LOCUS       sequence-38557-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Pasque V, Gillich A, Garrett N, Gurdon JB
  TITLE     Histone variant macroH2A confers resistance to nuclear 
            reprogramming.
  JOURNAL   EMBO J. 2011 May 6;30(12):2373-87. doi: 10.1038/emboj.2011.144.
  PUBMED    21552206
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-05-6- 6"; 
            volume: "30"; issue: "12"; pages: "2373-87"
COMMENT     Sequence Label: pCS2+ macroH2A1.2-GFP-HA
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..110)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(159..180)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     508..527
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     767..788
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tacgtatacg actcactata gttctagata gcgtagtctg gtacatcata agggtatgca
       61 tagtcaggta cgtcataagg ataagcgtaa tctggaacat cgtatgggta ctcgagcttg
      121 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      181 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      241 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      301 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
      361 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
      421 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      481 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      541 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      601 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
      661 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
      721 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      781 tcgaccagga tgggcaccac cccggtgaac agctcctc
//
