LOCUS       sequence-385581-        9762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To constitutively express Pdx1-Mafa and in cells (Blasticidin 
            resistant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Huang X, Gu W, Zhang J, Lan Y, Colarusso JL, Li S, Pertl C, Lu J, 
            Kim H, Zhu J, Breault DT, Sevigny J, Zhou Q
  TITLE     Stomach-derived human insulin-secreting organoids restore glucose 
            homeostasis.
  JOURNAL   Nat Cell Biol. 2023 Apr 27. doi: 10.1038/s41556-023-01130-y.
  PUBMED    37106062
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2023 Apr 27. doi: 10.1038/s41556-023-01130-y."
COMMENT     Sequence Label: pLenti-CMV-PM
FEATURES             Location/Qualifiers
     source          1..9762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1512..1565
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2160..2189
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(2661..2685)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2687..2728
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2770..3358
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2823..2843)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3241..3252)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3430..3663
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3741..3862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3778..3797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3832..3851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3902..4037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4057..4076)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4058..4076)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4086..4103)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4086..4102)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4095..4117)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4244..4699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4331..4350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4541..4562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4725..4829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4830..4898,4899..5690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5048..5067)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5861..6449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6350..6369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6603..6620
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6737..6758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6773..6778,6779..6796,6797..6803)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6811..6827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6816..6838
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6835..6851
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6835..6851
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6870..6890
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6872..6890
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6918..7144
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7145..7325
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7372..7497
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7990..8223
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8408..8452
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8627..9127
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9046..9064
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9139..9534
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9594..9711
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt cgacgccgcc accatgaaca
      661 gtgaggagca gtactacgcg gccacacagc tctacaagga cccgtgcgca ttccagaggg
      721 gcccggtgcc agagttcagc gctaaccccc ctgcgtgcct gtacatgggc cgccagcccc
      781 cacctccgcc gccaccccag tttacaagct cgctgggatc actggagcag ggaagtcctc
      841 cggacatctc cccatacgaa gtgcccccgc tcgcctccga cgacccggct ggcgctcacc
      901 tccaccacca ccttccagct cagctcgggc tcgcccatcc acctcccgga cctttcccgt
      961 atggaaccga gcctgggggc ctggaagagc ccaaccgcgt ccagctccct ttcccgtgga
     1021 tgaaatccac caaagctcac gcgtggaaag gccagtgggc aggaggtgct tacacagcgg
     1081 aacccgagga aaacaagagg acccgtactg cctacacccg ggcgcagctg ctggagctgg
     1141 agaaggaatt cttatttaac aaatacatct cccggccccg ccgggtggag ctggcagtga
     1201 tgttgaactt gaccgagaga cacatcaaaa tctggttcca aaaccgtcgc atgaagtgga
     1261 aaaaagagga agataagaaa cgtagtagcg ggaccccgag tgggggcggt gggggcgaag
     1321 agccggaaca agattgtgcg gtgacctcgg gcgaggagct gctggcagtg ccaccgctgc
     1381 cacctcccgg aggtgccgtg cccccaggcg tcccagctgc agtccgggag ggcctactgc
     1441 cttcgggcct tagcgtgtcg ccacagccct ccagcatcgc gccactgcga ccgcaggaac
     1501 cccggggatc cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     1561 gccctatcga tatggccgcg gagctggcga tgggcgcaga gctgcccagc agcccactgg
     1621 ccatcgagta cgtcaacgac ttcgacctga tgaagttcga ggtgaagaag gagccgcccg
     1681 aggccgagcg cttctgccac cgcctgccgc ccggctcgct gtcctcgacg cccctcagca
     1741 cgccctgctc ctcggtgccc tcttcgccca gcttctgcgc acccagcccg ggcacaggcg
     1801 gcggcgcggg cggcgggggc agcgcggctc aggccggggg cgccccgggg ccgccgagtg
     1861 gaggccccgg cactgtcggg ggcgcctcag gaaaagcggt gctggaggat ctgtactgga
     1921 tgagcgggta ccagcaccac ctgaaccccg aggcgctcaa cctgacgccg gaggacgcgg
     1981 tggaggcgct catcggcagc ggccaccacg gcgcgcacca cggcgcgcat cacccggcgg
     2041 ctgctgcggc ctatgaggcc ttccggggtc agagcttcgc gggcggcggc ggcgcggacg
     2101 acatgggtgc cggccaccac cacggcgcac accacactgc ccaccatcat cactctgccc
     2161 accatcacca tcaccaccat caccaccacg gaggctctgg ccaccacggc ggaggcgcgg
     2221 gtcacggcgg aggcggcgca ggccaccacg tgcgcttgga ggagcgcttc tccgacgacc
     2281 agctggtatc catgtccgtg cgggagctga accggcagct ccgcggcttc agcaaggagg
     2341 aggtcatccg actgaaacag aagcggcgca cgctcaagaa ccgcggctac gcgcagtcgt
     2401 gccgcttcaa gcgggtgcag cagcggcaca ttctggagag cgagaagtgc cagctccaga
     2461 gccaggtgga gcagctgaag ctggaggtgg ggcgtctggc caaggagcgg gacctgtaca
     2521 aggagaaata cgagaagttg gcgggccggg gcggccccgg gggcgcgggc ggggccggct
     2581 tccctcggga gccctcgcca gcgcaggctg gccccggggc ggccaaaggc gcacccgact
     2641 tctttctgta agatatctag acccagcttt cttgtacaaa gtggttggta agcctatccc
     2701 taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc ggtggcgcgt
     2761 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2821 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2881 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2941 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3001 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3061 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3121 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3181 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3241 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3301 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     3361 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3421 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     3481 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3541 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3601 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3661 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     3721 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     3781 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3841 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     3901 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3961 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4021 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     4081 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4141 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4201 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     4261 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4321 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4381 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4441 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4501 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4561 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4621 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4681 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4741 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4801 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4861 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4921 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4981 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5041 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5101 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5161 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5221 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5281 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5341 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5401 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5461 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5521 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5581 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5641 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5701 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5761 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5821 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5881 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5941 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6001 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6061 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6121 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6181 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6241 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6301 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6361 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6421 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6481 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6541 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6601 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6661 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6721 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6781 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6841 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     6901 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     6961 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     7021 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     7081 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     7141 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7201 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7261 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7321 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     7381 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     7441 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     7501 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     7561 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     7621 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     7681 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     7741 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     7801 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     7861 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7921 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     7981 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     8041 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     8101 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     8161 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     8221 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     8281 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     8341 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8401 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8461 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     8521 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     8581 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccttgc gccttttcca
     8641 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     8701 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     8761 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     8821 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
     8881 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
     8941 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
     9001 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
     9061 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     9121 tccccagggg gtaccaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     9181 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     9241 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     9301 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     9361 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     9421 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     9481 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggcctgcag
     9541 ctgcagtagt aagaattcta gatcttgaga caaatggcag tattcatcca caattttaaa
     9601 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     9661 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     9721 tacagggaca gcagagatcc actttggcgc cggctcgagg gg
//
