LOCUS       sequence-385060-        9293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Editing human SOX2 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, 
            Durnberger G, Mechtler K, Knoblich JA
  TITLE     Integrated transcriptome and proteome analysis reveals 
            posttranscriptional regulation of ribosomal genes in human brain 
            organoids.
  JOURNAL   Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135.
  PUBMED    36989136
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-03-29"; volume: "12:e85135. doi"; pages: " 
            10.7554/eLife.85135"
COMMENT     Sequence Label: pSpCas9(BB)-2A-tomato SOX2-sgRNA
FEATURES             Location/Qualifiers
     source          1..9293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      173..761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     662..681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        823..1071
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     823..843
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     994..1013
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1093..1168
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        1265..1550
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          1829..2056
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      2068..2077
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2077..2142
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2149..2169
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2194..4731,4732..4734,4735..5196,5197..5199,5200..5367,
                     5368..5370,5371..6294)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6295..6342
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6358..6414
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6421..7122
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(6554..6572)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7016..7039
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7156..7173)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7162..7369
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7378..7518
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7378..7507
                     /label=AAV2 ITR
     rep_origin      7593..8048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7680..7699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7890..7911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8065..8084)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8184..8206
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8244..8262)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8330..8434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8435..8503,8504..9293,1..2)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8653..8672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
       61 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      121 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      181 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      241 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      301 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      361 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      421 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      481 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      541 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      601 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      661 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      721 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      781 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtgagggcct atttcccatg
      841 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
      901 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
      961 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     1021 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagcggcc
     1081 cggtgcccgg cagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     1141 acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta gagctagaaa tagcaagtta
     1201 aaataaggct agtccgtttt tagcgcgtgc gccaattctg cagacaaatg gctctagagg
     1261 tacccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1321 cattgacgtc aatagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1381 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1441 ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg accttatggg
     1501 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     1561 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     1621 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggcgcgcgcc
     1681 rggsggggsg ggggggggsg gggggsgggg sggggsgrgg sggrgrggkg cggcggcagc
     1741 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     1801 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     1861 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1921 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagctgagca agaggtaagg
     1981 gtttaaggga tggttggttg gtggggtatt aatgtttaat tacctggagc acctgcctga
     2041 aatcactttt tttcaggttg gaccggtgcc accatggact ataaggacca cgacggagac
     2101 tacaaggatc atgatattga ttacaaagac gatgacgata agatggcccc aaagaagaag
     2161 cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat cggcctggac
     2221 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
     2281 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
     2341 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
     2401 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
     2461 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
     2521 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
     2581 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
     2641 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
     2701 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
     2761 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc
     2821 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc
     2881 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc
     2941 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc
     3001 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt
     3061 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc
     3121 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag
     3181 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt
     3241 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa
     3301 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc
     3361 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc
     3421 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac
     3481 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac
     3541 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag
     3601 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc
     3661 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag
     3721 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg
     3781 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac
     3841 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag
     3901 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc
     3961 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag
     4021 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag
     4081 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag
     4141 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc
     4201 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac
     4261 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa
     4321 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc
     4381 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg
     4441 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc
     4501 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag
     4561 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag
     4621 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc
     4681 aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct ggcggacgac
     4741 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg
     4801 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag
     4861 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa
     4921 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac
     4981 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc
     5041 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc
     5101 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
     5161 gccgtcgtgg gaaccgccct gatcaaaaag taccctgcgc tggaaagcga gttcgtgtac
     5221 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
     5281 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
     5341 accctggcca acggcgagat ccggaaggcg cctctgatcg agacaaacgg cgaaaccggg
     5401 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
     5461 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
     5521 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
     5581 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
     5641 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
     5701 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
     5761 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
     5821 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc
     5881 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
     5941 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
     6001 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
     6061 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
     6121 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc
     6181 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag
     6241 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg cgacaaaagg
     6301 ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccgg aagcggagct
     6361 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg accttacgta
     6421 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     6481 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6541 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     6601 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     6661 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6721 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     6781 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaaa
     6841 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     6901 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     6961 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     7021 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     7081 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtgataaga attctaacta
     7141 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     7201 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     7261 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     7321 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggag cggccgcagg
     7381 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     7441 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     7501 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     7561 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     7621 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     7681 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7741 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7801 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7861 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7921 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     7981 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     8041 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     8101 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     8161 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     8221 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     8281 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8341 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8401 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8461 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8521 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8581 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8641 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8701 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8761 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8821 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8881 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8941 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     9001 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     9061 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9121 tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag cactggggcc
     9181 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     9241 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggt
//
