LOCUS       sequence-385667-        9352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and 
            TniQ-T2A-TnsB are separated by an EMCV IRES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Tou CJ, Orr B, Kleinstiver BP
  TITLE     Precise cut-and-paste DNA insertion using engineered type V-K 
            CRISPR-associated transposases.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01574-x.
  PUBMED    36593413
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01574-x."
COMMENT     Sequence Label: pN7CAST
FEATURES             Location/Qualifiers
     source          1..9352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2368..2388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2398..2451
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(3315..3889,3890..3892,3893..3901)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3482..3499)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3709..3728
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3905..3931
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3947..3967
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4451..4460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4490..4543
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4544..4570
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4586..4606
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6367..6384
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6407..6424)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6413..6637
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6708..6724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6708..6724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6721..6743)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6732..6748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6756..6762,6763..6780,6781..6786))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6801..6822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6939..6956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7110..7698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7190..7209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7869..8660,8661..8729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8492..8511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8730..8834)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8913..8932)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9104..9352,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagcgtgatc
      421 accatccagt gcagactggt ggccgaagag gacatcctga gacagctgtg ggagctgatg
      481 gccgacaaga acacccctct gatcaacgag ctgctggccc aagtgggaaa gcaccccgag
      541 tttgagacat ggctggacaa gggcagaatc cccaccaagc tgctgaaaac cctggtcaac
      601 agcttcaaga cccaagagag attcgccgac cagcctggca gattctacac ctctgccatt
      661 gctctggtgg actacgtgta caagagttgg ttcgccctgc agaagcggcg gaagagacag
      721 atcgagggca aagagagatg gctgaccatc ctgaagtccg acctgcagct ggaacaagag
      781 tcccagtgta gcctgagcgc catcaggacc aaggccaacg agatcctgac acagttcacc
      841 cctcagagcg agcagaacaa gaaccagcgg aagggcaaaa agaccaagaa gtccaccaag
      901 tccgagaagt ccagcctgtt ccagatcctg ctgaacacct acgagcagac ccagaatcct
      961 ctgaccagat gcgccattgc ctacctgctg aagaacaact gccagatcag cgagctggac
     1021 gaggacagcg aggaattcac caagaaccgc cggaagaaag agattgagat cgagcgcctg
     1081 aagaatcagc tgcagagcag gatccctaag ggcagagatc tgaccggcga ggaatggctc
     1141 aagaccctgg aaatcagcac cgccaacgtg ccccagaacg agaatgaagc caaggcctgg
     1201 caagccgctc tgctgagaaa aagcgccgac gtgccatttc ctgtggccta cgagagcaac
     1261 gaggacatga cctggctgca gaacgacaaa ggcagactgt tcgtgcggtt caacggcctg
     1321 ggcaagctga ccttcgagat ctactgcgac aagcggcatc tgcactactt caagcggttt
     1381 ctcgaggacc aagagctgaa gcggaaccac aagaatcagt acagcagctc cctgttcacc
     1441 ctgcggagtg gtagacttgc ttggagccct ggcgaggaaa aaggcgagcc ctggaaagtg
     1501 aaccagctgc acctgtactg caccctggac accagaatgt ggaccatcga gggaacccag
     1561 caggtcgtgg acgagaaaag caccaagatc aacgaaaccc tgacaaaggc caagcagaag
     1621 gacgacctga acgaccagca gcaggccttc atcaccagac agcagagcac actggaccgg
     1681 atcaacaatc tgttccccag acctagcaag agcagatacc agggccagcc ttctatcctc
     1741 gtgggcgtgt ccttcggcct gaaaaagcct gtgacagtgg ccgtggtgga cgtggtcaag
     1801 aatgaggtgc tggcctacag aagcgtgaaa cagctgctgg gcgagaacta caatctgctg
     1861 aaccggcagc gacagcagca gcagagactg tctcacgaga gacacaaggc ccagaagcag
     1921 aacgccccta acagctttgg cgagtctgag ctgggccagt acatcgacag actgctggct
     1981 gacgccatca ttgccattgc caagacatac caggccggct ccatcgtgct gcccaagctg
     2041 agagatatga gagagcagat cagcagcgag atccagagca gagccgagaa gaagtgcccc
     2101 ggctacaaag aggtgcagca gaagtacgcc aaagaatacc ggatgagcgt gcaccggtgg
     2161 tcctacggca gactgatcga gtgcatcaag agccaggccg ccaaggccgg aatctctaca
     2221 gagatcggca cccagcctat ccggggctct cctcaagaga aggccagaga tgtggccgtg
     2281 ttcgcctacc aagaaagaca ggccgctctg atcagcggca gcgagacccc cggcaccagc
     2341 gagagcgcca cccccgagag cggcagccca aagaagaagc ggaaggtcgg aagcggcgaa
     2401 ggcagaggca gcctgcttac atgtggcgac gtggaagaga accccggacc taaggacgac
     2461 tactggcaga gatgggtgca gaacctgtgg ggcgacgagc ccattcctga agaactgcag
     2521 cccgagatcg agagactgct gagcccttct gtggtggaac tggaacacat ccagaagatc
     2581 cacgactggc tggacggcct gagactgtct aagcagtgcg gcagaattgt ggcccctcct
     2641 agagccggca agagcgtgac atgtgacgtg taccggctgc tgaacaagcc ccagaagaga
     2701 ggcggcaagc gggatattgt gcccgtgctg tatatgcagg tccccggcga ttgctctagc
     2761 ggagaactgc tggtgctgat cctggaaagc ctgaagtacg atgccaccag cggcaagctg
     2821 accgacctga gaagaagagt gcagcgcctg ctgaaagaaa gcaaggtgga aatgctgatt
     2881 atcgacgagg ccaacttcct caagctgaac accttcagcg agatcgcccg gatctacgac
     2941 ctgctgagaa tcagcatcgt gctcgtgggc accgacggcc tggacaacct gattaagaga
     3001 gagccctaca tccacgaccg gttcatcgag tgctacaagc tgcccctggt ggaaagcgag
     3061 aagaaattca ccgagctggt caagatctgg gaagaagagg tgctctgcct gcctctgcct
     3121 agcaacctga ccagaagcga gacactggaa cccctgcgga gaaagaccgg cggaaagatc
     3181 ggactggtgg acagagtgct gcggagagcc tctattctgg ccctgagaaa gggcctgaag
     3241 aatatcgaca aagaaaccct gaccgaggtg ctggattggt tcgagtgacg agctgtacaa
     3301 gtaaagcggc cgcgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     3361 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     3421 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     3481 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     3541 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     3601 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     3661 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     3721 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     3781 ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     3841 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     3901 catgtatccg tatgatgttc cggattatgc aggtggcgga agcggcccaa agaagaagcg
     3961 gaaggtcggt ggcggaagcg gcgaaatcgg agccgaggaa ccccacatct tcgaggtgga
     4021 acctctggaa ggcgagagcc tgtctcactt cctgggcaga ttcagaagag agaactacct
     4081 gaccagcagc cagctgggca agctgacagg actgggagct gtggtgtccc ggtggaagaa
     4141 gctgtacttc aacccatttc caacgcggca agagctggaa gccctgacct ctgtcgtcag
     4201 agtgaacgcc gatagactgg ccgagatgct gcctcctaag ggcgtgacca tgaagcccag
     4261 acctatcaga ctgtgcgccg cctgttatgc cgaggtgccc tgtcacagaa tcgagtggca
     4321 gttcaaggac gtgatgaagt gcgaccggca caacctgaga ctgctgacca agtgcaccaa
     4381 ctgcgagaca agcttcccca ttcctgccga atgggtgcag ggcgagtgcc ctcactgctt
     4441 tctgcctttt gccaccatgg ccaagcggca gaaacacggc ggaagcggcg aaggcagagg
     4501 cagcctgctt acatgtggcg acgtggaaga gaaccccgga ccttatccgt atgatgttcc
     4561 ggattatgca ggtggcggaa gcggcccaaa gaagaagcgg aaggtcggtg gcggaagcgg
     4621 cgacgagatg cccatcgtga agcaggacga cgagagcctg cctgtggaaa acaacgacga
     4681 tgtggatgag atccaggacg atgagctgga agagacaaac gtgatcttca ccgagctgag
     4741 cgccgaggcc aagctgaaga tggatgtgat tcagggcctg ctggaaccct gcgacagaaa
     4801 gacatacggc gagaagctga gagtggccgc cgagaaactg ggaaagacag tgcggacagt
     4861 gcagcggctg gtcaagaagt atcagcagga cggcctgagc gccatcgtgg aaacccagag
     4921 aaacgacaag ggcagctacc ggatcgaccc cgagtggcag aaattcatcg tgaacacctt
     4981 caaagagggc aacaagggct ccaagaagat gacccctgct caggtggcca tgagagtgca
     5041 agttcgggct gaacagctgg gcctgcagaa atttcccagc cacatgaccg tgtaccgggt
     5101 gctgaacccc atcatcgagc ggcaagagcg gaagcagaag cagagaaaca tcggctggcg
     5161 gggcagcaga gtgtcccaca agacaagaga tggccagaca ctggacgtgc ggtacagcaa
     5221 tcacgtgtgg cagtgcgacc acaccaagct ggatgtcatg ctggtggacc agtacggcga
     5281 gcctcttgcc agaccatggt tcaccaagat caccgacagc tacagccggt gcatcatggg
     5341 catccacgtg ggctttgatg cccctagctc tcaggttgtg gccctggcct ctagacacgc
     5401 cattctgcct aagcagtaca gcgccgagta caaactgatc agcgactggg gcacctacgg
     5461 cgtgcccgag aatctgttta cagacggcgg cagagacttc agaagcgagc acctgaagca
     5521 gatcggcttc cagctgggct tcgagtgtca cctgagagac agacctagcg aaggcggcat
     5581 cgaggaaaga agcttcggaa caatcaatac cgagttcctg agcggcttct acggctacct
     5641 gggcagcaac atccaagaga gaagcaagac cgccgaggaa gaggcctgtc tgacactgag
     5701 agagctgcat ctgctgctcg tgcgctacat cgtggacaac tacaaccaga ggctggacgc
     5761 ccggaccaag gaccagacca gatttcagag atgggaggcc ggactgcctg ctctgcccaa
     5821 gatggtcaaa gagcgcgagc tggacatctg cctgatgaag aaaacccggc ggagcatcta
     5881 caaaggcggc tatctgagct tcgagaacat catgtaccgg ggcgattacc tggccgccta
     5941 tgccggcgag aatatcgtgc tgagatacga ccccagagac atcaccaccg tgtgggtgta
     6001 cagaatcgat aagggcaaag aggtgttcct gtccgccgct catgccctgg attgggagac
     6061 agaacagctg tccctggaag aagccaaggc cgcctctaga aaagtgcgga gcgtgggcaa
     6121 gaccctgagc aacaagtcta tcctggccga gatccacgac cgggacacct ttatcaagca
     6181 gaagaagaag tcccagaaag agcgcaagaa agaggaacag gcccaggtcc acgccgtgta
     6241 cgagcctatc aatctgagcg agacagagcc cctggaaaac ctgcaagaga cacccaagcc
     6301 tgtgaccaga aagccccgga tcttcaacta cgagcagctg cggcaggact acgacgagtg
     6361 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
     6421 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6481 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6541 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6601 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6661 tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6721 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6781 tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6841 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6901 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7801 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7861 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7921 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7981 tctggcccca gtgctgcaat gataccgcgc gaaccacgct caccggctcc agatttatca
     8041 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8101 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8161 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8221 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8281 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8341 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8401 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8461 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8521 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8581 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8641 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8701 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8761 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8821 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8881 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
     8941 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     9001 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     9061 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     9121 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     9181 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     9241 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     9301 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tc
//
