LOCUS       sequence-385580-        1971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries a fragment from a 24-fragment lacI/lacZ 
            cassette split that was designed as a Golden Gate assembly with high
            fidelity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, 
            Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
  TITLE     Comprehensive Profiling of Four Base Overhang Ligation Fidelity by 
            T4 DNA Ligase and Application to DNA Assembly.
  JOURNAL   ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 
            10.1021/acssynbio.8b00333. Epub 2018 Oct 29.
  PUBMED    30335370
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00333"; journalName: "ACS Synth Biol"; date: 
            "2018-11-16- 16"; volume: "7"; issue: "11"; pages: "2665-2674"
COMMENT     Sequence Label: pUC57-mini v2-HF24_F1
FEATURES             Location/Qualifiers
     source          1..1971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(216..804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(296..315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(975..1766,1767..1835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1598..1617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1836..1940)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatgg tctcgggagt catttttgac accagaccaa ctggtaatgg tagcgaccgg
       61 cgctcagctg gaattccgcc gatactgacg ggctccagga gtcgtcgcca ccaatcccca
      121 tatggaaacc gtcgatacga gaccatcgga aagaacatgt gagcaaaagg ccagcaaaag
      181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      901 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      961 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1021 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1081 ttaccatctg gccccagtgc tgcaatgata ccgcgagatc cacgctcacc ggctccagat
     1141 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1201 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1261 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1321 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1381 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1441 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1501 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1561 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1621 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1681 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1741 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1801 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1861 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1921 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
