LOCUS       sequence-38550-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Pasque V, Gillich A, Garrett N, Gurdon JB
  TITLE     Histone variant macroH2A confers resistance to nuclear 
            reprogramming.
  JOURNAL   EMBO J. 2011 May 6;30(12):2373-87. doi: 10.1038/emboj.2011.144.
  PUBMED    21552206
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-05-6- 6"; 
            volume: "30"; issue: "12"; pages: "2373-87"
COMMENT     Sequence Label: pSUPER retro puro GFP shRNA
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..61
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     46..62
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(131..345)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(191..210)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        356..855
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(391..414)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     788..807
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ccagccctca ctccttctct aggcgccgga attagatcga tctctcgagg tcgacggtat
       61 cgataagctt aaaaaggcac aagctggagt acaatctctt gaattgtact ccagcttgtg
      121 ccggggatct gtggtctcat acagaactta taagattccc aaatccaaag acatttcacg
      181 tttatggtga tttcccagaa cacatagcga catgcaaata ttgcagggcg ccactcccct
      241 gtccctcaca gccatcttcc tgccagggcg cacgcgcgct gggtgttccc gcctagtgac
      301 actgggcccg cgattccttg gagcgggttg atgacgtcag cgttcgaatt ctaccgggta
      361 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
      421 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
      481 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
      541 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
      601 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
      661 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
      721 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
      781 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
      841 tctccgggcc tttcgacctg cagcccaagc tagcttacca tgacc
//
