LOCUS       sequence-38505-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pNN_v2
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(554..571)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(554..570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..686)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagcaggtcg tggcaattgc gagcaacaac gggggaaagc aggcactcga aaccgtccag
       61 aggttgctgc ctgtgctgtg ccaagcgcac ggacgtcaaa agggcgacac aaaatttatt
      121 ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt gtattatcgt
      181 tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc gagatttatt
      241 ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat aaataataga
      301 tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta tttaaagtgc
      361 gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa agtgacaggc
      421 gcccttaaat attctgacaa atgctctttc cctaaactcc ccccataaaa aaacccgccg
      481 aagcgggttt ttacgttatt tgcggattaa cgattactcg ttatcagaac cgcccagggg
      541 gcccgagctt aagactggcc gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa
      601 aggccatccg tcaggggcct tctgcttagt ttgatgcctg gcagttccct actctcgcct
      661 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      721 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataccgc aggaaagaac
      781 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa a
//
