LOCUS       sequence-385579-        9453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To establish all-in-one TetO-inducible cell line for Pdx1-Mafa 
            expression (Doxycycline induced; rtTA included; Blasticidin 
            resistant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Huang X, Gu W, Zhang J, Lan Y, Colarusso JL, Li S, Pertl C, Lu J, 
            Kim H, Zhu J, Breault DT, Sevigny J, Zhou Q
  TITLE     Stomach-derived human insulin-secreting organoids restore glucose 
            homeostasis.
  JOURNAL   Nat Cell Biol. 2023 Apr 27. doi: 10.1038/s41556-023-01130-y.
  PUBMED    37106062
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2023 Apr 27. doi: 10.1038/s41556-023-01130-y."
COMMENT     Sequence Label: pLenti-TetO-PM
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1416..1469
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2064..2093
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        2562..3072
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2991..3009
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3101..3496
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3512..3565
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3566..4183,4184..4189,4190..4225,4226..4228,4229..4264,
                     4265..4267,4268..4303,4304..4312)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4352..4372)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4770..4781)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4986..5219
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5291..5412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5328..5347)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5382..5401
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5452..5587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5587..5658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5659..5727,5728..6519)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5877..5896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6690..7278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7179..7198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7432..7449
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7566..7587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7602..7607,7608..7625,7626..7632)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7640..7656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7645..7667
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7664..7680
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7664..7680
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7699..7719
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7701..7719
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7747..7973
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7974..8154
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8201..8326
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8819..9052
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9237..9281
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgaattcgc cgccaccatg aacagtgagg agcagtacta cgcggccaca cagctctaca
      601 aggacccgtg cgcattccag aggggcccgg tgccagagtt cagcgctaac ccccctgcgt
      661 gcctgtacat gggccgccag cccccacctc cgccgccacc ccagtttaca agctcgctgg
      721 gatcactgga gcagggaagt cctccggaca tctccccata cgaagtgccc ccgctcgcct
      781 ccgacgaccc ggctggcgct cacctccacc accaccttcc agctcagctc gggctcgccc
      841 atccacctcc cggacctttc ccgtatggaa ccgagcctgg gggcctggaa gagcccaacc
      901 gcgtccagct ccctttcccg tggatgaaat ccaccaaagc tcacgcgtgg aaaggccagt
      961 gggcaggagg tgcttacaca gcggaacccg aggaaaacaa gaggacccgt actgcctaca
     1021 cccgggcgca gctgctggag ctggagaagg aattcttatt taacaaatac atctcccggc
     1081 cccgccgggt ggagctggca gtgatgttga acttgaccga gagacacatc aaaatctggt
     1141 tccaaaaccg tcgcatgaag tggaaaaaag aggaagataa gaaacgtagt agcgggaccc
     1201 cgagtggggg cggtgggggc gaagagccgg aacaagattg tgcggtgacc tcgggcgagg
     1261 agctgctggc agtgccaccg ctgccacctc ccggaggtgc cgtgccccca ggcgtcccag
     1321 ctgcagtccg ggagggccta ctgccttcgg gccttagcgt gtcgccacag ccctccagca
     1381 tcgcgccact gcgaccgcag gaaccccggg gatccgaggg cagaggaagt cttctaacat
     1441 gcggtgacgt ggaggagaat cccggcccta tcgatatggc cgcggagctg gcgatgggcg
     1501 cagagctgcc cagcagccca ctggccatcg agtacgtcaa cgacttcgac ctgatgaagt
     1561 tcgaggtgaa gaaggagccg cccgaggccg agcgcttctg ccaccgcctg ccgcccggct
     1621 cgctgtcctc gacgcccctc agcacgccct gctcctcggt gccctcttcg cccagcttct
     1681 gcgcacccag cccgggcaca ggcggcggcg cgggcggcgg gggcagcgcg gctcaggccg
     1741 ggggcgcccc ggggccgccg agtggaggcc ccggcactgt cgggggcgcc tcaggaaaag
     1801 cggtgctgga ggatctgtac tggatgagcg ggtaccagca ccacctgaac cccgaggcgc
     1861 tcaacctgac gccggaggac gcggtggagg cgctcatcgg cagcggccac cacggcgcgc
     1921 accacggcgc gcatcacccg gcggctgctg cggcctatga ggccttccgg ggtcagagct
     1981 tcgcgggcgg cggcggcgcg gacgacatgg gtgccggcca ccaccacggc gcacaccaca
     2041 ctgcccacca tcatcactct gcccaccatc accatcacca ccatcaccac cacggaggct
     2101 ctggccacca cggcggaggc gcgggtcacg gcggaggcgg cgcaggccac cacgtgcgct
     2161 tggaggagcg cttctccgac gaccagctgg tatccatgtc cgtgcgggag ctgaaccggc
     2221 agctccgcgg cttcagcaag gaggaggtca tccgactgaa acagaagcgg cgcacgctca
     2281 agaaccgcgg ctacgcgcag tcgtgccgct tcaagcgggt gcagcagcgg cacattctgg
     2341 agagcgagaa gtgccagctc cagagccagg tggagcagct gaagctggag gtggggcgtc
     2401 tggccaagga gcgggacctg tacaaggaga aatacgagaa gttggcgggc cggggcggcc
     2461 ccgggggcgc gggcggggcc ggcttccctc gggagccctc gccagcgcag gctggccccg
     2521 gggcggccaa aggcgcaccc gacttctttc tgtaaggatc cggggttggg gttgcgcctt
     2581 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     2641 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     2701 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
     2761 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
     2821 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
     2881 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
     2941 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
     3001 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     3061 ctctctcccc agcaattcac ccgcgcgcgg gaactaaacc atggccaagc ctttgtctca
     3121 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     3181 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     3241 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     3301 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     3361 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     3421 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     3481 ttatgtgtgg gagggcttcg aaggaagcgg agaaggtaga ggttctctcc tcacttgtgg
     3541 tgatgttgaa gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg
     3601 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
     3661 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     3721 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     3781 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     3841 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     3901 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     3961 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     4021 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     4081 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     4141 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
     4201 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
     4261 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa
     4321 ggatcgatcc aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4381 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4441 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4501 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4561 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4621 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4681 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4741 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4801 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4861 ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc
     4921 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4981 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5041 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5101 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5161 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5221 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5281 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5341 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5401 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     5461 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5521 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5581 ggaggccttt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5641 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5701 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5761 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5821 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5881 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5941 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6001 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6061 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6121 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6181 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6241 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6301 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6361 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6421 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6481 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6541 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6601 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6661 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6721 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6781 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6841 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6901 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6961 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7021 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7081 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7141 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7201 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7261 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7321 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7381 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7441 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7501 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7561 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7621 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7681 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7741 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7801 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7861 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7921 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7981 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8041 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8101 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8161 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8221 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8281 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8341 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8401 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8461 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8521 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8581 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8641 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8701 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8761 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8821 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8881 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8941 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     9001 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     9061 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     9121 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     9181 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9241 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9301 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9361 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9421 cgtttcagac ccacctccca accccgaggg gac
//
