LOCUS       sequence-38506-e        2286 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Lin CW, Ting AY
  TITLE     A genetically encoded fluorescent reporter of histone 
            phosphorylation in living cells.
  JOURNAL   Angew Chem Int Ed Engl. 2004 May 24;43(22):2940-3.
  PUBMED    15170310
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl."; date: "2004-05-24"; volume: "43(22)"; pages: "2940-3"
COMMENT     Sequence Label: pcDNA3-S28 histone 3 phosphorylation reporter-NLS
FEATURES             Location/Qualifiers
     source          1..2286
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(13..15,16..18,19..531)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             join(1528..1530,1531..1533,1534..2244)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2245..2265
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gataaggatc cgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      301 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      421 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      481 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcagc
      541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      601 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
      661 gatcacatgg tcctgctgga gttcgtgacc gccgcccgca tgcatgatat catggagaag
      721 actgagctga tccagaaggc caagctggcc gagcaggccg agcgctacga cgacatggcc
      781 acctgcatga aggcagtgac cgagcagggc gccgagctgt ccaacgagga gcgcaacctg
      841 ctctccgtgg cctacaagaa cgtggtcggg ggccgcaggt ccgcctggag ggtcatctct
      901 agcatcgagc agaagaccga cacctccgac aagaagttgc agctgattaa ggactatcgg
      961 gagaaagtgg agtccgagct gagatccatc tgcaccacgg tgctggaatt gttggataaa
     1021 tatttaatag ccaatgcaac taatccagag agtaaggtct tctatctgaa aatgaagggt
     1081 gattacttcc ggtaccttgc tgaagttgcg tgtggtgatg atcgaaaaca aacgatagat
     1141 aattcccaag gagcttacca agaggcattt gatataagca agaaagagat gcaacccaca
     1201 cacccaatcc gcctggggct tgctcttaac ttttctgtat tttactatga gattcttaat
     1261 aacccagagc ttgcctgcac gctggctaaa acggcttttg atgaggccat tgctgaactt
     1321 gatacactga atgaagactc atacaaagac agcaccctca tcatgcagtt gcttagagac
     1381 aacctaacac tttggacatc agactctgca ggcggcaccg gcggcagcct cgcccggacg
     1441 aagcagacag ctcgcaagtc taccggcggc aaggcaccgc ggaagcagct ggccaccaag
     1501 gcagcgcgca aaagcgctcc agagctcatg gtgagcaagg gcgaggagct gttcaccggg
     1561 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1621 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1681 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc
     1741 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1801 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1861 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1921 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1981 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2041 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2101 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     2161 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2221 ctcggcatgg acgagctgta caagccaaaa aagaagagaa aggtggaaga cgcttaagaa
     2281 ttccgg
//
