LOCUS       sequence-385014-        4194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus LF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-LF1
FEATURES             Location/Qualifiers
     source          1..4194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2073..2172)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2189..2208)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2190..2208)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2213..2229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2213..2229)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2660..3451
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3544..4132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4033..4052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gccctgcaaa ctgacacaca ggcctggcgg gtggagatag ggaccagagg
      721 actaatgttc tctaactgtg tcccactcca cctgccggag ggacagtacc acaagctccg
      781 gctgcccgtc tcggcctacg aggccctggc cgtggccagg tacgggctgg tgggctccct
      841 ctgggaggtg cccgccgtga actctgccct tcagtgtctg gcggctgctg cgccctgtaa
      901 agatgtaaag atctacccca gctgcatctt ccaggtgcac gctcccatgt ttgtaactat
      961 aaagacaagc ctgcgttgcc taaatcctca tgacctgtgt ctgtgtctca tttgtgtagg
     1021 tgcggctatc ctagatatac ccctcctatg cgccccccgt gacggagctg gggcacgggc
     1081 cgccgagggg caggctgcgg ccgcccaggg gggcaaattg cgtgtctggg gccgcctgag
     1141 cccatcctcg cccacatcgc tctccctggc ttttccctat gcgggcccgc cccccgtggc
     1201 gtggtaccga cattctatca acctaacaag gagtgaaggt gtggggatcg gcaaagattg
     1261 cgcccaggat cacgcctgcc ccgtcccacc ccaaggtcat gcttcctctg cggctgacca
     1321 ggctggggtg ccggagagag gtagaaagcg ggcccatgaa ggcccggggg ctggcgaggc
     1381 ggcgtccgcg ggccgagggg atgtggccct gagtcagtcc cgggctctgc tctggcgcgg
     1441 cctcggctgg gacacgggac gtggtcggct ggcaccaggc ctggccatgt cacgagacgc
     1501 agcctccggg tccgtgcacc tggacataca ggtggacagg gccgaggagg gctgggtctg
     1561 cgacgttctg ctggagcccg ggccgcccac cgcccgggag ggctgttttc taagcatgga
     1621 ccccggtctc gtgaccctga aagatgcctg gacgctattc cccctccacc cggagcacga
     1681 cgccgtcgtt ccacccaagg aggaaataca tgtgatggca cagggccatc tccagggcgg
     1741 cacgccctct ctctgggggt ttacattcca ggaggcggcg tgcgaccaat gggtgctgcg
     1801 cccgcgcgtg tggaccgccc actcgcccat aaaaatgaca gtctacaact gcgggcacaa
     1861 accgctgcac atcggcccca gcaccagact tggacttgcg ctcttctggc cggccgagcg
     1921 aagtgacaac ctagacgccg ggcggatatt ctaccagcta acgagcggag agttgtattg
     1981 gggccgcacc gtcgcccgcc ccccgactct caccttgccc gtcgacgagc tccggccatg
     2041 gccgaagctt accacggagg agcccatgca gcacccaact ttcttgtaca aagttggcat
     2101 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2161 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     2221 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     2281 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     2341 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     2401 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     2461 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     2521 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     2581 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2641 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     2701 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     2761 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     2821 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     2881 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     2941 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     3001 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     3061 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     3121 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     3181 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     3241 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     3301 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     3361 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     3421 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     3481 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3661 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4141 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
