LOCUS       sequence-385592-        1988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries a fragment from a 24-fragment lacI/lacZ 
            cassette split that was designed as a Golden Gate assembly with high
            fidelity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, 
            Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
  TITLE     Comprehensive Profiling of Four Base Overhang Ligation Fidelity by 
            T4 DNA Ligase and Application to DNA Assembly.
  JOURNAL   ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 
            10.1021/acssynbio.8b00333. Epub 2018 Oct 29.
  PUBMED    30335370
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00333"; journalName: "ACS Synth Biol"; date: 
            "2018-11-16- 16"; volume: "7"; issue: "11"; pages: "2665-2674"
COMMENT     Sequence Label: pUC57-mini v2-HF24_F19
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(233..821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(313..332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(992..1783,1784..1852))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1615..1634
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1853..1957)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatgg tctcggcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
       61 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
      121 atttcttgat gtctctgacc agacacccat caaccgagac catcggaaag aacatgtgag
      181 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      241 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      301 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      361 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      421 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      481 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      541 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      601 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      661 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      721 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      781 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      841 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      901 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      961 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1021 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1081 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagatccac
     1141 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1201 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1261 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1321 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1381 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1441 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1501 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1561 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1621 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1681 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1741 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1801 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     1861 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1921 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     1981 ctgacgtc
//
