LOCUS       sequence-385325-        8935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant 
            ""Patchless"" (K184Rfs*44, ""Patchless"" variant) Fusion Protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Mensah MA, Niskanen H, Magalhaes AP, Basu S, Kircher M, Sczakiel HL,
            Reiter AMV, Elsner J, Meinecke P, Biskup S, Chung BHY, Dombrowsky G,
            Eckmann-Scholz C, Hitz MP, Hoischen A, Holterhus PM, Hulsemann W, 
            Kahrizi K, Kalscheuer VM, Kan A, Krumbiegel M, Kurth I, Leubner J, 
            Longardt AC, Moritz JD, Najmabadi H, Skipalova K, Snijders Blok L, 
            Tzschach A, Wiedersberg E, Zenker M, Garcia-Cabau C, Buschow R, 
            Salvatella X, Kraushar ML, Mundlos S, Caliebe A, Spielmann M, Horn 
            D, Hnisz D
  TITLE     Aberrant phase separation and nucleolar dysfunction in rare genetic 
            diseases.
  JOURNAL   Nature. 2023 Feb;614(7948):564-571. doi: 10.1038/s41586-022-05682-1.
            Epub 2023 Feb 8.
  PUBMED    36755093
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05682-1"; journalName: "Nature"; date: 
            "2023-02"; volume: "614"; issue: "7948"; pages: "564-571"
COMMENT     Sequence Label: pPB-TetON-mEGFP-HMGB1-MUT-Patchless
FEATURES             Location/Qualifiers
     source          1..8935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   147..209
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        658..1036
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    666..684
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    702..720
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    738..756
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    774..792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    810..828
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    846..864
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    882..900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     945..969
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1049..1058
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1055..1057,1058..1060,1061..1768)
                     /codon_start=1
                     /product="enhanced GFP (EGFP) derivative that exhibits 
                     robust folding and enhanced photostability (Zhong et al., 
                     2019)"
                     /label=SiriusGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1100..1121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1708..1729
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2499..2518)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2543..2591
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2822..3033
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3046..3314
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3348..4091
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             4092..4145
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4146..4745
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4146..4165)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4642..4662
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4778..4899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4815..4834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4869..4888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5662..5696
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6187..6203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6187..6203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6200..6222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6211..6227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6235..6241,6242..6259,6260..6265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6280..6301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6418..6435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6589..7177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6669..6688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7348..8139,8140..8208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7971..7990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8209..8313)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8339..8794)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8476..8497)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8688..8707
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(8921..8935,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(8935,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg
       61 ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca
      121 cgctgcagaa cacgcagcta gattaaccct agaaagataa tcatattgtg acgtacgtta
      181 aagataatca tgtgtaaaat tgacgcatgt gttttatcgg tctgtatatc gaggtttatt
      241 tattaatttg aatagatatt aagttttatt atatttacac ttacatacta ataataaatt
      301 caacaaacaa tttatttatg tttatttatt tattaaaaaa aacaaaaact caaaatttct
      361 tctataaagt aacaaaactt ttatgaggga cagccccccc ccaaagcccc cagggatgta
      421 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
      481 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
      541 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
      601 ggggatacgg ggaaaaggcc tccacggcca ctagtctttc gtcttcaaga attcctcgag
      661 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
      721 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
      781 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
      841 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
      901 acgtataagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac
      961 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
     1021 ttcctaccct cgtaaaggtc tagagctagc caccatggtg agcaagggcg aggagctgtt
     1081 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1141 cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg
     1201 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1261 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1321 gcccgaaggc tacgtccagg agcgcaccat ctccttcaag gacgacggca cctacaagac
     1381 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1441 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca
     1501 caacgtctat atcacggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg
     1561 ccacaacgtc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1621 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
     1681 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1741 gatcactctc ggcatggacg agctgtacac ggcgatgggc aaaggagatc ctaagaagcc
     1801 gagaggcaaa atgtcatcat atgcattttt tgtgcaaact tgtcgggagg agcataagaa
     1861 gaagcaccca gatgcttcag tcaacttctc agagttttct aagaagtgct cagagaggtg
     1921 gaagaccatg tctgctaaag agaaaggaaa atttgaagat atggcaaaag cggacaaggc
     1981 ccgttatgaa agagaaatga aaacctatat ccctcccaaa ggggagacaa aaaagaagtt
     2041 caaggatccc aatgcaccca agaggcctcc ttcggccttc ttcctcttct gctctgagta
     2101 tcgcccaaaa atcaaaggag aacatcctgg cctgtccatt ggtgatgttg cgaagaaact
     2161 gggagagatg tggaataaca ctgctgcaga tgacaagcag ccttatgaaa agaaggctgc
     2221 gaagctgaag gaaaaatatg aaaaggatat tgctgcatat cgagctaaag gaaagcctga
     2281 tgcagcaaaa aagggagttg tcaaggctga aaaaagcaag aaaaggaaga ggaggaagat
     2341 gaggaagatg aagaggatga ggaggaggag gaagatgaag aagatgaaga tgaagaagaa
     2401 gtaatgtaca agaccggttg accggttagt aatgagttta aacgggggag gaagaccggt
     2461 tgaccggtta gtaatgagtt taaacggggg aggctaactg aaacacggaa ggagacaata
     2521 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     2581 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     2641 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     2701 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga
     2761 tctgcgaaat cggatccagc tgaatctaag tggatctgcg atcgctccgg tgcccgtcag
     2821 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     2881 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     2941 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     3001 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     3061 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     3121 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     3181 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     3241 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     3301 cgccgttaca gatctaagct gtgaccggcg cctactctag agccgccatg tctagactgg
     3361 acaagagcaa agtcataaac tctgctctgg aattactcaa tggagtcggt atcgaaggcc
     3421 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     3481 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     3541 cccactcctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     3601 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     3661 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     3721 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     3781 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     3841 ctatgccccc acttctgaaa caagcaattg agctgttcga ccggcaggga gccgaacctg
     3901 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     3961 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     4021 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     4081 tgctccccgg ggagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     4141 gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     4201 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     4261 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4321 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4381 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4441 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4501 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4561 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4621 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4681 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4741 cctgaaatca acctctggat tacaaaattt gtgggtaaac ttgtttattg cagcttataa
     4801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa
     4921 atgatgtcaa ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc
     4981 ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc
     5041 aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag
     5101 ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt
     5161 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
     5221 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
     5281 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
     5341 gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca
     5401 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
     5461 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
     5521 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
     5581 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
     5641 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
     5701 tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct
     5761 ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
     5821 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
     5881 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
     5941 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
     6001 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
     6061 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
     6121 ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta
     6181 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6241 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6301 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6361 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6421 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6481 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6541 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6601 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6661 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6721 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6781 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6841 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6901 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6961 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7021 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7081 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7141 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7201 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7261 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7321 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7381 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7441 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7501 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7561 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7621 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7681 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7741 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7801 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7861 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7921 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7981 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8041 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8101 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8161 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8221 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8281 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     8341 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     8401 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8461 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8521 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8581 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8641 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8701 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8761 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     8821 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     8881 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgtt
//
