LOCUS       sequence-385016-        9292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Editing human TSC2 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, 
            Durnberger G, Mechtler K, Knoblich JA
  TITLE     Integrated transcriptome and proteome analysis reveals 
            posttranscriptional regulation of ribosomal genes in human brain 
            organoids.
  JOURNAL   Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135.
  PUBMED    36989136
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-03-29"; volume: "12:e85135. doi"; pages: " 
            10.7554/eLife.85135"
COMMENT     Sequence Label: pSpCas9(BB)-2A-tomato-TSC2-sgRNA
FEATURES             Location/Qualifiers
     source          1..9292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(99..118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      912..1500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1401..1420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1562..1810
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1562..1582
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1733..1752
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1831..1906
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        2003..2288
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          2567..2794
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      2806..2815
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2815..2880
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2887..2907
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2932..5469,5470..5472,5473..5934,5935..5937,5938..6105,
                     6106..6108,6109..7032)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7033..7080
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7096..7152
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7159..7860
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(7292..7310)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7754..7777
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7894..7911)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7900..8107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8116..8256
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8116..8245
                     /label=AAV2 ITR
     rep_origin      8331..8786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8418..8437)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8628..8649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8803..8822)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8922..8944
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8982..9000)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9068..9172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9173..9241,9242..9292,1..741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
       61 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      121 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      181 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      241 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      301 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      361 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      421 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      481 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      541 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc
      601 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      661 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      721 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      781 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      841 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
      901 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      961 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1021 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1081 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1141 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1201 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1261 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1321 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1381 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1441 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1501 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1561 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     1621 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     1681 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     1741 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     1801 acgaaacacc gctttagggc gagcgtttgg gttttagagc tagaaatagc aagttaaaat
     1861 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
     1921 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
     1981 gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac
     2041 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac
     2101 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     2161 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc
     2221 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     2281 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     2341 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     2401 gggggggggg sgcgcgccrg gsggggsggg gsggggsggg gggsggggsg gggsgrggsg
     2461 grgrggkgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     2521 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     2581 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     2641 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     2701 gctgagcaag aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta
     2761 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catggactat
     2821 aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga tgacgataag
     2881 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag
     2941 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     3001 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     3061 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     3121 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     3181 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     3241 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
     3301 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
     3361 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
     3421 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     3481 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     3541 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
     3601 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
     3661 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     3721 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     3781 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     3841 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     3901 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     3961 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     4021 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     4081 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
     4141 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     4201 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     4261 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     4321 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     4381 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     4441 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     4501 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
     4561 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
     4621 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
     4681 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
     4741 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
     4801 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
     4861 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
     4921 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
     4981 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
     5041 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
     5101 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
     5161 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
     5221 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     5281 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
     5341 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     5401 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
     5461 agctttctgg cggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
     5521 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     5581 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     5641 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     5701 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     5761 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     5821 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     5881 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctgcgctg
     5941 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     6001 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     6061 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaaggcgcc tctgatcgag
     6121 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
     6181 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
     6241 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag
     6301 gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg
     6361 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
     6421 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
     6481 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
     6541 gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac
     6601 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
     6661 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
     6721 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
     6781 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
     6841 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
     6901 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
     6961 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
     7021 ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag
     7081 ggatccggaa gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag
     7141 aaccctggac cttacgtaat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc
     7201 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     7261 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     7321 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     7381 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     7441 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     7501 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     7561 cccgtaatgc agaagaaaac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     7621 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     7681 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     7741 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     7801 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
     7861 tgataagaat tctaactaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     7921 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7981 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     8041 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
     8101 ctggggagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     8161 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     8221 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     8281 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     8341 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8401 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8461 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8521 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     8581 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8641 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     8701 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8761 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     8821 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     8881 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     8941 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     9001 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     9061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg ca
//
