LOCUS       sequence-385367-        3624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_11 [pWKS1 ORI]
FEATURES             Location/Qualifiers
     source          1..3624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(413..507)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(528..1187)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1102..1121
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1188..1290)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1377..1399
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1391..1408
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1392..1408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3128..3144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3128..3144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3141..3163)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(join(3316..3624,1..280))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3396..3415)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
       61 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      121 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      301 tacggggtct gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt
      361 agggggcttc aaatcgtccc cccatacgat ataagttgtt actagtgctt ggattctcac
      421 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
      481 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
      541 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
      601 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
      661 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
      721 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
      781 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
      841 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
      901 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
      961 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1021 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     1081 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     1141 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     1201 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     1261 tgaaagttgg aacctcttac gtgccgatca acgtctcatt gatacctgaa acaaaaccca
     1321 tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca
     1381 gtcacgacgt tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga
     1441 agtgccattc cgcctgacct tagcgtgcga acagcacgct attgttgtat cattactcgc
     1501 atccattctc aggctgtctc gtctcgtctc cgaggtctcc ggaggccgat ccgccgccag
     1561 ttcaggccgt ccggccccgg attctgacca taatttcatc gaaaaaaggg gcgcagccct
     1621 tcttgttcta atagttctat aagttcaggc gaaaatcgtg cagcaattac aaaaggttgc
     1681 gcgtctataa gtggggaatc cagccgcaaa agtggggaat ccagccgcaa aagtggggaa
     1741 tccagccgaa atcgcggatt gacgagtggg atttcccgcc caataaatcc accatgggaa
     1801 agacactcga cgttgcccgc gaccgggcct ttgaccagac cgcgaccgtg ctgcccgccg
     1861 aaatggcgcg gggggtctat atgcgcaacg cccccagcct cgcggccctg aagctgatgc
     1921 atctgatgat cgccacggcg ggcgggcgca tggccgatga cgtgcgccac gaaatgcggc
     1981 tggccgacat ccgcaagatc gacggtatgg ataaccacac ccgggccagc ctgaccccgc
     2041 tctttgcgga actggcggcg gcggtgctga cccacgacga cccggaaaag cgggtcgtga
     2101 ccatcggtgg cctgctggac gaagcccgga tcgattaccg gcacgaggtc agcggcgatc
     2161 ttctggtgtc gtggaccttc cgcagcatgt tccgccgcat ggcggcggaa tcgaaccatt
     2221 gggcgattct cgaccggcag accgtgttcc acctcggcag caagtattcc gtgctgttgt
     2281 tccagcacat cgccagcttc aaggaatacg accacattac cggcaagacc tttaccgtgc
     2341 cggagttgcg ggctgtgttt ggtatccccg agggcaaaat caagcgtttc gcagacctca
     2401 acagagacgt gctgacgccc gccattgccg aaatcaacca gctttcccgc ctgactctga
     2461 ccgccacgcc gaacaagatc gggcgcaccg tggccagcgt gacgattgct tgggaagaaa
     2521 agcccctcga aggcaagcgc tcgaccaagg ccgaactgga ccgcccgaag gtgggccgga
     2581 aggcccggcg cgacggcacc gccgagacgg tggcacgggc cttcccggca tcgggcggga
     2641 tcgagttcga ccagcattgg cgcgacctca agcgggcggc gggctgcaac atggacaaca
     2701 ccatgatcgc cgacaaattc cgggcatggt gcgccgggaa gggcctcgct ctcgatgccc
     2761 ggaacatcga acaggcgttc agcagcttct gcaccaaggt gggccgggtc taagacccgc
     2821 cgcgccggtc gctcgatacc tgtggtctgg ctccctctgc ggctaccgtc agcgcctcgc
     2881 ctgcatcgcc gccctttccg atcctcatcc cgccccagcc ttatgggggg atgaggatcg
     2941 ggccgggact gaaacccgaa gggtaatgaa tgtgtctttc cctgccgctc gagaccaccc
     3001 caggatacat agattaccac aactccgagc ccttccacct agcgtgctaa ccacgcacgc
     3061 tattgttgta taggctaggt ggaggctcag tgatgataag tctgcgatgg tggatgcatg
     3121 tgtcatggtc atagctgttt cctgtgtgaa attgttatcc gctcagaggg cacaatccta
     3181 ttccgcgcta tccgacaatc tccaagacat taggtggagt tcagttcggc gtatggcata
     3241 tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3301 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3361 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3421 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3481 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3541 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3601 cgccttatcc ggtaactatc gtct
//
