LOCUS       sequence-385578-        2146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries a fragment from a 24-fragment lacI/lacZ 
            cassette split that was designed as a Golden Gate assembly with high
            fidelity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, 
            Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
  TITLE     Comprehensive Profiling of Four Base Overhang Ligation Fidelity by 
            T4 DNA Ligase and Application to DNA Assembly.
  JOURNAL   ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 
            10.1021/acssynbio.8b00333. Epub 2018 Oct 29.
  PUBMED    30335370
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00333"; journalName: "ACS Synth Biol"; date: 
            "2018-11-16- 16"; volume: "7"; issue: "11"; pages: "2665-2674"
COMMENT     Sequence Label: pUC57-mini v2-HF24_F9
FEATURES             Location/Qualifiers
     source          1..2146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(391..979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(471..490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1150..1941,1942..2010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1773..1792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2011..2115)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatgg tctcgtccac gcgcgcgtac atcgggcaaa taatatcggt ggccgtggtg
       61 tcggctccgc cgccttcata ctgcaccggg cgggaaggat cgacagattt gatccagcga
      121 tacagcgcgt cgtgattagc gccgtggcct gattcattcc ccagcgacca gatgatcaca
      181 ctcgggtgat tacgatcgcg ctgcaccatt cgcgttacgc gttcgctcat cgccggtagc
      241 cagcgcggat catcggtcag acgattcatt ggcaccatgc cgtgggtttc aatattggct
      301 tcatccacca cacgagacca tcggaaagaa catgtgagca aaaggccagc aaaaggccag
      361 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      421 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      481 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      541 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      601 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      661 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      721 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      781 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      841 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      901 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      961 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1021 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1081 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1141 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1201 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1261 atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc cagatttatc
     1321 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1381 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1441 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1501 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1561 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1621 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1681 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1741 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1801 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1861 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1921 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1981 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2041 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2101 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
