LOCUS       sequence-38547-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila 
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 May;36(8):2547-60. doi: 10.1093/nar/gkn048. 
            Epub 2008 Mar 10.
  PUBMED    18332042
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn048"; journalName: "Nucleic Acids Res"; date: 
            "2008-05"; volume: "36"; issue: "8"; pages: "2547-60"
COMMENT     Sequence Label: pB1H2w2-zif268
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(479..502)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(512..781)
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
ORIGIN
        1 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
       61 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      121 tattacgcca ggatcttcag cggagcggga cttgtcggcc tttttctagt ctctagacgt
      181 ccttctgtct taaatggatt ttggtatgcc tcttgcgttc atcactcctg gcaaacttcc
      241 tcccacaaat gtcacaggca aaaggcttct cgcctgtgtg ggtgcggatg tgggtggtaa
      301 ggtggtcact acgactgaag ttacgcatgc agattcgaca ctggaagggc ttctgaccgg
      361 tatggatgcg gatatggcgg gtaagctcat ccgagcgaga aaagcggcga tcgcaggact
      421 cgacagggca agcatatggg cgggtaccat gggggggtgt cttggaaccg gtccggaact
      481 tgtcgtcgtc atccttgtag tccgcggccg cacgaccttc agcaatagcg gtaacggctt
      541 gtaattcagc ggcttcctgc tcttgctgtt cctggcgttc gcgaacgtcg aggatctggt
      601 tgttgatcag accttcttcg atttcgcgca gcgcgattac agtggtttta tcgttttctt
      661 ccggtacgag cgggtccttt ccgcctacct gcatctgacg agcgcgacgc gcggcgacca
      721 gtaccaggtc aaaacggtta ccaattttct ctacagcgtc ctgaacagtt acgcgtgcca
      781 tgggtatatc tccttcttgg agccgagact tacaattgtg ttttttcaaa attcttgcag
      841 gtttcgatgg cttcttgtaa taagctgcgt tgctcctctg
//
