LOCUS       sequence-385353-        2821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Gateway with Eps8L2_529-715.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, 
            Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
  TITLE     Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear 
            positioning during cell migration.
  JOURNAL   Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 
            10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.
  PUBMED    33567288
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.01.007"; journalName: "Curr Biol"; date: 
            "2021-04-12- 12"; volume: "31"; issue: "7"; pages: "1521-1530.e8"
COMMENT     Sequence Label: pDONR201-Eps8L2_529-715
FEATURES             Location/Qualifiers
     source          1..2821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(449..548)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(660..679)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             671..1480
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(738..757)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1623..2211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2112..2131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2346..2373)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2465..2551)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    2604..2703
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gggccccgcc agcccgaccc acaagctacc cccaagcttc ccgggcaata aggacgaact
       61 catgcagcac atggacgagg tcaacgacga gctcatccgc aagatatcga atataagggc
      121 gcagccacag aggcacttcc gcgtggagcg cagccagccc gtgagccagc cgctcaccta
      181 cgagtcgggt ccggacgagg tccgcgcctg gctggaagcc aaggccttca gcccgcggat
      241 cgtggagaac ctgggcatcc tgaccgggcc gcagctcttc tccctcaaca aggaggagct
      301 gaagaaagtg tgcggcgagg agggcgtccg cgtgtacagc cagctcacca tgcagaaggc
      361 cttcctggag aagcagcaaa gtgggtcgga gctggaagaa ctcatgaaca agtttcattc
      421 catgaatcag aggagggggg aggacagcac ccagctttct tgtacaaagt tggcattata
      481 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      541 attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat gttacattgc
      601 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
      661 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
      721 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
      781 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
      841 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
      901 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
      961 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     1021 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     1081 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     1141 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     1201 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     1261 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     1321 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     1381 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     1441 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     1501 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     1561 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1621 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1681 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1741 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1801 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1861 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1921 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1981 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2041 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2101 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2161 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2221 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2281 gcgttatccc ctgattctgt ggataaccgt attaccgcta gccaggaaga gtttgtagaa
     2341 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     2401 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     2461 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     2521 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     2581 acgctagcat ggatctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt
     2641 cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagcag
     2701 gctctatgtg gaagctgcgc agccgcagcg gccaggcggg gtacgtgccc tgcaacatcc
     2761 taggcgaggc gcgaccggag gacgccggcg ccccgttcga gcaggccggt cagaagtact
     2821 g
//
