LOCUS       sequence-385247-        8944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Blelloch R, Venere M, Yen J, Ramalho-Santos M
  TITLE     Generation of induced pluripotent stem cells in the absence of drug 
            selection.
  JOURNAL   Cell Stem Cell. 2007 Sep 13;1(3):245-7. doi: 
            10.1016/j.stem.2007.08.008. Epub 2007 Sep 6.
  PUBMED    18371358
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2007.08.008"; journalName: "Cell Stem Cell"; date: 
            "2007-09-13- 13"; volume: "1"; issue: "3"; pages: "245-7"
COMMENT     Sequence Label: pLOVE-Sox2
FEATURES             Location/Qualifiers
     source          1..8944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..614
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1624..1648)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1701..1742
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(1780..1813)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1869..2457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1922..1942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2340..2351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2460..2476)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2461..2477)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2986..3166
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(3228..3816)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3308..3327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3987..4778,4779..4847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4610..4629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4848..4952)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5027..5046)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5218..5597
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5599..5797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             5815..5995
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6042..6167
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6664..6897
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8420..8464
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8762..8879
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
      541 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
      601 caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc atgtataaca
      661 tgatggagac ggagctgaag ccgccgggcc cgcagcaagc ttcggggggc ggcggcggag
      721 gaggcaacgc cacggcggcg gcgaccggcg gcaaccagaa gaacagcccg gaccgcgtca
      781 agaggcccat gaacgccttc atggtatggt cccgggggca gcggcgtaag atggcccagg
      841 agaaccccaa gatgcacaac tcggagatca gcaagcgcct gggcgcggag tggaaacttt
      901 tgtccgagac cgagaagcgg ccgttcatcg acgaggccaa gcggctgcgc gctctgcaca
      961 tgaaggagca cccggattat aaataccggc cgcggcggaa aaccaagacg ctcatgaaga
     1021 aggataagta cacgcttccc ggaggcttgc tggcccccgg cgggaacagc atggcgagcg
     1081 gggttggggt gggcgccggc ctgggtggcg ggctgaacca gcgcatggac agctacgcgc
     1141 acatgaacgg ctggagcaac ggcagctaca gcatgatgca ggagcagctg ggctacccgc
     1201 agcacccggg cctcaacgct cacggcgcgg cacagatgca accgatgcac cgctacgacg
     1261 tcagcgccct gcagtacaac tccatgacca gctcgcagac ctacatgaac ggctcgccca
     1321 cctacagcat gtcctactcg cagcagggca cccccggtat ggcgctgggc tccatgggct
     1381 ctgtggtcaa gtccgaggcc agctccagcc cccccgtggt tacctcttcc tcccactcca
     1441 gggcgccctg ccaggccggg gacctccggg acatgatcag catgtacctc cccggcgccg
     1501 aggtgccgga gcccgctgcg cccagtagac tgcacatggc ccagcactac cagagcggcc
     1561 cggtgcccgg cacggccaaa tacggcacac tgcccctgtc gcacatgtgc tcgagatatc
     1621 tagacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc
     1681 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     1741 cgcgtaccgg ttagtaatga caattcgtcg agggacctaa taacttcgta tagcatacat
     1801 tatacgaagt tatacatgtt taagggttcc ggttccacta ggtacaattc gatatcaagc
     1861 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     1921 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     1981 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2041 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2101 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2161 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2221 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2281 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2341 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2401 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     2461 gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca
     2521 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     2581 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     2641 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     2701 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     2761 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     2821 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     2881 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     2941 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     3001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa
     3181 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3241 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3301 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3361 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3421 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3481 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3541 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3601 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3661 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3721 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3781 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3841 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3901 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3961 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4021 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4081 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4141 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4201 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4261 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4321 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4381 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4441 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4501 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4561 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4621 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4681 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4741 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4801 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4861 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4921 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4981 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     5041 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     5101 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     5161 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     5221 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     5281 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     5341 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     5401 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     5461 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     5521 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     5581 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     5641 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     5701 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     5761 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     5821 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5881 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5941 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     6001 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     6061 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     6121 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     6181 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     6241 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     6301 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     6361 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     6421 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     6481 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     6541 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     6601 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     6661 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     6721 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     6781 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     6841 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     6901 gatttggggt tgctctggaa aactcatttg caccactgct gtgccctgat gaatccccta
     6961 atgattttta tcaaaatcat taagttaagg tagatacaca tcttgtcata tgatcaaatg
     7021 gtttcgccaa aaatcaataa tcagacaaca aaatgtgcga actcgatatt ttacacgact
     7081 ctctttacca attctgcccc gaattacact taaaacgact caacagctta acgttggctt
     7141 gccacgcctt acttgactgt aaaactctca ctcttaccga acttggccgt aacctgccaa
     7201 ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg attgttaggt aatcgtcacc
     7261 tccacaaaga gcgactcgct gtataccgtt ggcatgctag ctttatctgt tcgggcaata
     7321 cgatgcccat tgtacttgtt gactggtctg atatccgtga gcaaaaacgg cttatggtat
     7381 tgcgagcttc agtcgcacta cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc
     7441 tttcagagca atgttcaaag aaagctcatg accaatttct agccgacctt gcgagcattc
     7501 taccgagtaa caccacaccg ctcattgtca gtgatgctgg ctttaaagtg ccatggtata
     7561 aatccgttga gaagctgggt tggtactggt taagtcgagt aagaggaaaa gtacaatatg
     7621 cagacctagg agcggaaaac tggaaaccta tcagcaactt acatgatatg tcatctagtc
     7681 actcaaagac tttaggctat aagaggctga ctaaaagcaa tccaatctca tgccaaattc
     7741 tattgtataa atctcgctct aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc
     7801 accacccgtc acctaaaatc tactcagcgt cggcaaagga gccatggatt ctagcaacta
     7861 acttacctgt tgaaattcga acacccaaac aacttgttaa tatctattcg aagcgaatgc
     7921 agattgaaga aaccttccga gacttgaaaa gtcctgccta cggactaggc ctacgccata
     7981 gccgaacgag cagctcagag cgttttgata tcatgctgct aatcgccctg atgcttcaac
     8041 taacatgttg gcttgcgggc gttcatgctc agaaacaagg ttgggacaag cacttccagg
     8101 ctaacacagt cagaaatcga aacgtactct caacagttcg cttaggcatg gaagttttgc
     8161 ggcattctgg ctacacaata acaagggaag acttactcgt ggctgcaacc ctactagctc
     8221 aaaatttatt cacacatggt tacgctttgg ggaaattatg aggggatctc tcagtgctgt
     8281 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8341 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8401 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8461 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8521 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8581 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     8641 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     8701 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     8761 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     8821 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     8881 gggtttatta cagggacagc agagatccag tttggttagt accgggcccg ctctagcagt
     8941 tccg
//
