LOCUS       sequence-385304-       16687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3
            promtoer in M. oryzae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16687)
  AUTHORS   Zhang YM, Zheng L, Xie K
  TITLE     CRISPR/dCas9-Mediated Gene Silencing in Two Plant Fungal Pathogens.
  JOURNAL   mSphere. 2023 Feb 21;8(1):e0059422. doi: 10.1128/msphere.00594-22. 
            Epub 2023 Jan 19.
  PUBMED    36655998
REFERENCE   2  (bases 1 to 16687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2023-02-21"; volume: "8(1):e0059422. doi"; pages: " 
            10.1128/msphere.00594-22. Epub 2023 Jan 19"
COMMENT     Sequence Label: pIF3
FEATURES             Location/Qualifiers
     source          1..16687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      163..730
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     protein_bind    803..824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(839..844,845..862,863..869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    877..893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     882..904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     901..917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     901..917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1791..2918
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             2925..2978
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2979..4004
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2979..4004
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      4174..4741
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     complement(4810..4829)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5068..5085)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5068..5084)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5077..5099)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5287..5311
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6612..7241
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7670..8743
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8809..9003
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9239..9261)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9347..9487
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9402..9421
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9502..9519)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9673..10261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9753..9772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10348..11142)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11567..11591
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    12267..12291
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             12315..12341
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12345..12365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(12375..12398,12399..12401,12402..14957,14958..14960,
                     14961..16475)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(12375..12398,12399..12401,12402..14888,14889..14891,
                     14892..16475)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             16479..16499
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             16524..16646
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     protein_bind    complement(join(16677..16687,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtacttaa cgttactgaa atcatcaaac agcttgacga atctggatat
       61 aagatcgttg gtgtcgatgt cagctccgga gttgagacaa atggtgttca ggatctcgat
      121 aagatacgtt catttgtcca agcagcaaag agtgccttct agtgatttaa tagctccatg
      181 tcaacaagaa taaaacgcgt ttcgggttta cctcttccag atacagctca tctgcaatgc
      241 attaatgcat tggacctcgc aaccctagta cgcccttcag gctccggcga agcagaagaa
      301 tagcttagca gagtctattt tcattttcgg gagacgagat caagcagatc aacggtcgtc
      361 aatggaccta cgagactgag gaatccgctc ttggctccac gcgactatat atttgtctct
      421 aattgtactt tgacatgctc ctcttcttta ctctgatagc ttgactatga aaattccgtc
      481 accagcccct gggttcgcaa agataattgc actgtttctt ccttgaactc tcaagcctac
      541 aggacacaca ttcatcgtag gtataaacct cgaaaatcat tcctactaag atgggtatac
      601 aatagtaacc atggttgcct agtgaatgct ccgtaacacc caatacgccg gccgaaactt
      661 ttttacaact ctcctatgag tcgtttaccc agaatgcaca ggtacacttg tttagaggta
      721 atccttcttt ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      781 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      841 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      901 caggaaacag ctatgacatg attacgaatt cgagctcggt acccggggat cctctagagt
      961 cgacctgcag gcatgcacac tacgcgaatc atgtctctga gagtattcta gctgattggc
     1021 gaatgtctaa gcaaatggat gcagagaacc actcgatctt cgtgtctcaa tcataggcgg
     1081 aattagattc ccatataaag agaatgctgc cccacaatgg gcccgcccaa cggcaaagga
     1141 tgtgggtgtg tattagtcag ctgcaaactt ttcttgcaca ggccagacag ttaccccact
     1201 tgtagagagt ataagaatac ctgctcgccc tctccgaatc gtttttgtgt ttctctttct
     1261 accatgtcga tcttacttca aagtgagtct cccatacaca gcctcttcac ctctccagcc
     1321 tttccaagca acaccatcac taaggagacc gaggtctcgg ttttgaattg ggtactcaaa
     1381 ttggttcccg cttgacgaca ttccgaaacc cccaatttcg cggcttcgaa tcgtggctac
     1441 ccttggccga ggttgcgatt tctctgccgt atctgacaat gatccaaccg gccggatgtg
     1501 tgggagggcg caaagggtgt aattgggtct aggcgtgagc tgcatgtccg atggcagaaa
     1561 aagaactact aaaactctgt gacgtcctca gggctggtag ctatttagct tgcccctccc
     1621 tcaccccacc tagctcaaac ccaacaaaac acagcaccga ccacaaaaat ctgcctctta
     1681 ccacctgctc atcacctttt ctcacatata aagcagtgag ctcagcccga tttccattcc
     1741 tcaattcaag tctattaact ctctcaaaga ggaacccaat cttcagatcc atggcttcgt
     1801 acccctgcca tcaacacgcg tctgcgttcg accaggctgc gcgttctcgc ggccatagca
     1861 accgacgtac ggcgttgcgc cctcgccggc agcaagaagc cacggaagtc cgcctggagc
     1921 agaaaatgcc cacgctactg cgggtttata tagacggtcc tcacgggatg gggaaaacca
     1981 ccaccacgca actgctggtg gccctgggtt cgcgcgacga catcgtctac gtacccgagc
     2041 cgatgactta ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc tacaccacac
     2101 aacaccgcct cgaccagggt gagatttcgg ccggggacgc ggcggtggta atgacaagcg
     2161 cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct cctcatatcg
     2221 ggggggaggc tggcagctca catgccccgc ccccggccct caccctcatc ttcgaccgcc
     2281 atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc agcatgaccc
     2341 cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc acaaacatcg
     2401 tgttgggggc ccttccagag gacagacaca tcgaccgcct ggccaaacgc cagcgccccg
     2461 gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttacggg ctgcttgcca
     2521 atacggtgcg gtatctccag ggcggcgggt cgtggcggga ggattgggga cagctttcgg
     2581 ggacggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca cgaccccata
     2641 tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc aacggcgacc
     2701 tgtacaacgt gtttgcctgg gccttggacg tcttggccaa acgcctccgt cccatgcacg
     2761 tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg ctgcaactta
     2821 cctccgggat ggtccagacc cacgtcacca cccccggctc cataccgacg atctgcgacc
     2881 tggcgcgcac gtttgcccgc gagatggggg aggctaaccg tgccgagggt cgtggttcgc
     2941 tcctcacctg tggtgacgtc gaggagaacc ctggtcctat gaaaaagcct gaactcaccg
     3001 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     3061 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     3121 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     3181 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     3241 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     3301 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     3361 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     3421 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     3481 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     3541 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     3601 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     3661 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     3721 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     3781 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     3841 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgta gggcgtacac
     3901 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     3961 gtggaaaccg acgccccagc actcgtccga gggcaaagga atagagtaga tgccgaccgg
     4021 gatccactta acgttactga aatcatcaaa cagcttgacg aatctggata taagatcgtt
     4081 ggtgtcgatg tcagctccgg agttgagaca aatggtgttc aggatctcga taagatacgt
     4141 tcatttgtcc aagcagcaaa gagtgccttc tagtgattta atagctccat gtcaacaaga
     4201 ataaaacgcg tttcgggttt acctcttcca gatacagctc atctgcaatg cattaatgca
     4261 ttggacctcg caaccctagt acgcccttca ggctccggcg aagcagaaga atagcttagc
     4321 agagtctatt ttcattttcg ggagacgaga tcaagcagat caacggtcgt caagagagtt
     4381 acgagactga ggaatccgct cttggctcca cgcgactata tatttgtctc taattgtact
     4441 ttgacatgct cctcttcttt actctgatag cttgactatg aaaattccgt caccagcccc
     4501 tgggttcgca aagataattg cactgtttct tccttgaact ctcaagccta caggacacac
     4561 attcatcgta ggtataaacc tcgaaaatca ttcctactaa gatgggtata caatagtaac
     4621 catggttgcc tagtgaatgc tccgtaacac ccaatacgcc ggccgaaact tttttacaac
     4681 tctcctatga gtcgtttacc cagaatgcac aggtacactt gtttagaggt aatccttctt
     4741 tctagaggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     4801 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcac cacttcgggc
     4861 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     4921 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     4981 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat ctagaggatc
     5041 ccccgactag tgctgaggca ttatggcact ggccgtcgtt ttacaacgtc gtgactggga
     5101 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5161 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5221 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc
     5281 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     5341 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     5401 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
     5461 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     5521 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     5581 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     5641 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     5701 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     5761 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     5821 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     5881 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     5941 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     6001 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     6061 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     6121 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     6181 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     6241 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     6301 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     6361 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     6421 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     6481 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     6541 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     6601 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     6661 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     6721 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     6781 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     6841 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     6901 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     6961 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     7021 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     7081 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     7141 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     7201 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     7261 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     7321 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     7381 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     7441 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     7501 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     7561 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     7621 cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     7681 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     7741 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     7801 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     7861 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     7921 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     7981 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     8041 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     8101 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     8161 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     8221 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     8281 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     8341 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     8401 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     8461 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     8521 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     8581 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     8641 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     8701 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     8761 agggcaaatt gccctagcag gggaaaaagg tcgaaaagca ctctttcctg tggatagcac
     8821 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     8881 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     8941 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     9001 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     9061 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     9121 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     9181 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     9241 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     9301 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     9361 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     9421 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9481 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9541 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9601 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9841 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10021 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10081 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10141 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10201 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10321 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
    10381 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    10441 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    10501 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    10561 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    10621 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    10681 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    10741 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    10801 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    10861 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    10921 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    10981 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    11041 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    11101 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    11161 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    11221 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    11281 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    11341 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    11401 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    11461 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    11521 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    11581 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    11641 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
    11701 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
    11761 ttatatgctc aacacatgag cggaattcat aaatgtaggt attacctgta cattttattt
    11821 attcgagaaa aacaaacaaa ccaaaacgat gcagcgatca ctaccggcgt catccggaat
    11881 tgaaacggcg cgcgttgagg ggggccttct tgttcggaca cacacggggc cgccaaagtg
    11941 gggcccatac ggctgcccat cccttgctct ccaataccct ggtcatgtga ctcgaaattg
    12001 gagactcaca aagatgcttt gaaaccccgc cgggaacttt ttctcacaga cacaatcagt
    12061 cgaccttatc atcgcaaaat cgacaaacct caaaaagaac tcgaagagtc agtctccctt
    12121 gcggttaaac tgattgatat ctcgtgttct ctaaaccttc aataagtgag ttactgcgca
    12181 caaagccgac tatactgagc accaacgcga attgctcacg ttgtcgccta acagatcttg
    12241 gctttcgtag gaacccaatc ttcaaaacaa gtttgtacaa aaaagcaggc tccgaattca
    12301 ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag aaaaagcgca
    12361 aggtcgaagc gtccgacaag aagtacagca tcggcctggc catcggcacc aactctgtgg
    12421 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
    12481 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
    12541 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
    12601 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
    12661 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
    12721 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
    12781 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
    12841 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
    12901 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
    12961 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
    13021 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
    13081 tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
    13141 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
    13201 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
    13261 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
    13321 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
    13381 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
    13441 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
    13501 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
    13561 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
    13621 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
    13681 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
    13741 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
    13801 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
    13861 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
    13921 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
    13981 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
    14041 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
    14101 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
    14161 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
    14221 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
    14281 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
    14341 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
    14401 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
    14461 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
    14521 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
    14581 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
    14641 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
    14701 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
    14761 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
    14821 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
    14881 atgtggacgc catcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
    14941 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
    15001 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
    15061 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
    15121 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
    15181 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
    15241 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
    15301 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
    15361 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
    15421 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
    15481 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
    15541 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
    15601 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
    15661 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
    15721 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
    15781 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
    15841 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
    15901 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
    15961 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
    16021 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
    16081 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
    16141 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
    16201 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
    16261 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
    16321 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
    16381 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
    16441 agacacggat cgacctgtct cagctgggag gcgacagccc caagaagaag agaaaggtgg
    16501 aggccagcgg gccggccgga tccatggaac gtgtgagaat gattaatgtg caaaggctgt
    16561 tagaagccgc agagttttta gaaagaagag aaagagaatg cgaacacggg tatgccagtt
    16621 ctttccctag catgccctct cccagaggct aaatcgattg attaattaag aattcgaccc
    16681 agctttc
//
