LOCUS       sequence-385679-        9363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses eGFP mRNA from the Drosophila Ana promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Fujiwara R, Zhai SN, Liang D, Shah AP, Tracey M, Ma XK, Fields CJ, 
            Mendoza-Figueroa MS, Meline MC, Tatomer DC, Yang L, Wilusz JE
  TITLE     IntS6 and the Integrator phosphatase module tune the efficiency of 
            select premature transcription termination events.
  JOURNAL   Mol Cell. 2023 Nov 14:S1097-2765(23)00902-4. doi: 
            10.1016/j.molcel.2023.10.035.
  PUBMED    37995689
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2023 Nov 14:S1097-2765(23)00902-4. doi: 
            10.1016/j.molcel.2023.10.035."
COMMENT     Sequence Label: Hy_pAna eGFP SV40
FEATURES             Location/Qualifiers
     source          1..9363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(803..1082)
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     primer_bind     complement(2478..2496)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2564..2668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2669..2737,2738..3529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2887..2906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3700..4288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4189..4208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4442..4459
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5169..5290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(5299..6012,6013..6015,6016..6018))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5302..6012,6013..6015,6016..6018))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5344..5365)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5693..5712
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5952..5973
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6036..6052)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6037..6053)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    7971..8092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8517..8537)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8667..8732
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(join(9095..9363,1..757))
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(join(9095..9363,1..757))
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
       61 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
      121 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
      181 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
      241 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
      301 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
      361 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
      421 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
      481 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
      541 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
      601 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
      661 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
      721 tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc cggacgagcg
      781 ccaagcttgg ctgcaggtcc ataacctgtt gtaatttata atttatattt cctttcttaa
      841 taaataaata aatagtcaag tttatgtttg agttttatga tttatatttt aagttatttc
      901 aactgcaaca ccagcaccac gacctactta cagcaaaaaa cgtacaagaa ggaaagaagg
      961 aataaaaaga gtggtattct cttacaatat gttttatggc ataaaaggtg tggccattca
     1021 tatcaaatat aaagtagtgt tgtttaacgt tacttttgta ggttgaatag tatattccaa
     1081 cagatgatga ggggttccca atcctaaacc catttgccgt tcccagaagc atgaaaccac
     1141 cacgcaccgg aaacaacgtt aaatgcgtaa caaggcgctt tttaatttaa ccgatccaaa
     1201 taaaagaatt aaataaatgg aattgtgtcc acatgcaaag cagttggagg acagcagcgt
     1261 tatcagcatt tcgaagcgca cgaaaacgca tgacgaacgg gcggcttaat tgaaattgtc
     1321 attgattcaa tacgcaacat gcagaggagc atatcatgat ggtcggttcg ttcttcgttt
     1381 cactgggaaa aagcgaaatt gaatccattt tgattgcgtt tgtgtcgcaa gagcaacaca
     1441 ttgcctgcgc cacatcgcgt tcgcttatca acgggcgaag aaaggaaaat gtacatttaa
     1501 ttggtcgtaa aaaagtgcat tgtaaatgtt aaagtcgctg atagcttcgg gccgcttgtt
     1561 cgcaatgata acgacagtgt aacaaattcc agaggggagc cccttccaaa atacgagatt
     1621 ccaaaaaaaa aaaaacaaaa aaaagaagaa gcgtttcgaa actctgaaga aaacccagag
     1681 aagataagag cgtacttgac agcatatagc aaaactattg tacatttcca aatttcaatt
     1741 ggaatcgaaa cgggaacagt caatcgcttg ggttatcgga ataatcgcct caattgcgtg
     1801 aacctatcga cacaaagtgc attggtattg agtcaaagta aggtttgctt atattaactt
     1861 tcatagattg catatttttg ccggcttatt catgggaata agaaatgttt gagctgaact
     1921 cgatagctca acaataacac gacttggtgt caattaaatg ataatacgtg actagaccat
     1981 ggaaattcaa tgtattgcta ttggatacaa tacatataca agtagttgtt gaaaagaaag
     2041 cagcgaaaac tggtcaggta atgagtcatc atcatcggag tcatcttgac tgattagcac
     2101 aaaagccagt tctcatctgt atgcaaatgg cttaaacgct tctacgtaca taagtagttg
     2161 atgttgttaa acaggaactt aagatgccgc caacaacagt ttccacagca attaaaccca
     2221 aatttttaat tgcgcataac aggcgtcttt catgttcctg tccgcatttg acattgatct
     2281 ccttctgaat cctgattcga taatatgtaa tcttggggcc atcaatcacg cagtcagcag
     2341 cgatttgata tatgtatata taacaccata ttattcaaag ccgtttgcct tgctcggttg
     2401 atgaggaaat tggtgaaaaa ctcgaacttt tgataaaaaa aaaattcgta caaatgtgaa
     2461 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2521 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2581 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2641 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2701 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2761 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2821 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2881 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     2941 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3001 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3061 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3121 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3181 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     3241 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3301 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3361 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3421 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3481 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3541 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3601 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3661 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3721 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3781 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3841 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3901 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3961 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4021 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4081 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4141 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4201 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4261 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4321 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4381 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4441 cagcgagtca gtgagcgagg aagcggaaga gcgctgactt ccgcgtttcc agactttacg
     4501 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
     4561 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
     4621 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc
     4681 caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     4741 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     4801 ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
     4861 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat
     4921 ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg
     4981 tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg
     5041 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     5101 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcggcggcc cctagtggat
     5161 ccactagtta agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     5221 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     5281 taaacaagtt gcggccgctt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
     5341 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     5401 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     5461 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     5521 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     5581 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     5641 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     5701 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     5761 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     5821 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     5881 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     5941 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     6001 ctcgcccttg ctcaccatgg tgatatcaag cttatcgata ccgtcgacct cgagttttgt
     6061 gttctgctct tggtctgttg ccaaactaaa tgttttcaca cggaaatagt tgtagttttc
     6121 ttcttcactt aaattcaacg tttgttcact gcatttttgc cagcaattaa aatgctatct
     6181 tttacacttc agctggatac ggacatgcga atttgttaat ttttgtagct gttgtgttca
     6241 gcttctcgat gtgttcgctc taagaaccaa gtatgaagtg atttgcccga gtcggttttt
     6301 tagtcttaaa taagaaaaat ttggttgtgc cgggcatatc tactttgtta tggtcgtaaa
     6361 cgctgcgttg cggccggcac tgtgagaaaa tgcgaccgcc ttaaaaagaa caacttactt
     6421 ttagctgata gcaaactatt aatatcttaa taaaattaca ttaaataata cacgtaatat
     6481 cttaacataa catttatcta tataataaag ggcttactta aataaattgg cacaaaataa
     6541 atattttact actggattaa tattatataa taatattgag attgaaaggt actattcaag
     6601 gtgacctgag cttgaaagcg tacattaaat cttagcttag ttctttgcac tagttttttt
     6661 tactttatat attattatcg catttgttcg ccactcaaaa ccccccaact aagtttgcag
     6721 tgtagcgctt ggggccttcg gctggcggtt cggctggcgc tcggatacac ggtggccgac
     6781 caatcaacgc cgcttgagga tctgccggcg ctgctgacgt cgacgtggtg ctctacagtg
     6841 gccgtgcgtg agatacgtac gaaacgcggc tgtatgggtg tatctgtatc tttggcctgt
     6901 gcctgcgcgc ccgagatggg gcttatcttt agggccgaaa gcgatactcc ggccctaacc
     6961 taggtttgtt tggcgacttc ctttcaagtg gctgctatca aacagggcca tcaaccgaaa
     7021 actgctaggc atatcaatct atctatctat tactccaaac actcggggca cttcaaacat
     7081 tatgaacaac ctgtgtcaat cttttggacg gctttgcacc catcggattc gttcgaatgt
     7141 ccgtaaagac gggcactatt tattcaatta ctacgtgcgt gggcgattag attaacctaa
     7201 gtgattttcc tcgcaagcgc acaattagta tgacctaatc aaattgtttt gatacgccag
     7261 ttatgtccgt tatcggttgc tgttattttt tggggcacga tggggcaaga tgcctttgct
     7321 cttgactttc agctgtcgcc ggcatttgtg cgtgcggact aatcatctga caacgagata
     7381 ggcccgaaag tgcgtagtcc atcaattgga aggtgaattg gacggatata tcctattaga
     7441 ggtgcgtgcg gtgcctggca cggctttggg catttggcgc ttaaccccag aaagcccatc
     7501 ctttggccca cttctggtac tacgcatgag gaatagcaca agcctcgaat attattcatt
     7561 ccattcgcat acggaaatcc aaacagccag acttcaagga cgttcctgat ttatttattt
     7621 atttgcaaca tataaacaca tcttttccag cactccgccg acgtctggac caaattgaat
     7681 ttcaaaatga ttgtataatt cgcagcactt tataaagtct ggattcggct tcttcccgct
     7741 gcccattcat gtgatgtgtc tacacacacc gtttaagttc tgaatagagt ggtgagtggg
     7801 gtcgcagggg cgggcttctc cagacgtcga gtgatatgtg ttatgtaaaa ttgcaatcgc
     7861 atcaaatgcc cccagattca taatcccgcc ggtaggtggg gtggtggtgt ggtgtggtgt
     7921 ggtgtggggg gaatatgaaa acaagatata tttaattaat ccagacatga taagatacat
     7981 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     8041 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     8101 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     8161 gtaaaacctc tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc
     8221 aaatattcct tattaacccc tttacaaatt aaaaagctaa aggtacacaa tttttgagca
     8281 tagttattaa tagcagacac tctatgcctg tgtggagtaa gaaaaaacag tatgttatga
     8341 ttataactgt tatgcctact tataaaggtt acagaatatt tttccataat tttcttgtat
     8401 agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc aagcaagagt tctattacta
     8461 aacacagcat gactcaaaaa acttagcaat tctgaaggaa agtccttggg gtcttctacc
     8521 tttctcttct tttttggagg agtagaatgt tgagagtcag cagtagcctc atcatcacta
     8581 gatggcattt cttctgagca aaacaggttt tcctcattaa aggcattcca ccactgctcc
     8641 cattcatcag ttccataggt tggaatctaa aatacacaaa caattagaat cagtagttta
     8701 acacattata cacttaaaaa ttttatattt accttagagc tttaaatctc tgtaggtagt
     8761 ttgtccaatt atgtcacacc acagaagtaa ggttccttca caaagatctg cacccaatcg
     8821 gcaggcacgg gcggcgatct ccaatctgcg ggatcagtca gatcacccga gtgcgtgggc
     8881 atgacaatcg tgccctgggg accaacacaa tccagaaggg cctgaatcac tgcgaccggc
     8941 cctcccgcga cccagccgag cgagcttagc gaactgtgga cgagaactgt gccaccaagc
     9001 gtaaggccgt tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt
     9061 ctcgaaatca gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
     9121 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     9181 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     9241 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     9301 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     9361 gat
//
