LOCUS       sequence-38503-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Ahn S, Kim J, Lucaveche CL, Reedy MC, Luttrell LM, Lefkowitz RJ, 
            Daaka Y
  TITLE     Src-dependent tyrosine phosphorylation regulates dynamin 
            self-assembly and ligand-induced endocytosis of the epidermal growth
            factor receptor.
  JOURNAL   J Biol Chem. 2002 Jul 19;277(29):26642-51. Epub 2002 May 13.
  PUBMED    12011079
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-07-19"; volume: "277(29)"; pages: "26642-51. 
            Epub 2002 May 13"
COMMENT     Sequence Label: Dynamin-I-K44A-6His/Flag
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             173..196
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg
      121 aattctgcag atctagtgga tcgccaccat gcaccatcat caccatcacg ctgactacaa
      181 agacgatgac gacaaggaat tcgggatcct cgagaccatg ggcaaccgcg gcatggaaga
      241 cctcatcccg ctggttaacc ggttacagga cgccttctct gccatcggcc agaacgcgga
      301 cctcgacttg ccgcagatcg ccgtggtagg cggccagagc gccggcgcca gctcggtgct
      361 ggagaatttc gtgggcaggg acttcttgcc ccgaggatcc ggcatcgtca cccggcgtcc
      421 cctggtcctg cagctggtta attctaccac agaatatgcc gagttcctgc actgcaaggg
      481 gaagaaattc accgacttcg aggaggtgcg cctggagatc gaggctgaga ccgaccgagt
      541 cactggcacc aacaagggca tttcgcctgt gcccatcaac ctgcgggtct actcacccca
      601 tgtgctcaac ctgactctag tggacctgcc aggaatgacc aaggtcccag ttggggacca
      661 acctcctgac atcgagttcc agatccggga catgcttatg cagtttgtca ctaaggagaa
      721 ctgccttatc ctggctgtgt cccctgccaa ctcggacctg gccaactctg atgccctcaa
      781 gatcgccaag gaggtggacc cccagggtca gcgcaccatt ggggtcatca ccaagttgga
      841 cctgatggac gagggcacag atgctcggga tgtgctggag aacaa
//
