LOCUS       sequence-38508-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: JDS70
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..52)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(68..655)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaagg
       61 gcccttaaaa gtttatctcg ccgttattaa atttccgtct gacttcctct aaggttaatg
      121 tgccggcttt aatcatttct ccaccaatta aaagctcttc tacactaaga acagctccat
      181 tacaattagt gatatgattt aatcgtgtaa gctgagcttt gtagtttcct ttaaagtgac
      241 cactcacaaa taaaaactta aattccgtta cagaagatgg atagactttc caccattcat
      301 tagggttgat atgtttgttt cgtgtttgat tttcttcgac atatcgttgc atttcatctg
      361 cttggccaat tggcagatta taacctccgc tataagcttt agtatccacg atcacaccgt
      421 aatcaatagg agatccgaca gtataaattg ctccgtccgg tttccttgat ccacccaaat
      481 gtttacctct atatccataa actttcataa aaaattccat taccttcatt tcaagaattc
      541 tatcctgagt ggaatttctg gcaatttcaa ttaattcaat atattcatga ggcacatatt
      601 tcaatttatg acgaagttca gatttcttct cctccagttc acttttgact agttgggatc
      661 ccgcgactcg atgggaagtt ctctcgggaa tccgccggtt ggttcttttg atcaatgcgg
      721 gagcatgagg cagacccttt ttgactgcat cgagcgcggg tcgtccacca agacatgcca
      781 acgccaccag atggtcattc gttaacgcag ccaacgcggg atcgggcctg gacaattggg
      841 ctacgatgga ctccaaggct ggccgtcctc cgatgttaga agcaatggcg accacctgtt
      901 cgggtgtcag cgagac
//
