LOCUS       sequence-38551-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Pasque V, Gillich A, Garrett N, Gurdon JB
  TITLE     Histone variant macroH2A confers resistance to nuclear 
            reprogramming.
  JOURNAL   EMBO J. 2011 May 6;30(12):2373-87. doi: 10.1038/emboj.2011.144.
  PUBMED    21552206
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-05-6- 6"; 
            volume: "30"; issue: "12"; pages: "2373-87"
COMMENT     Sequence Label: pSUPER retro puro macroH2A2 shRNA
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..57
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     42..58
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(154..368)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(214..233)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        379..878
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(414..437)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     811..830
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatcgatctc tcgaggtcga cggtatcgat
       61 aagcttcttg tcctctgctt tttaagctta aaaagcagag gacaagaagc ttaaatctct
      121 tgaatttaag cttcttgtcc tctgcgggga tctgtggtct catacagaac ttataagatt
      181 cccaaatcca aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa
      241 atattgcagg gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc
      301 gctgggtgtt cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt
      361 cagcgttcga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
      421 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
      481 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
      541 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
      601 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
      661 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
      721 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc angggcgggg
      781 cgggcgcccg aaggtcctcc ggangcccgg cattctgcac gcttcaaaag cgcacgtctg
      841 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcg
//
