LOCUS       sequence-38552-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Pasque V, Gillich A, Garrett N, Gurdon JB
  TITLE     Histone variant macroH2A confers resistance to nuclear 
            reprogramming.
  JOURNAL   EMBO J. 2011 May 6;30(12):2373-87. doi: 10.1038/emboj.2011.144.
  PUBMED    21552206
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-05-6- 6"; 
            volume: "30"; issue: "12"; pages: "2373-87"
COMMENT     Sequence Label: pSUPER retro puro Scr shRNA
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..58
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     43..59
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(128..342)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(188..207)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(388..411)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     785..804
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatcgatct ctcgaggtcg acggtatcga
       61 taagcttaaa aagcgaaaga tgataagcta atctcttgaa ttagcttatc atctttcgcg
      121 gggatctgtg gtctcataca gaacttataa gattcccaaa tccaaagaca tttcacgttt
      181 atggtgattt cccagaacac atagcgacat gcaaatattg cagggcgcca ctcccctgtc
      241 cctcacagcc atcttcctgc cagggcgcac gcgcgctggg tgttcccgcc tagtgacact
      301 gggcccgcga ttccttggag cgggttgatg acgtcagcgt tcgaattcta ccgggtaggg
      361 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      421 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      481 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      541 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      601 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
      661 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
      721 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
      781 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
//
