LOCUS       sequence-385211-        8187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human wild-type short-form UBASH3A with 
            GFP(11) tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8187)
  TITLE     Concannon Lab Plasmids
REFERENCE   2  (bases 1 to 8187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WT-short-UBASH3A-GFP11
FEATURES             Location/Qualifiers
     source          1..8187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(326..1117,1118..1186))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     949..968
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1187..1291)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1359..1377
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             1496..1729
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1804..2392
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1910..1921
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2319..2339
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2406..3119)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(2409..3119)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(join(3153..3863,3864..3866,3867..3869))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3153..3863,3864..3866,3867..3869))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3195..3216)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3544..3563
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3803..3824
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3866..3875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4608..4631)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(4632..4658)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(4632..4658)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(4662..4682)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4685..4694
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4702..5100)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     misc_feature    5143..5260
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5553..5597)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5782..6015
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6512..6637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6684..7317
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7509..7528)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7578..7594)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7578..7594)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7591..7613)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7602..7618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7626..7632,7633..7650,7651..7656))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7671..7692
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7932..7949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
       61 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
      301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
      361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
      421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
      481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
      541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
      601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
      661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
      721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
      781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
      841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
      901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
      961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     1141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     1201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     1261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     1321 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     1381 ttcgtcttca agaatgatct agccctttcc ttaattaacc cgggcctctc actctctgat
     1441 attcatttct ttgcaagtta taaatactga ataataagat gacatgaact actactgcta
     1501 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     1561 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     1621 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     1681 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
     1741 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggatct
     1801 caggcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg
     1861 gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
     1921 aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
     1981 aaggaaagga cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag
     2041 cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg
     2101 ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg
     2161 ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct
     2221 cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag
     2281 caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat
     2341 agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttaggatcta
     2401 tcgatttatt tgtacaattc atccatacca tgggtaatac cagcagcagt aacaaattct
     2461 aacaagacca tgtggtctct cttttcgttt ggatctttgg ataaggcaga ttgagtggat
     2521 aagtaatggt tgtctggtaa caagactgga ccatcaccaa ttggagtatt ttgttgataa
     2581 tggtcagcta attgaacaga accatcttca atgttgtgtc taattttgaa gttaactttg
     2641 ataccattct tttgtttgtc agccatgatg taaacattgt gagagttata gttgtattcc
     2701 aatttgtgac ctaaaatgtt accatcttct ttaaaatcaa taccttttaa ttcgattcta
     2761 ttaactaagg tatcaccttc aaacttgact tcagctctgg tcttgtagtt accgtcatct
     2821 ttgaaaaaaa tagttctttc ttgaacataa ccttctggca tggcagactt gaaaaagtca
     2881 tgttgtttca tatgatctgg gtatctagaa aaacattgaa caccataagt taaagtagtg
     2941 actaaggttg gccatggaac tggcaattta ccagtagtac aaataaattt taaggtcaat
     3001 ttaccgtaag tagcatcacc ttcaccttca ccggagacag aaaatttgtg accattaaca
     3061 tcaccatcta attcaaccaa aattgggaca acaccagtga ataattcttc acctttagag
     3121 cctgatgaac ctgaaccgca tgcacctgtg cccttgtaca gctcgtccat gccgagagtg
     3181 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     3241 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     3301 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     3361 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     3421 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     3481 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     3541 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     3601 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     3661 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     3721 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
     3781 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     3841 gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtgggtcggc gtagatgccg
     3901 ctgttggcgg cgatggcgct ggggatgggg ttgccgtcct tcagcaggcc ctgcatggcc
     3961 ttcacgatca gctcgcagtc gctgttggtg gcgaagatgg ggatggtcag ctccatgttc
     4021 aggtagcttc tccaggcgcc cttgggcacc tccaccttga tggtgtactt tctgttctgg
     4081 gcgctgctct gtctcacgct gcaggtcacc ttgtaggcct ggcttctgct gttgctgctg
     4141 atccactcgg cgatgccgtt ggcgaagttg ctgggggcca cggtcacgtc gccggtgccg
     4201 ccgttgtcca ccagcacgaa ctgggtgaag ttgctggcca tggcgtagat gccggagttt
     4261 gctgcgattg ctgagggaat cgggtttcca tcttttagga gaccttgcat tgccttaaca
     4321 ataagctcgc agtcggaatt cgtggcgaaa attggaatgg ttagttccat atttaagtac
     4381 gaacgccagg cgcctttagg cacctcgact ttgatggtgt atttgcgatt ctgcgcagag
     4441 ctctgacgaa cgctacaggt tactttgtaa gcctgtgaac gcgagttaga gctgatccat
     4501 tcagcgatcc cgttagcgaa gttgcttggg gcgacagtca cgtcgccagt tccgccattg
     4561 tcgacgagaa cgaactgagt aaagttagaa gcgttaatta ataacagggg cccctggaac
     4621 agaacttcca gggcgtagtc gggcacgtcg taggggtagc caacttttct tttctttttt
     4681 gggcccatgg tggcgcggcc gccaagtgac gatcacagcg atccacaaac aagaaccgcg
     4741 acccaaatcc cggctgcgac ggaactagct gtgccacacc cggcgcgtcc ttatataatc
     4801 atcggcgttc accgccccac ggagatccct ccgcagaatc gccgagaagg gactactttt
     4861 cctcgcctgt tccgctctct ggaaagaaaa ccagtgccct agagtcaccc aagtcccgtc
     4921 ctaaaatgtc cttctgctga tactggggtt ctaaggccga gtcttatgag cagcgggccg
     4981 ctgtcctgag cgtccgggcg gaaggatcag gacgctcgct gcgcccttcg tctgacgtgg
     5041 cagcgctcgc cgtgaggagg ggggcgcccg cgggaggcgc caaaacccgg cgcggaggcc
     5101 actagtccaa actggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
     5161 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     5221 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
     5281 ttgtgaattc ggcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct
     5341 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
     5401 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     5461 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     5521 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     5581 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     5641 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     5701 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     5761 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     5821 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     5881 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     5941 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     6001 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     6061 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     6121 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct
     6181 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
     6241 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
     6301 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     6361 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     6421 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     6481 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     6541 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     6601 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     6661 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     6721 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     6781 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     6841 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     6901 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     6961 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     7021 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     7081 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     7141 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     7201 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     7261 ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttccaact
     7321 actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag
     7381 gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat agggtatggg
     7441 gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca gacatgataa
     7501 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     7561 gtgaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7621 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7681 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7741 cacggggagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     7801 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     7861 agcttgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7921 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7981 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8041 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8101 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8161 tggcgaaacc cgacaggact ataaaga
//
