LOCUS       sequence-385149-        4002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BBRF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BBRF3
FEATURES             Location/Qualifiers
     source          1..4002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1881..1980)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1997..2016)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1998..2016)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2021..2037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2021..2037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2468..3259
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3352..3940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3841..3860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg aagtcctcca agaatgacac gttcgtctat agaacgtggg tcaaaacgct
      721 tgttgtgtac tttgtgatgt ttgtcatgtc ggcggtggtc cccatcaccg ccatgttccc
      781 caacctgggg tacccctgct actttaacgc actggttgat tacggggcac ttaacctgac
      841 caattacaac ctggcccacc acctgacccc cacgctctat ctggagccgc cggagatgtt
      901 tgtctacatc acactggtct ttatcgcgga ctgcgtggct ttcatctact acgcctgcgg
      961 cgaggtggcg ctaatcaagg cccgaaaaaa ggtctcgggt cttacagacc tctcggcctg
     1021 ggtctcggca gtgggctccc caaccgtgct gtttttggcc atcctcaagc tctggtccat
     1081 acaggtcttc atccaggtcc tttcctacaa gcacgtcttt ctctcggcct ttgtgtactt
     1141 tttgcacttt ctggcctcag ttctacacgc ctgcgcatgt gtgacccgct tctccccggt
     1201 ctgggtggtc aaggcccagg acaactctat tccccaggac accttcttgt ggtgggtggt
     1261 cttctacctg aagcccgtag ttacaaacct gtacctgggg tgccttgccc tggagacgct
     1321 ggtcttctcg ctcagcgtgt tcctggccct gggcaacagc ttttacttta tggtggggga
     1381 catggtgctg ggagccgtga acctcttcct catcctgccc atcttctggt acattctgac
     1441 ggaggtgtgg ctggcctcct tcctgcggca caactttggc ttctactgcg gcatgttcat
     1501 cgcctccatc atcctgatcc tgcccttggt caggtacgag gccgtctttg tctccgccaa
     1561 gctgcacacc actgtggcca tcaatgtggc catcatacct atcctgtgct cggtggccat
     1621 gctcatcagg atatgccgga ttttcaaaag catgcgccag ggcactgact atgtccctgt
     1681 ctcggagacg gtggaactgg agctagagtc agagccgagg cctaggccct cgcgcacgcc
     1741 atcacccggg cgcaaccgcc gccgctcttc tacgtcctca tcttcctcca ggtcaaccag
     1801 gagacagagg cccgtctcta cccaagccct cgtctcctcc gttttaccga tgacgacgga
     1861 cagcgaggag gagatcttcc ccccaacttt cttgtacaaa gttggcatta taagaaagca
     1921 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     1981 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2041 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2101 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2161 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2221 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2281 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2341 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2401 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2461 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2521 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2581 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     2641 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     2701 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     2761 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     2821 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     2881 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     2941 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3001 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3061 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3121 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3181 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3241 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3301 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3361 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3421 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3481 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3541 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3601 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3661 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3721 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3781 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3841 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3901 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3961 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
