LOCUS       sequence-385664-       10168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and 
            TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Tou CJ, Orr B, Kleinstiver BP
  TITLE     Precise cut-and-paste DNA insertion using engineered type V-K 
            CRISPR-associated transposases.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01574-x.
  PUBMED    36593413
REFERENCE   2  (bases 1 to 10168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01574-x."
COMMENT     Sequence Label: pN7HELIX
FEATURES             Location/Qualifiers
     source          1..10168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2368..2388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2398..2451
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(3315..3889,3890..3892,3893..3901)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3482..3499)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3709..3728
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3905..3931
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3947..3967
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4451..4460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4490..4543
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4544..4570
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4586..4606
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7183..7200
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7223..7240)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7229..7453
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7524..7540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7524..7540)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7537..7559)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7548..7564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7572..7578,7579..7596,7597..7602))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7617..7638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7755..7772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7926..8514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8006..8025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8685..9476,9477..9545))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9308..9327
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9546..9650)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9729..9748)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9920..10168,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagcgtgatc
      421 accatccagt gcagactggt ggccgaagag gacatcctga gacagctgtg ggagctgatg
      481 gccgacaaga acacccctct gatcaacgag ctgctggccc aagtgggaaa gcaccccgag
      541 tttgagacat ggctggacaa gggcagaatc cccaccaagc tgctgaaaac cctggtcaac
      601 agcttcaaga cccaagagag attcgccgac cagcctggca gattctacac ctctgccatt
      661 gctctggtgg actacgtgta caagagttgg ttcgccctgc agaagcggcg gaagagacag
      721 atcgagggca aagagagatg gctgaccatc ctgaagtccg acctgcagct ggaacaagag
      781 tcccagtgta gcctgagcgc catcaggacc aaggccaacg agatcctgac acagttcacc
      841 cctcagagcg agcagaacaa gaaccagcgg aagggcaaaa agaccaagaa gtccaccaag
      901 tccgagaagt ccagcctgtt ccagatcctg ctgaacacct acgagcagac ccagaatcct
      961 ctgaccagat gcgccattgc ctacctgctg aagaacaact gccagatcag cgagctggac
     1021 gaggacagcg aggaattcac caagaaccgc cggaagaaag agattgagat cgagcgcctg
     1081 aagaatcagc tgcagagcag gatccctaag ggcagagatc tgaccggcga ggaatggctc
     1141 aagaccctgg aaatcagcac cgccaacgtg ccccagaacg agaatgaagc caaggcctgg
     1201 caagccgctc tgctgagaaa aagcgccgac gtgccatttc ctgtggccta cgagagcaac
     1261 gaggacatga cctggctgca gaacgacaaa ggcagactgt tcgtgcggtt caacggcctg
     1321 ggcaagctga ccttcgagat ctactgcgac aagcggcatc tgcactactt caagcggttt
     1381 ctcgaggacc aagagctgaa gcggaaccac aagaatcagt acagcagctc cctgttcacc
     1441 ctgcggagtg gtagacttgc ttggagccct ggcgaggaaa aaggcgagcc ctggaaagtg
     1501 aaccagctgc acctgtactg caccctggac accagaatgt ggaccatcga gggaacccag
     1561 caggtcgtgg acgagaaaag caccaagatc aacgaaaccc tgacaaaggc caagcagaag
     1621 gacgacctga acgaccagca gcaggccttc atcaccagac agcagagcac actggaccgg
     1681 atcaacaatc tgttccccag acctagcaag agcagatacc agggccagcc ttctatcctc
     1741 gtgggcgtgt ccttcggcct gaaaaagcct gtgacagtgg ccgtggtgga cgtggtcaag
     1801 aatgaggtgc tggcctacag aagcgtgaaa cagctgctgg gcgagaacta caatctgctg
     1861 aaccggcagc gacagcagca gcagagactg tctcacgaga gacacaaggc ccagaagcag
     1921 aacgccccta acagctttgg cgagtctgag ctgggccagt acatcgacag actgctggct
     1981 gacgccatca ttgccattgc caagacatac caggccggct ccatcgtgct gcccaagctg
     2041 agagatatga gagagcagat cagcagcgag atccagagca gagccgagaa gaagtgcccc
     2101 ggctacaaag aggtgcagca gaagtacgcc aaagaatacc ggatgagcgt gcaccggtgg
     2161 tcctacggca gactgatcga gtgcatcaag agccaggccg ccaaggccgg aatctctaca
     2221 gagatcggca cccagcctat ccggggctct cctcaagaga aggccagaga tgtggccgtg
     2281 ttcgcctacc aagaaagaca ggccgctctg atcagcggca gcgagacccc cggcaccagc
     2341 gagagcgcca cccccgagag cggcagccca aagaagaagc ggaaggtcgg aagcggcgaa
     2401 ggcagaggca gcctgcttac atgtggcgac gtggaagaga accccggacc taaggacgac
     2461 tactggcaga gatgggtgca gaacctgtgg ggcgacgagc ccattcctga agaactgcag
     2521 cccgagatcg agagactgct gagcccttct gtggtggaac tggaacacat ccagaagatc
     2581 cacgactggc tggacggcct gagactgtct aagcagtgcg gcagaattgt ggcccctcct
     2641 agagccggca agagcgtgac atgtgacgtg taccggctgc tgaacaagcc ccagaagaga
     2701 ggcggcaagc gggatattgt gcccgtgctg tatatgcagg tccccggcga ttgctctagc
     2761 ggagaactgc tggtgctgat cctggaaagc ctgaagtacg atgccaccag cggcaagctg
     2821 accgacctga gaagaagagt gcagcgcctg ctgaaagaaa gcaaggtgga aatgctgatt
     2881 atcgacgagg ccaacttcct caagctgaac accttcagcg agatcgcccg gatctacgac
     2941 ctgctgagaa tcagcatcgt gctcgtgggc accgacggcc tggacaacct gattaagaga
     3001 gagccctaca tccacgaccg gttcatcgag tgctacaagc tgcccctggt ggaaagcgag
     3061 aagaaattca ccgagctggt caagatctgg gaagaagagg tgctctgcct gcctctgcct
     3121 agcaacctga ccagaagcga gacactggaa cccctgcgga gaaagaccgg cggaaagatc
     3181 ggactggtgg acagagtgct gcggagagcc tctattctgg ccctgagaaa gggcctgaag
     3241 aatatcgaca aagaaaccct gaccgaggtg ctggattggt tcgagtgacg agctgtacaa
     3301 gtaaagcggc cgcgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     3361 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     3421 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     3481 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     3541 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     3601 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     3661 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     3721 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     3781 ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     3841 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     3901 catgtatccg tatgatgttc cggattatgc aggtggcgga agcggcccaa agaagaagcg
     3961 gaaggtcggt ggcggaagcg gcgaaatcgg agccgaggaa ccccacatct tcgaggtgga
     4021 acctctggaa ggcgagagcc tgtctcactt cctgggcaga ttcagaagag agaactacct
     4081 gaccagcagc cagctgggca agctgacagg actgggagct gtggtgtccc ggtggaagaa
     4141 gctgtacttc aacccatttc caacgcggca agagctggaa gccctgacct ctgtcgtcag
     4201 agtgaacgcc gatagactgg ccgagatgct gcctcctaag ggcgtgacca tgaagcccag
     4261 acctatcaga ctgtgcgccg cctgttatgc cgaggtgccc tgtcacagaa tcgagtggca
     4321 gttcaaggac gtgatgaagt gcgaccggca caacctgaga ctgctgacca agtgcaccaa
     4381 ctgcgagaca agcttcccca ttcctgccga atgggtgcag ggcgagtgcc ctcactgctt
     4441 tctgcctttt gccaccatgg ccaagcggca gaaacacggc ggaagcggcg aaggcagagg
     4501 cagcctgctt acatgtggcg acgtggaaga gaaccccgga ccttatccgt atgatgttcc
     4561 ggattatgca ggtggcggaa gcggcccaaa gaagaagcgg aaggtcggtg gcggaagcgg
     4621 cggcagcgac ctgacctacg cctacctggt gggcctgttc gagggcgacg gctacttcag
     4681 catcaccaag aagggcaagt acctgaccta cgagctgggc atcgagctga gcatcaagga
     4741 cgtgcagctg atctacaaga tcaagaagat cctgggcatc ggcatcgtga gcttcagaaa
     4801 gagaaacgag atcgagatgg tggccctgag aatcagagac aagaaccacc tgaagagcaa
     4861 gatcctgccc atcttcgaga agtaccccat gttcagcaac aagcagtacg actacctgag
     4921 attcagaaac gccctgctga gcggcatcat cagcctggag gacctgcccg actacaccag
     4981 aagcgacgag cccctgaaca gcatcgagag catcatcaac accagctact tcagcgcctg
     5041 gctggtgggc ttcatcgagg ccgagggctg cttcagcgtg tacaagctga acaaggacga
     5101 cgactacctg atcgccagct tcgacatcgc ccagagagac ggcgacatcc tgatcagcgc
     5161 catcagaaag tacctgagct tcaccaccaa ggtgtacctg gacaagacca actgcagcaa
     5221 gctgaaggtg accagcgtga gaagcgtgga gaacatcatc aagttcctgc agaacgcccc
     5281 cgtgaagctg ctgggcaaca tgaagctgca gtacaagctg tggctgaagc agctgagaaa
     5341 gatcagcaga tacagcgaga agatcaagat ccccagcaac tacagcggca gcgagacccc
     5401 cggcaccagc gagagcgcca cccccgagag cggcagcgac gagatgccca tcgtgaagca
     5461 ggacgacgag agcctgcctg tggaaaacaa cgacgatgtg gatgagatcc aggacgatga
     5521 gctggaagag acaaacgtga tcttcaccga gctgagcgcc gaggccaagc tgaagatgga
     5581 tgtgattcag ggcctgctgg aaccctgcga cagaaagaca tacggcgaga agctgagagt
     5641 ggccgccgag aaactgggaa agacagtgcg gacagtgcag cggctggtca agaagtatca
     5701 gcaggacggc ctgagcgcca tcgtggaaac ccagagaaac gacaagggca gctaccggat
     5761 cgaccccgag tggcagaaat tcatcgtgaa caccttcaaa gagggcaaca agggctccaa
     5821 gaagatgacc cctgctcagg tggccatgag agtgcaagtt cgggctgaac agctgggcct
     5881 gcagaaattt cccagccaca tgaccgtgta ccgggtgctg aaccccatca tcgagcggca
     5941 agagcggaag cagaagcaga gaaacatcgg ctggcggggc agcagagtgt cccacaagac
     6001 aagagatggc cagacactgg acgtgcggta cagcaatcac gtgtggcagt gcgaccacac
     6061 caagctggat gtcatgctgg tggaccagta cggcgagcct cttgccagac catggttcac
     6121 caagatcacc gacagctaca gccggtgcat catgggcatc cacgtgggct ttgatgcccc
     6181 tagctctcag gttgtggccc tggcctctag acacgccatt ctgcctaagc agtacagcgc
     6241 cgagtacaaa ctgatcagcg actggggcac ctacggcgtg cccgagaatc tgtttacaga
     6301 cggcggcaga gacttcagaa gcgagcacct gaagcagatc ggcttccagc tgggcttcga
     6361 gtgtcacctg agagacagac ctagcgaagg cggcatcgag gaaagaagct tcggaacaat
     6421 caataccgag ttcctgagcg gcttctacgg ctacctgggc agcaacatcc aagagagaag
     6481 caagaccgcc gaggaagagg cctgtctgac actgagagag ctgcatctgc tgctcgtgcg
     6541 ctacatcgtg gacaactaca accagaggct ggacgcccgg accaaggacc agaccagatt
     6601 tcagagatgg gaggccggac tgcctgctct gcccaagatg gtcaaagagc gcgagctgga
     6661 catctgcctg atgaagaaaa cccggcggag catctacaaa ggcggctatc tgagcttcga
     6721 gaacatcatg taccggggcg attacctggc cgcctatgcc ggcgagaata tcgtgctgag
     6781 atacgacccc agagacatca ccaccgtgtg ggtgtacaga atcgataagg gcaaagaggt
     6841 gttcctgtcc gccgctcatg ccctggattg ggagacagaa cagctgtccc tggaagaagc
     6901 caaggccgcc tctagaaaag tgcggagcgt gggcaagacc ctgagcaaca agtctatcct
     6961 ggccgagatc cacgaccggg acacctttat caagcagaag aagaagtccc agaaagagcg
     7021 caagaaagag gaacaggccc aggtccacgc cgtgtacgag cctatcaatc tgagcgagac
     7081 agagcccctg gaaaacctgc aagagacacc caagcctgtg accagaaagc cccggatctt
     7141 caactacgag cagctgcggc aggactacga cgagtgaccg gtcatcatca ccatcaccat
     7201 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7261 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7321 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7381 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7441 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc gtcgacctct
     7501 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7561 acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg tgcctaatga
     7621 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7681 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7741 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7801 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7861 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7921 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7981 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8041 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8101 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8161 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8221 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8281 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8341 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8401 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8461 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8521 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8581 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8641 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8701 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8761 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8821 ccgcgcgaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8881 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8941 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9001 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9061 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9121 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9181 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9241 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9301 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9361 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9421 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9481 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9541 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9601 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9661 cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc cctagggtcg
     9721 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     9781 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     9841 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     9901 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     9961 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
    10021 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
    10081 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
    10141 gcccacttgg cagtacatca agtgtatc
//
