             7314..7379
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7386..7406
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8256..8282
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8508..8879
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    8948..9536
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9001..9021)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9419..9430)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9539..9555)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9540..9556)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9646..9879
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9904..9922)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9905..9922)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10212..10231)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10445..10464)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10564..10586
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10624..10642)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10710..10814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10815..10883,10884..11675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11033..11052)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11846..12434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12335..12354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12588..12605
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12675..12695)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12680..13009
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12860..12995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
       61 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
      121 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
      181 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
      241 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
      301 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
      361 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
      421 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
      481 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
      541 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
      601 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
      661 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
      721 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
      781 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
      841 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
      901 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
      961 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     1021 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     1081 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     1141 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     1201 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     1261 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     1321 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     1381 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     1441 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     1501 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     1561 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1621 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1681 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1741 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     1801 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     1861 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     1921 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1981 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     2041 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     2101 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     2161 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     2221 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     2281 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     2341 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     2401 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2461 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2521 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2581 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2641 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2701 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2761 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2821 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2881 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     2941 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     3001 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     3061 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     3121 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3181 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3241 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3301 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3361 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3421 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3481 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3541 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     3601 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     3661 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     3721 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     3781 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     3841 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     3901 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     3961 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4021 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4081 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4141 gtttggatcg ataagcttga tatcgaattc ctgcagcctt aggaggcgtt caggtagctc
     4201 atcactctgt cgatggtcac ggccctgatc cggaaggcgt gcagcaggat gcacagcttg
     4261 atcttggtct tgtagaagtc gggttcttcc agggaggact tctggggcac tgtctcgctg
     4321 ttgaagttca gggcctgcat cagctcatcg atcacggcca gcatattctg gtccaggaaa
     4381 atctgccgct tggggtccat cagcagtttg gcgttcatgg tcttgaactc cacctggtac
     4441 atcttcaggt cctcgtagat gctgctcagg cacagggcca tcatgaagga ggtctttctg
     4501 gaggccaggc aagagccgtt ggtgatgaag ctggtttccc ggctgttcag gcagctctcg
     4561 ttcttggtca gttccagggg cagacaggct tccacggtgc tggtcttatc cttggtgatg
     4621 tcctcgtggt cgatttcctc gctggtgcag gggtagaact ccagggtctg tctggccttc
     4681 tgcagcatgt tggacacggc ccgcagcagg ttctggctgt ggtgcagaca aggaaacatg
     4741 ccggggtcag gggtggccac aggcagattt ctggagcctc cgcctccgcc gccagaacat
     4801 ggcacgctgg cccactcgct ccaagagctg ctgtagtacc gatcctgggc ccgcacgcta
     4861 atgctggcgt tcttccggca gatcacggtg gcgctggtct tgtcggtgaa cacccggtcc
     4921 tttttctcgc gcttggactt gccctgcact tgcacacaga atgtcaggct gaagtagctg
     4981 tggggggtgg accaggtgtc ggggtactcc caggacactt ccacctgtct gctgttcttc
     5041 aggggcttca gctgcaggtt cttggggggg tcgggcttga tgatgtcccg gatgaaaaag
     5101 ctgctggtgt agttctcgta cttcagcttg tgcacggcgt ccaccatcac ttcgataggc
     5161 aggctttcct cggcggcagg gcaggcgcta tcttcctggc attccacgct gtactcgtat
     5221 tctttgttgt cgccccgcac tctctcggca gacagtgtag cggcgccaca tgtcacgccc
     5281 tgagggtcgc tgctgcctct gctggacttc acgctgaagg tcaggtcggt gctgatggtg
     5341 gtcagccacc aacaggtgaa ccggccgctg tagttcttgg cctcgcatct caggaaggtc
     5401 ttgttcttgg gctctttctg gtccttcagg atgtcggtgc tccaaatgcc atcctctttc
     5461 ttgtgcagca gcagcaagct gtggctcagc acctctccgc ccttgtgaca ggtgtactgg
     5521 ccggcgtcgc cgaactcttt cacttggatg gtcagggtct tgccgctgcc cagcacctcg
     5581 ctgctctgat ccagggtcca ggtgatgccg tcctcttcgg gggtatcgca ggtcagcacc
     5641 accatctcgc caggggcgtc gggataccag tccagttcca ccacgtacac gtctttcttc
     5701 agctcccaga tggccaccag agggctggcc aggaacacca ggctgaacca gctgatcacg
     5761 agctgctgat ggcacatggt ggcgatccgg tagcgctagc gatgagctct ggtagtagac
     5821 tagtggcccc cattatatac cctctagagg atcctatcca ctccgtgtaa gggagagtga
     5881 gcctcttacg aatgcgcgac atcggctacg ccgttccgag gcgactgata cgcgcgacat
     5941 cggctacgcc gtacgaaggc agtccgattg cgcgacatcg gctacgccga cctttactga
     6001 gacgggagcg cgacatcggc tacgccgaag gcgttgcgaa tcctcatgcg attgttacga
     6061 aacccggcgc gacatcggct acgccgtatt ggaagcacgg ctctgcgcga catcggctac
     6121 gccgacctcg ggagaactga ttgcgcgaca tcggctacgc cgaagaggtt gacgctatcc
     6181 gcgcgacatc ggctacgccg agccctgtag gtagactaga tacagtgtta ctcaatcgtc
     6241 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     6301 gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac
     6361 caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa
     6421 caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg
     6481 cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca
     6541 gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg
     6601 cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc
     6661 ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg
     6721 agtcgcccgg gggggatctg gagctctcga gaattctcac gcgtgccgcc accatgtcta
     6781 gacccggaga gcgcccattc cagtgtcgga tttgcatgcg gaacttttcg agaagacacg
     6841 gcctggacag acatacccgt actcatacag gtgaaaaacc ctttcagtgt cggatctgta
     6901 tgcgaaattt ctccgaccac agcagcctga agagacatct acgtacccac accggcagcc
     6961 agaagccatt tcagtgtcgg atctgtatgc ggaacttctc cgtgagacac aacctgacca
     7021 gacatctacg tacgcacacc ggagagaagc cattccaatg ccgaatatgc atgcgcaact
     7081 tcagtgacca cagcaacctg agcagacacc taaaaaccca caccggttcc cagaagccat
     7141 ttcagtgtcg gatctgtatg cggaacttct cccagcgcag cagcctggtg agacatctac
     7201 gtacgcacac cggagagaag ccattccaat gccgaatatg catgcgcaac ttcagtgaga
     7261 gcggccacct gaagagacac ctgcgtacgc acctgagggg atccacctgc agggactaca
     7321 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaaga
     7381 tggcccccaa gaaaaagagg aaggtgggca ttcacggggt gccgggtgga ctcgagggag
     7441 gcggtggaag cggcggtacc gaggacgtgg tgtgctgcca ctcaatctac ggcaagaaga
     7501 agggtgatat cgacacctac cgatacatag gctcttccgg gacaggctgc gtggtcatag
     7561 tgggcaggat cgtcttgtcc ggatccggca ctagtgcgcc catcacggcg tacgcccagc
     7621 agacgagagg cctcctaggg tgtataatca ccagcctgac tggccgggac aaaaaccaag
     7681 tggagggtga ggtccagatc gtgtcaactg ctacccaaac cttcctggca acgtgcatca
     7741 atggggtatg ctgggcagtc taccacgggg ccggaacgag gaccatcgca tcacccaagg
     7801 gtcctgtcat ccagatgtat accaatgtgg accaagacct tgtgggctgg cccgctcctc
     7861 aaggttcccg ctcattgaca ccctgtacct gcggctcctc ggacctttac ctggtcacga
     7921 ggcacgccga tgtcattccc gtgcgccggc gaggtgatag caggggtagc ctgctttcgc
     7981 cccggcccat ttcctacttg aaaggctcct cggggggtcc gctgttgtgc cccgcgggac
     8041 acgccgtggg cctattcagg gccgcggtgt gcacccgtgg agtggctaaa gcggtggact
     8101 ttatccctgt ggagaaccta gagacaacca tgagatcccc ggtgttcacg gacaactcct
     8161 ctccaccagc agtcaccctg acgcacccaa tcaccaaaat cgatagggag gttctctacc
     8221 aggagttcga tgagatggaa gagtgctctc agcactatcc ctacgatgtg cccgattacg
     8281 ctggaggcgg tggaagcggc ggtaccgatg agtttcccac catggtgttt ccttctgggc
     8341 agatcagcca ggcctcggcc ttggccccgg cccctcccca agtcctgccc caggctccag
     8401 cccctgcccc tgctccagcc atggtatcag ctctggccca ggccccagcc cctgtcccag
     8461 tcctagcccc aggccctcct caggctgtgg ccccacctgc ccccaagccc acccaggctg
     8521 gggaaggaac gctgtcagag gccctgctgc agctgcagtt tgatgatgaa gacctggggg
     8581 ccttgcttgg caacagcaca gacccagctg tgttcacaga cctggcatcc gtcgacaact
     8641 ccgagtttca gcagctgctg aaccagggca tacctgtggc cccccacaca actgagccca
     8701 tgctgatgga gtaccctgag gctataactc gcctagtgac aggggcccag aggccccccg
     8761 acccagctcc tgctccactg ggggccccgg ggctccccaa tggcctcctt tcaggagatg
     8821 aagacttctc ctccattgcg gacatggact tctcagccct gctgagtcag atcagctccc
     8881 aattgtaagc ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
     8941 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     9001 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     9061 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     9121 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     9181 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     9241 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     9301 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     9361 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     9421 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     9481 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     9541 ataccgtcga cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca
     9601 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     9661 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     9721 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9781 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9841 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     9901 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     9961 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
    10021 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
    10081 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
    10141 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
    10201 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
    10261 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
    10321 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
    10381 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
    10441 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
    10501 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    10561 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
    10621 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    10681 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10741 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10801 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10861 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10921 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10981 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    11041 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    11101 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    11161 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    11221 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    11281 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    11341 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    11401 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    11461 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    11521 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    11581 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    11641 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    11701 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11761 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11821 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11881 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11941 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    12001 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    12061 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    12121 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    12181 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    12241 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    12301 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12361 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12421 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12481 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    12541 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    12601 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    12661 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    12721 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    12781 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    12841 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    12901 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    12961 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
    13021 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    13081 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    13141 a
//
