LOCUS       sequence-385552-        4399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Gateway with Eps8L2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, 
            Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
  TITLE     Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear 
            positioning during cell migration.
  JOURNAL   Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 
            10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.
  PUBMED    33567288
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.01.007"; journalName: "Curr Biol"; date: 
            "2021-04-12- 12"; volume: "31"; issue: "7"; pages: "1521-1530.e8"
COMMENT     Sequence Label: pDONR201-Eps8L2
FEATURES             Location/Qualifiers
     source          1..4399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(64..150)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    203..302
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2447..2546)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2658..2677)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2669..3478
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2736..2755)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3621..4209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4110..4129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4344..4371)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
       61 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      121 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
      181 cgctagcatg gatctcgggc cccaaataat gattttattt tgactgatag tgacctgttc
      241 gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagcagg
      301 cttgagccag tccggggccg tgagctgctg cccgggtgcc accaatggca gcctgggccg
      361 gtccgacggt gtggccaaga tgagccccaa ggacctgttt gagcagagga agaagtattc
      421 caactccaac gtcatcatgc acgagacctc gcagtaccac gtccagcacc tggccacatt
      481 catcatggac aagagcgaag ccatcacgtc tgtggacgac gccatccgga agctggtgca
      541 gctgagctcc aaggagaaga tctggaccca ggagatgctg ctgcaggtga acgaccagtc
      601 gctgcggctg ctggacatcg agtcacagga ggagctggaa gacttcccgc tgcccacggt
      661 gcagcgcagc cagacggtcc tcaaccagct gcgctacccg tctgtgctgc tgctcgtgtg
      721 ccaggactcg gagcagagca agccggatgt ccacttcttc cactgcgatg aggtggaggc
      781 agagctggtg cacgaggaca tcgagagcgc gttggccgac tgccggctgg gcaagaagat
      841 gcggccgcag accctgaagg gacaccagga gaagattcgg cagcggcagt ccatcctgcc
      901 tcctccccag ggcccggcgc ccatcccctt ccagcaccgc ggcggggatt ccccggaggc
      961 caagaatcgc gtgggcccgc aggtgccact cagcgagcca ggtttccgcc gtcgggagtc
     1021 gcaggaggag ccgcgggccg tgctggctca gaagatagag aaggagacgc aaatcctcaa
     1081 ctgcgccctg gacgacatcg agtggtttgt ggcccggctg cagaaggcag ccgaggcttt
     1141 caagcagctg aaccagcgga aaaaggggaa gaagaagggc aagaaggcgc cagcagaggg
     1201 cgtcctcaca ctgcgggcac ggcccccctc tgagggcgag ttcatcgact gcttccagaa
     1261 aatcaagctg gcgattaact tgctggcaaa gctgcagaag cacatccaga accccagcgc
     1321 cgcggagctc gtgcacttcc tcttcgggcc tctggacctg atcgtcaaca cctgcagtgg
     1381 cccagacatc gcacgctccg tctcctgccc actgctctcc cgagatgccg tggacttcct
     1441 gcgcggccac ctggtcccta aggagatgtc gctgtgggag tcactgggag agagctggat
     1501 gcggccccgt tccgagtggc cgcgggagcc acaggtgccc ctctacgtgc ccaagttcca
     1561 cagcggctgg gagcctcctg tggatgtgct gcaggaggcc ccctgggagg tggaggggct
     1621 ggcgtctgcc cccatcgagg aggtgagtcc agtgtcgagg caaagtatcc ggaactccca
     1681 gaagcacagc cccacttcag agcccacccc cccgggggat gccctaccac cagtcagctc
     1741 cccacatact cacaggggct accagccaac accagccatg gccaaatatg tgaaaattct
     1801 ctatgacttc acagcccgaa atgccaacga gctatcggtg ctcaaggatg aggtcctaga
     1861 ggtgctggag gacggccggc agtggtggaa gctgcgcagc cgcagcggcc aggcggggta
     1921 cgtgccctgc aacatcctag gcgaggcgcg accggaggac gccggcgccc cgttcgagca
     1981 ggccggtcag aagtactggg gccccgccag cccgacccac aagctacccc caagcttccc
     2041 gggcaataag gacgaactca tgcagcacat ggacgaggtc aacgacgagc tcatccgcaa
     2101 gatatcgaat ataagggcgc agccacagag gcacttccgc gtggagcgca gccagcccgt
     2161 gagccagccg ctcacctacg agtcgggtcc ggacgaggtc cgcgcctggc tggaagccaa
     2221 ggccttcagc ccgcggatcg tggagaacct gggcatcctg accgggccgc agctcttctc
     2281 cctcaacaag gaggagctga agaaagtgtg cggcgaggag ggcgtccgcg tgtacagcca
     2341 gctcaccatg cagaaggcct tcctggagaa gcagcaaagt gggtcggagc tggaagaact
     2401 catgaacaag tttcattcca tgaatcagag gaggggggag gacagcaccc agctttcttg
     2461 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     2521 atcagtcaaa ataaaatcat tatttgccat ccagctgcag ctctggcccg tgtctcaaaa
     2581 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     2641 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     2701 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     2761 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     2821 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     2881 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     2941 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     3001 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     3061 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     3121 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     3181 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     3241 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     3301 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     3361 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     3421 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     3481 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     3541 aagctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3601 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3661 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3721 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3781 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3841 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3901 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3961 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4021 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4081 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4141 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4201 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4261 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc
     4321 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     4381 gatgcctggc agtttatgg
//
