LOCUS       sequence-385695-        3822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BdRF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BdRF1
FEATURES             Location/Qualifiers
     source          1..3822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5..796)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     1227..1243
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1227..1243
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1248..1267
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1248..1266
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1284..1383
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2416..2515)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2531..2548)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2531..2547)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2540..2562)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2611..2697
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2789..2816
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2975..2992)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3146..3734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3226..3245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agggttattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
       61 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
      121 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
      181 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
      241 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
      301 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
      361 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
      421 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
      481 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
      541 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
      601 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
      661 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
      721 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
      781 tcaccgcttc cctcataatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
      841 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
      901 cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg
      961 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     1021 acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc
     1081 gtttccacgg tgtgcgtcac ccggcaacct tgggtagcag cgaagtcgag gcatttctgt
     1141 cctggctggt ctagaggagg catgatgata tatttttatc ttgtgcaatg taacatcaga
     1201 gattttgaga cacgggccag agctgccagg aaacagctat gaccatgtaa tacgactcac
     1261 tataggggat atcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt
     1321 cgttgcaaca aattgataag caatgctttc ttataatgcc aactttgtac aagaaagttg
     1381 gagccacgcg tttattcagc aattcctcgc aaaagatgga cttgaggtgg gctgacacag
     1441 acttggcctt gggggtggtg ggctccttag actgagccac tccggacgca gaggcctgca
     1501 ccagtttctt tccgcggtgg tggccctcgg tggtgggctg ctgagggttg gtgtcctccc
     1561 ggggtccaga gttcccggct gctcccgcct cagggatcgt ggactcggcc acacagtggg
     1621 cttcgggggc ctgtgtgccc gggggtggag gctgcgcctg agctgcctgg tgagaagttg
     1681 gtggtggggg cggatgaggg acgtatctgg gcacttcgta gctttgatga ggctgaaatc
     1741 cgtaaggccc cgagtgggtg tagcaggggc cctgctgggg gcagtatccg actccggtgt
     1801 ggccgtagga cagcgtctcc cggcgcaggg cctgtagcgt gtgctgtaac tcattcacac
     1861 tcttggagag gcccgctatg tccttgcggt agagggtggc gtcctccccc gggaatagcc
     1921 ccccgccttc ttcatcctcc tcggggtctc ttttccgctt gttggatcgc gagggagcgg
     1981 ggacgtagtc tgcccgcaag tgggatccgt agtaaggagg cacgggtgga ggggggccaa
     2041 agaggcccgg caagccaaag taggccggag gggggtgtgg gtgggggtgg taggtgtgag
     2101 cgtccatcgc caagaaggca cccgggtccc cgcccgcggg cccggcgtag taacctcctg
     2161 gggaggtaaa gtagccgccg gccggggcga ccgctccggc cccaggaccg taggaaggcg
     2221 cggtggctat tgcctggtgg gaaaaggggg gcagcggggc cgcgtagggt agcggggtct
     2281 gcctcggcgg tttgttgtcc aggttggtct gaagcagtgt cataaaggtg ttgcggggga
     2341 cgctgatgag gtcctggccc gcggcggccg aacctccgca ggctgttgct ccttctcctg
     2401 cgttacctga tagcatgcca acttttttgt acaaagttgg cattataaaa aagcattgct
     2461 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttggggcc
     2521 cgagcttaag actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg
     2581 ttaacgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac
     2641 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     2701 ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg
     2761 ccataaactg ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg
     2821 tttctacaaa ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg
     2881 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2941 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3001 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3061 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3781 acgcgtaact cacgttaagg gattttggtc atgggtggct cg
//
